POU2F2 Antibody | Affinity Biosciences

IHC
IF/ICC
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AF0225 at 1/100 staining Rat testis tissue by IHC-P.

Product:	POU2F2 Antibody
Catalog:	AF0225
Description:	Rabbit polyclonal antibody to POU2F2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	52kDa; 51kD(Calculated).
Uniprot:	P09086
RRID:	AB_2833400

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC: 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Bovine(88%), Horse(93%), Sheep(100%), Rabbit(94%), Dog(100%)

Clonality:

Polyclonal

Specificity:

OCT2 Antibody detects endogenous levels of total OCT2.

RRID:

AB_2833400
Cite Format: Affinity Biosciences Cat# AF0225, RRID:AB_2833400.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

class 2; Lymphoid restricted immunoglobulin octamer binding protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; NF A2; Oct 2; Oct-2; Octamer binding transcription factor 2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; OTF2; PO2F2_HUMAN; POU domain; POU domain class 2 transcription factor 2; POU2F2; transcription factor 2;

Immunogens

Immunogen:

Uniprot:

P09086(PO2F2_HUMAN) >>Visit HPA database.

Gene(ID):

POU2F2(5452)

Expression:

P09086 PO2F2_HUMAN:

Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.

Description:

POU2F2 Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. Belongs to the POU transcription factor family.

Sequence:

P09086 PO2F2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVHSSMGAPEIRMSKPLEAEKQGLDSPSEHTDTERNGPDTNHQNPQNKTSPFSVSPTGPSTKIKAEDPSGDSAPAAPLPPQPAQPHLPQAQLMLTGSQLAGDIQQLLQLQQLVLVPGHHLQPPAQFLLPQAQQSQPGLLPTPNLFQLPQQTQGALLTSQPRAGLPTQAVTRPTLPDPHLSHPQPPKCLEPPSHPEEPSDLEELEQFARTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAETMSVDSSLPSPNQLSSPSLGFDGLPGRRRKKRTSIETNVRFALEKSFLANQKPTSEEILLIAEQLHMEKEVIRVWFCNRRQKEKRINPCSAAPMLPSPGKPASYSPHMVTPQGGAGTLPLSQASSSLSTTVTTLSSAVGTLHPSRTAGGGGGGGGAAPPLNSIPSVTPPPPATTNSTNPSPQGSHSAIGLSGLNPSTGPGLWWNPAPYQP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Rabbit

Horse

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09086 As Substrate

P09086

Site	PTM Type	Source
S4	Phosphorylation	Uniprot
S5	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
S26	Phosphorylation	Uniprot
S53	Phosphorylation	Uniprot
S55	Phosphorylation	Uniprot
S192	O-Glycosylation	Uniprot
K216	Ubiquitination	Uniprot
K230	Ubiquitination	Uniprot
S250	Phosphorylation	Uniprot
S272	O-Glycosylation	Uniprot
S275	O-Glycosylation	Uniprot
T302	O-Glycosylation	Uniprot
T306	Phosphorylation	Uniprot
R309	Methylation	Uniprot
S324	O-Glycosylation	Uniprot
S372	O-Glycosylation	Uniprot
S390	O-Glycosylation	Uniprot
S395	O-Glycosylation	Uniprot

Research Backgrounds

P09086_PO2F2_HUMAN

Function:

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.

Subunit Structure:

Interacts with NR3C1, AR and PGR.

Family&Domains:

Belongs to the POU transcription factor family. Class-2 subfamily.

References

1). Caffeic acid phenethyl ester alleviated hypouricemia in hyperuricemic mice through inhibiting XOD and up-regulating OAT3. PHYTOMEDICINE, 2022 (PubMed: 35714456) [IF=7.9]

2). Hypouricaemic and nephroprotective effects of Poria cocos in hyperuricemic mice by up-regulating ATP-binding cassette super-family G member 2. PHARMACEUTICAL BIOLOGY, 2021 (PubMed: 33651969) [IF=3.8]

Application: WB Species: Mice Sample: kidney tissues

Figure 4. Effects of PCE and PCW on renal ABCG2, OAT3, OAT1 and OCT2 protein expression detected by Western blot: immunoreactive bands (a) and densitometries (b,c,d and –e, expressed as mean ± SD; n n ¼ 3). p < 0.05, p < 0.01 versus the normal control; #p < 0.05, ##p < 0.01 versus the hyperuricemic control; p < 0.01 versus the allopurinol control.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

POU2F2 Antibody - #AF0225