Phospho-FRA1 (Ser265) Antibody - #AF4410

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Product: Phospho-FRA1 (Ser265) Antibody
Catalog: AF4410
Description: Rabbit polyclonal antibody to Phospho-FRA1 (Ser265)
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 43kDa; 29kD(Calculated).
Uniprot: P15407
RRID: AB_2844475

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-FRA1 (Ser265) Antibody detects endogenous levels of FRA1 only when phosphorylated at Ser265.
RRID:
AB_2844475
Cite Format: Affinity Biosciences Cat# AF4410, RRID:AB_2844475.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FOS L1; FOS like antigen 1; Fos related antigen 1; Fos-related antigen 1; FOSL 1; FOSL1; FOSL1 protein; FOSL1_HUMAN; FRA 1; FRA-1;

Immunogens

Immunogen:
Uniprot:

P15407(FOSL1_HUMAN) >>Visit HPA database.

Gene(ID):
FOSL1(8061)
Sequence:
MFRDFGEPGPSSGNGGGYGGPAQPPAAAQAAQQKFHLVPSINTMSGSQELQWMVQPHFLGPSSYPRPLTYPQYSPPQPRPGVIRALGPPPGVRRRPCEQISPEEEERRRVRRERNKLAAAKCRNRRKELTDFLQAETDKLEDEKSGLQREIEELQKQKERLELVLEAHRPICKIPEGAKEGDTGSTSGTSSPPAPCRPVPCISLSPGPVLEPEALHTPTLMTTPSLTPFTPSLVFTYPSTPEPCASAHRKSSSSSGDPSSDPLGSPTLLAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15407 As Substrate

Site PTM Type Enzyme
R66 Methylation
T69 Phosphorylation
S74 Phosphorylation
R79 Methylation
R84 Methylation
S101 Phosphorylation
K144 Ubiquitination
T183 Phosphorylation
S191 Phosphorylation
T217 Phosphorylation
T223 Phosphorylation Q04759 (PRKCQ)
T227 Phosphorylation
T230 Phosphorylation Q04759 (PRKCQ)
T240 Phosphorylation
S246 Phosphorylation
S251 Phosphorylation
S252 Phosphorylation
S253 Phosphorylation
S254 Phosphorylation
S265 Phosphorylation Q04759 (PRKCQ)
T267 Phosphorylation

Research Backgrounds

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer.

Family&Domains:

Belongs to the bZIP family. Fos subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

References

1). Glucocorticoids induce osteonecrosis of the femoral head in rats via PI3K/AKT/FOXO1 signaling pathway. PeerJ (PubMed: 35529482) [IF=2.7]

Application: WB    Species: Mice    Sample: MC3T3-E1 cells

Figure 4 Dex inhibited the activities of PI3K/AKT signaling pathways in MC3T3-E1 cells. (A–B) Western blotting was performed to analyze the expression levels of PI3K, p-PI3K, AKT, p-AKT, FOXO1, and p-FOXO1 in MC3T3-E1 cells after Dex treatment (200 µM, 48 h). Con: control group. All data in vivo and in vitro experiments are triplicate biological replicates. * P < 0.05, ** P < 0.01, *** P < 0.0001.
2). Glucocorticoids induce osteonecrosis of the femoral head in rats via PI3K/AKT/FOXO1 signaling pathway. PeerJ (PubMed: 35529482) [IF=2.7]

Application: WB    Species: Mice    Sample: MC3T3-E1 cells

Figure 4 Dex inhibited the activities of PI3K/AKT signaling pathways in MC3T3-E1 cells. (A–B) Western blotting was performed to analyze the expression levels of PI3K, p-PI3K, AKT, p-AKT, FOXO1, and p-FOXO1 in MC3T3-E1 cells after Dex treatment (200 µM, 48 h). Con: control group. All data in vivo and in vitro experiments are triplicate biological replicates. * P < 0.05, ** P < 0.01, *** P < 0.0001.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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