Product: BMX Antibody
Catalog: AF4744
Description: Rabbit polyclonal antibody to BMX
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 76kDa; 78kD(Calculated).
Uniprot: P51813
RRID: AB_2844742

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
BMX Antibody detects endogenous levels of total BMX.
RRID:
AB_2844742
Cite Format: Affinity Biosciences Cat# AF4744, RRID:AB_2844742.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Bmx; BMX non receptor tyrosine kinase; BMX_HUMAN; BONE MARROW KINASE, X-LINKED; Bone marrow tyrosine kinase gene in chromosome X protein; Cytoplasmic tyrosine protein kinase BMX; Cytoplasmic tyrosine-protein kinase BMX; Epithelial and endothelial tyrosine kinase; ETK; NKT38; NTK 38; NTK38; Protein tyrosine kinase BMX; PSCTK 2; PSCTK 3; PSCTK2; PSCTK3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P51813 BMX_HUMAN:

Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines.

Sequence:
MDTKSILEELLLKRSQQKKKMSPNNYKERLFVLTKTNLSYYEYDKMKRGSRKGSIEIKKIRCVEKVNLEEQTPVERQYPFQIVYKDGLLYVYASNEESRSQWLKALQKEIRGNPHLLVKYHSGFFVDGKFLCCQQSCKAAPGCTLWEAYANLHTAVNEEKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKKNYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPDWWQVRKLKSSSSSEDVASSNQKERNVNHTTSKISWEFPESSSSEEEENLDDYDWFAGNISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVKHYHVHTNAENKLYLAENYCFDSIPKLIHYHQHNSAGMITRLRHPVSTKANKVPDSVSLGNGIWELKREEITLLKELGSGQFGVVQLGKWKGQYDVAVKMIKEGSMSEDEFFQEAQTMMKLSHPKLVKFYGVCSKEYPIYIVTEYISNGCLLNYLRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDRDLCVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVWAFGILMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIYQIMYSCWHELPEKRPTFQQLLSSIEPLREKDKH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51813 As Substrate

Site PTM Type Enzyme
Y40 Phosphorylation Q05397 (PTK2)
K172 Acetylation
K181 Acetylation
S186 Phosphorylation
Y216 Phosphorylation Q08881 (ITK) , Q06187 (BTK) , P51813 (BMX) , P42680 (TEC)
Y224 Phosphorylation Q08881 (ITK) , P42680 (TEC) , P51813 (BMX) , Q06187 (BTK)
S226 Phosphorylation
S254 Phosphorylation
S260 Phosphorylation
S261 Phosphorylation
S324 Phosphorylation
Y330 Phosphorylation
K337 Acetylation
K343 Acetylation
K347 Acetylation
Y365 Phosphorylation
T394 Phosphorylation
K395 Acetylation
K413 Ubiquitination
S425 Phosphorylation
Y440 Phosphorylation
Y566 Phosphorylation P12931 (SRC) , P51813 (BMX)
S568 Phosphorylation

PTMs - P51813 As Enzyme

Substrate Site Source
P08581 (MET) Y1235 Uniprot
P11362 (FGFR1) Y653 Uniprot
P11362 (FGFR1) Y654 Uniprot
P51813 (BMX) Y216 Uniprot
P51813 (BMX) Y224 Uniprot
P51813 (BMX) Y566 Uniprot
P56945 (BCAR1) Y12 Uniprot
Q05397 (PTK2) Y194 Uniprot
Q05397 (PTK2) Y441 Uniprot
Q05397 (PTK2) Y576 Uniprot
Q05397 (PTK2) Y577 Uniprot
Q05397 (PTK2) Y742 Uniprot
Q05397 (PTK2) Y861 Uniprot
Q05397 (PTK2) Y898 Uniprot
Q05397 (PTK2) Y925 Uniprot
Q05397 (PTK2) Y1007 Uniprot
Q07912 (TNK2) Y859 Uniprot
Q07912 (TNK2) Y860 Uniprot
Q16611 (BAK1) Y108 Uniprot
Q96T51 (RUFY1) Y389 Uniprot
Q96T51 (RUFY1) Y400 Uniprot

Research Backgrounds

Function:

Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.

PTMs:

Phosphorylated in response to protein I/II and to LPS. Phosphorylation at Tyr-566 by SRC and by autocatalysis leads to activation and is required for STAT3 phosphorylation by BMX.

Subcellular Location:

Cytoplasm.
Note: Localizes to the edges of spreading cells when complexed with BCAR1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines.

Subunit Structure:

Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B.

Family&Domains:

SH2 domain mediates interaction with RUFY1.

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

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