Product: Phospho-TOB1 (Ser164) Antibody
Catalog: AF4439
Description: Rabbit polyclonal antibody to Phospho-TOB1 (Ser164)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 38kDa; 38kD(Calculated).
Uniprot: P50616
RRID: AB_2844503

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-TOB1 (Ser164) Antibody detects endogenous levels of TOB1 only when phosphorylated at Ser164.
RRID:
AB_2844503
Cite Format: Affinity Biosciences Cat# AF4439, RRID:AB_2844503.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

APRO 6; APRO6; MGC104792; MGC34446; PIG 49; PIG49; Proliferation inducing gene 49; Protein Tob1; RP23 244C22.1; TOB 1; Tob 1 protein; TOB; TOB1; Tob1 protein; Transducer of ERBB 2 1; Transducer of erbB 2; Transducer of ERBB2 1; Transducer of erbB2; Trob; TROB 1; TROB1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P50616 TOB1_HUMAN:

Ubiquitous.

Sequence:
MQLEIQVALNFIISYLYNKLPRRRVNIFGEELERLLKKKYEGHWYPEKPYKGSGFRCIHIGEKVDPVIEQASKESGLDIDDVRGNLPQDLSVWIDPFEVSYQIGEKGPVKVLYVDDNNENGCELDKEIKNSFNPEAQVFMPISDPASSVSSSPSPPFGHSAAVSPTFMPRSTQPLTFTTATFAATKFGSTKMKNSGRSNKVARTSPINLGLNVNDLLKQKAISSSMHSLYGLGLGSQQQPQQQQQPAQPPPPPPPPQQQQQQKTSALSPNAKEFIFPNMQGQGSSTNGMFPGDSPLNLSPLQYSNAFDVFAAYGGLNEKSFVDGLNFSLNNMQYSNQQFQPVMAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50616 As Substrate

Site PTM Type Enzyme
Y40 Phosphorylation
K73 Ubiquitination
K110 Ubiquitination
K126 Ubiquitination
S152 Phosphorylation P45984 (MAPK9) , P28482 (MAPK1) , P27361 (MAPK3)
S154 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) , P45984 (MAPK9)
S164 Phosphorylation P45984 (MAPK9) , P28482 (MAPK1) , P27361 (MAPK3)
T204 Phosphorylation
S205 Phosphorylation
S268 Phosphorylation

Research Backgrounds

Function:

Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay.

PTMs:

Phosphorylated on Ser and Thr residues.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Only a small fraction localizes to the cytoplasm except in late S-phase where more than half of proteins become cytoplasmic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with ERBB2. Interacts with CNOT7. Interacts with CPEB3 (via C-terminal RNA-binding region); recruits CNOT7 to CPEB3 to form a ternary complex required for mRNA deadenylation and decay. Interacts with CNOT8. Interacts with CPEB4.

Family&Domains:

Belongs to the BTG family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.