NUMB Antibody - #AF4735
| Product: | NUMB Antibody |
| Catalog: | AF4735 |
| Description: | Rabbit polyclonal antibody to NUMB |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 78kDa; 71kD(Calculated). |
| Uniprot: | P49757 |
| RRID: | AB_2844734 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF4735, RRID:AB_2844734.
Fold/Unfold
c14_5527; C14orf41; FLJ31314; h Numb; h-Numb; Numb; Numb homolog (Drosophila); Numb homolog; Numb protein homolog; NUMB_HUMAN; Protein numb homolog; Protein S171; S171;
Immunogens
- P49757 NUMB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKAERKFFKGFFGKTGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTGERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQMQDAKKAETDKIVVGSSVAPGNTAPSPSSPTSPTSDATTSLEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFKRQLSLRINELPSTMQRKTDFPIKNAVPEVEGEAESISSLCSQITNAFSTPEDPFSSAPMTKPVTVVAPQSPTFQANGTDSAFHVLAKPAHTALAPVAMPVRETNPWAHAPDAANKEIAATCSGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQASAAPLQPVLQPPPPTAISQPASPFQGNAFLTSQPVPVGVVPALQPAFVPAQSYPVANGMPYPAPNVPVVGITPSQMVANVFGTAGHPQAAHPHQSPSLVRQQTFPHYEASSATTSPFFKPPAQHLNGSAAFNGVDDGRLASADRHTEVPTGTCPVDPFEAQWAALENKSKQRTNPSPTNPFSSDLQKTFEIEL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P49757 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| Y15 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| R53 | Methylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| T102 | Phosphorylation | Q2M2I8 (AAK1) | Uniprot |
| R121 | Methylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| T190 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T246 | Phosphorylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Q14012 (CAMK1) | Uniprot |
| R278 | Methylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| S288 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S295 | Phosphorylation | Q14012 (CAMK1) | Uniprot |
| S361 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| T436 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| T561 | Phosphorylation | Uniprot | |
| T631 | Phosphorylation | Uniprot | |
| S634 | Phosphorylation | Uniprot |
Research Backgrounds
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
Phosphorylated on Ser-276 and Ser-295 by CaMK1.
Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Membrane>Peripheral membrane protein.
Interacts with CDH1 and TFAP2B (By similarity). Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis.
Research Fields
· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)
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