ROS Antibody - #DF10344
| Product: | ROS Antibody |
| Catalog: | DF10344 |
| Description: | Rabbit polyclonal antibody to ROS |
| Application: | WB |
| Reactivity: | Human, Rat |
| Prediction: | Pig, Horse, Sheep |
| Mol.Wt.: | 263KD; 264kD(Calculated). |
| Uniprot: | P08922 |
| RRID: | AB_2844351 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF10344, RRID:AB_2844351.
Fold/Unfold
c ros 1; c ros; c ros oncogene 1 receptor tyrosine kinase; c Ros receptor tyrosine kinase; c ros1; MCF 3; MCF3; Oncogene ROS; OTTHUMP00000017814; OTTHUMP00000017815; OTTHUMP00000040389; Proto oncogene c ros 1 protein; Proto oncogene c Ros; Proto oncogene tyrosine protein kinase ROS; Proto oncogene tyrosine protein kinase ROS precursor; Proto-oncogene c-Ros-1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c ros oncogene 1; Ros 1; ROS 1C; ROS; ROS proto oncogene 1 , receptor tyrosine kinase; ROS_HUMAN; ROS1; ROS1C; Transmembrane tyrosine specific protein kinase; v ros avian UR2 sarcoma virus oncogene homolog 1; v ros UR2 sarcoma virus oncogene homolog 1;
Immunogens
- P08922 ROS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKNIYCLIPKLVNFATLGCLWISVVQCTVLNSCLKSCVTNLGQQLDLGTPHNLSEPCIQGCHFWNSVDQKNCALKCRESCEVGCSSAEGAYEEEVLENADLPTAPFASSIGSHNMTLRWKSANFSGVKYIIQWKYAQLLGSWTYTKTVSRPSYVVKPLHPFTEYIFRVVWIFTAQLQLYSPPSPSYRTHPHGVPETAPLIRNIESSSPDTVEVSWDPPQFPGGPILGYNLRLISKNQKLDAGTQRTSFQFYSTLPNTIYRFSIAAVNEVGEGPEAESSITTSSSAVQQEEQWLFLSRKTSLRKRSLKHLVDEAHCLRLDAIYHNITGISVDVHQQIVYFSEGTLIWAKKAANMSDVSDLRIFYRGSGLISSISIDWLYQRMYFIMDELVCVCDLENCSNIEEITPPSISAPQKIVADSYNGYVFYLLRDGIYRADLPVPSGRCAEAVRIVESCTLKDFAIKPQAKRIIYFNDTAQVFMSTFLDGSASHLILPRIPFADVKSFACENNDFLVTDGKVIFQQDALSFNEFIVGCDLSHIEEFGFGNLVIFGSSSQLHPLPGRPQELSVLFGSHQALVQWKPPALAIGANVILISDIIELFELGPSAWQNWTYEVKVSTQDPPEVTHIFLNISGTMLNVPELQSAMKYKVSVRASSPKRPGPWSEPSVGTTLVPASEPPFIMAVKEDGLWSKPLNSFGPGEFLSSDIGNVSDMDWYNNSLYYSDTKGDVFVWLLNGTDISENYHLPSIAGAGALAFEWLGHFLYWAGKTYVIQRQSVLTGHTDIVTHVKLLVNDMVVDSVGGYLYWTTLYSVESTRLNGESSLVLQTQPWFSGKKVIALTLDLSDGLLYWLVQDSQCIHLYTAVLRGQSTGDTTITEFAAWSTSEISQNALMYYSGRLFWINGFRIITTQEIGQKTSVSVLEPARFNQFTIIQTSLKPLPGNFSFTPKVIPDSVQESSFRIEGNASSFQILWNGPPAVDWGVVFYSVEFSAHSKFLASEQHSLPVFTVEGLEPYALFNLSVTPYTYWGKGPKTSLSLRAPETVPSAPENPRIFILPSGKCCNKNEVVVEFRWNKPKHENGVLTKFEIFYNISNQSITNKTCEDWIAVNVTPSVMSFQLEGMSPRCFIAFQVRAFTSKGPGPYADVVKSTTSEINPFPHLITLLGNKIVFLDMDQNQVVWTFSAERVISAVCYTADNEMGYYAEGDSLFLLHLHNRSSSELFQDSLVFDITVITIDWISRHLYFALKESQNGMQVFDVDLEHKVKYPREVKIHNRNSTIISFSVYPLLSRLYWTEVSNFGYQMFYYSIISHTLHRILQPTATNQQNKRNQCSCNVTEFELSGAMAIDTSNLEKPLIYFAKAQEIWAMDLEGCQCWRVITVPAMLAGKTLVSLTVDGDLIYWIITAKDSTQIYQAKKGNGAIVSQVKALRSRHILAYSSVMQPFPDKAFLSLASDTVEPTILNATNTSLTIRLPLAKTNLTWYGITSPTPTYLVYYAEVNDRKNSSDLKYRILEFQDSIALIEDLQPFSTYMIQIAVKNYYSDPLEHLPPGKEIWGKTKNGVPEAVQLINTTVRSDTSLIISWRESHKPNGPKESVRYQLAISHLALIPETPLRQSEFPNGRLTLLVTRLSGGNIYVLKVLACHSEEMWCTESHPVTVEMFNTPEKPYSLVPENTSLQFNWKAPLNVNLIRFWVELQKWKYNEFYHVKTSCSQGPAYVCNITNLQPYTSYNVRVVVVYKTGENSTSLPESFKTKAGVPNKPGIPKLLEGSKNSIQWEKAEDNGCRITYYILEIRKSTSNNLQNQNLRWKMTFNGSCSSVCTWKSKNLKGIFQFRVVAANNLGFGEYSGISENIILVGDDFWIPETSFILTIIVGIFLVVTIPLTFVWHRRLKNQKSAKEGVTVLINEDKELAELRGLAAGVGLANACYAIHTLPTQEEIENLPAFPREKLTLRLLLGSGAFGEVYEGTAVDILGVGSGEIKVAVKTLKKGSTDQEKIEFLKEAHLMSKFNHPNILKQLGVCLLNEPQYIILELMEGGDLLTYLRKARMATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLMDGIFTTQSDVWSFGILIWEILTLGHQPYPAHSNLDVLNYVQTGGRLEPPRNCPDDLWNLMTQCWAQEPDQRPTFHRIQDQLQLFRNFFLNSIYKSRDEANNSGVINESFEGEDGDVICLNSDDIMPVALMETKNREGLNYMVLATECGQGEEKSEGPLGSQESESCGLRKEEKEPHADKDFCQEKQVAYCPSGKPEGLNYACLTHSGYGDGSD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P08922 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y135 | Phosphorylation | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| Y144 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| T196 | Phosphorylation | Uniprot | |
| Y800 | Phosphorylation | Uniprot | |
| Y802 | Phosphorylation | Uniprot | |
| Y807 | Phosphorylation | Uniprot | |
| S818 | Phosphorylation | Uniprot | |
| S1185 | Phosphorylation | Uniprot | |
| T1190 | Phosphorylation | Uniprot | |
| K1259 | Acetylation | Uniprot | |
| S1273 | Phosphorylation | Uniprot | |
| T1274 | Phosphorylation | Uniprot | |
| T1375 | Phosphorylation | Uniprot | |
| K1504 | Acetylation | Uniprot | |
| K1749 | Acetylation | Uniprot | |
| Y1923 | Phosphorylation | Uniprot | |
| T1927 | Phosphorylation | Uniprot | |
| T1930 | Phosphorylation | Uniprot | |
| K1944 | Acetylation | Uniprot | |
| S2002 | Phosphorylation | Uniprot | |
| Y2023 | Phosphorylation | Uniprot | |
| Y2069 | Phosphorylation | Uniprot | |
| Y2110 | Phosphorylation | Uniprot | |
| Y2114 | Phosphorylation | Uniprot | |
| Y2115 | Phosphorylation | Uniprot | |
| Y2173 | Phosphorylation | Uniprot | |
| Y2227 | Phosphorylation | Uniprot | |
| Y2274 | Phosphorylation | P08922 (ROS1) | Uniprot |
| Y2323 | Phosphorylation | Uniprot | |
| S2326 | Phosphorylation | Uniprot | |
| Y2334 | Phosphorylation | P08922 (ROS1) | Uniprot |
| T2338 | Phosphorylation | Uniprot | |
| Y2342 | Phosphorylation | Uniprot | |
| S2346 | Phosphorylation | Uniprot |
PTMs - P08922 As Enzyme
Research Backgrounds
Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2.
Phosphorylated. Probably autophosphorylates. Phosphorylation at Tyr-2274 is required for the interaction with PTPN6 that mediates ROS1 dephosphorylation (By similarity). Phosphorylation at Tyr-2274 stimulates the kinase activity and the activation of the ERK1 signaling cascade (By similarity). Phosphorylation at Tyr-2274 and/or Tyr-2334 recruits PTPN11.
Cell membrane>Single-pass type I membrane protein.
Expressed in brain. Expression is increased in primary gliomas.
Interacts with PTPN6 (via SH2 1 domain); the interaction is direct and promotes ROS1 dephosphorylation (By similarity). Interacts with PTPN11; may activate the PI3 kinase-mTOR signaling pathway. Interacts with VAV3; constitutive interaction mediating VAV3 phosphorylation.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
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