Product: NEK2 Antibody
Catalog: AF7557
Description: Rabbit polyclonal antibody to NEK2
Application: WB
Reactivity: Human, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 51kDa; 52kD(Calculated).
Uniprot: P51955
RRID: AB_2843921

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
NEK2 Antibody detects endogenous levels of total NEK2.
RRID:
AB_2843921
Cite Format: Affinity Biosciences Cat# AF7557, RRID:AB_2843921.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HSPK 21; HsPK21; NEK 2; NEK2; NEK2_HUMAN; NEK2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a) related kinase 2; NimA like protein kinase 1; NIMA related kinase 2; NimA related protein kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK 1; NLK1; Serine/threonine-protein kinase Nek2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P51955 NEK2_HUMAN:

Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.

Sequence:
MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Chicken
90
Xenopus
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51955 As Substrate

Site PTM Type Enzyme
K37 Ubiquitination
K63 Ubiquitination
K143 Ubiquitination
K152 Ubiquitination
K156 Ubiquitination
T170 Phosphorylation P51955 (NEK2)
S171 Phosphorylation P51955 (NEK2)
K174 Ubiquitination
T175 Phosphorylation P51955 (NEK2)
T179 Phosphorylation P51955 (NEK2)
S184 Phosphorylation
K227 Ubiquitination
S241 Phosphorylation P51955 (NEK2)
S261 Phosphorylation
K295 Ubiquitination
S296 Phosphorylation
S300 Phosphorylation
S304 Phosphorylation
K307 Ubiquitination
K309 Ubiquitination
K330 Ubiquitination
K344 Ubiquitination
K353 Ubiquitination
S356 Phosphorylation P51955 (NEK2) , Q13188 (STK3)
K359 Ubiquitination
K362 Ubiquitination
S365 Phosphorylation Q13188 (STK3)
S378 Phosphorylation
K381 Ubiquitination
K383 Ubiquitination
S387 Phosphorylation
S390 Phosphorylation
K391 Ubiquitination
S397 Phosphorylation P51955 (NEK2)
S400 Phosphorylation
S402 Phosphorylation P51955 (NEK2)
T405 Phosphorylation
S406 Phosphorylation Q13188 (STK3)
K407 Ubiquitination
S408 Phosphorylation
S428 Phosphorylation P51955 (NEK2)
K432 Ubiquitination
K437 Acetylation
K437 Ubiquitination
S438 Phosphorylation Q13188 (STK3)

PTMs - P51955 As Enzyme

Substrate Site Source
O14777 (NDC80) S165 Uniprot
O15360 (FANCA) T351 Uniprot
P06748 (NPM1) S70 Uniprot
P06748 (NPM1) S88 Uniprot
P36873 (PPP1CC) T307 Uniprot
P36873 (PPP1CC) T318 Uniprot
P51955 (NEK2) T170 Uniprot
P51955 (NEK2) S171 Uniprot
P51955 (NEK2) T175 Uniprot
P51955 (NEK2) T179 Uniprot
P51955 (NEK2) S241 Uniprot
P51955 (NEK2) S356 Uniprot
P51955 (NEK2) S397 Uniprot
P51955 (NEK2) S402 Uniprot
P51955 (NEK2) S428 Uniprot
Q5FBB7 (SGO1) S14 Uniprot
Q5FBB7 (SGO1) S507 Uniprot
Q8N137 (CNTROB) T35 Uniprot
Q8N137 (CNTROB) S36 Uniprot
Q8N137 (CNTROB) S41 Uniprot
Q8N137 (CNTROB) S45 Uniprot
Q9BV73 (CEP250) S2392 Uniprot
Q9BV73 (CEP250) S2394 Uniprot
Q9BV73 (CEP250) S2417 Uniprot
Q9BV73 (CEP250) S2421 Uniprot
Q9H2D6-5 (TRIOBP) T221 Uniprot

Research Backgrounds

Function:

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Involved in the regulation of centrosome disjunction.

Phosphorylates and activates NEK11 in G1/S-arrested cells.

Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.

PTMs:

Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.

Subcellular Location:

Nucleus. Nucleus>Nucleolus. Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole. Chromosome>Centromere>Kinetochore. Chromosome>Centromere.
Note: STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGO1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.

Cytoplasm.
Note: Predominantly cytoplasmic.

Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Predominantly nuclear.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.

Subunit Structure:

Isoform 1, isoform 2 and isoform 4 form homo- and heterodimers. Interacts with NECAB3 and HMGA2 (By similarity). Isoform 1 interacts with CDC20, CTNB1, MAD1L1, MAPK, NEK11, NPM1, NDC80, PCNT and SGO1. Isoform 1 interacts with STK3/MST2 (via SARAH domain) and SAV1 (via SARAH domain). Isoform 1 and isoform 2 interact with MAD2L1. Isoform 1 and isoform 4 interact with PPP1CA and PPP1CC. Interacts with CEP68; the interaction leads to phosphorylation of CEP68. Interacts with CNTLN; the interaction leads to phosphorylation of CNTLN. Isoform 1 interacts with CEP85.

Family&Domains:

The leucine-zipper domain is required for its dimerization and activation.

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Restrictive clause

 

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