Product: Phospho-JunD (Ser255) Antibody
Catalog: AF3200
Description: Rabbit polyclonal antibody to Phospho-JunD (Ser255)
Application: WB IHC IF/ICC IP
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Dog, Chicken
Mol.Wt.: 38kDa; 35kD(Calculated).
Uniprot: P17535
RRID: AB_2834632

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-JunD (Ser255) Antibody detects endogenous levels of JunD only when phosphorylated at Serine 255.
RRID:
AB_2834632
Cite Format: Affinity Biosciences Cat# AF3200, RRID:AB_2834632.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activator protein 1; AP 1; AP1; Jun D; jun D proto oncogene; Jund; JunD FL isoform; JUND_HUMAN; Transcription factor jun D; Transcription factor jun-D;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
JunD Transcription factor binding AP-1 sites. Binds DNA as a dimer. Interacts with MEN1; this interaction represses transcriptional activation. Belongs to the bZIP family. Jun subfamily.
Sequence:
METPFYGDEALSGLGGGASGSGGSFASPGRLFPGAPPTAAAGSMMKKDALTLSLSEQVAAALKPAAAPPPTPLRADGAPSAAPPDGLLASPDLGLLKLASPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFVKALEDLHKQNQLGAGAAAAAAAAAAGGPSGTATGSAPPGELAPAAAAPEAPVYANLSSYAGGAGGAGGAATVAFAAEPVPFPPPPPPGALGPPRLAALKDEPQTVPDVPSFGESPPLSPIDMDTQERIKAERKRLRNRIAASKCRKRKLERISRLEEKVKTLKSQNTELASTASLLREQVAQLKQKVLSHVNSGCQLLPQHQVPAY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Chicken
100
Zebrafish
89
Xenopus
73
Horse
0
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17535 As Substrate

Site PTM Type Enzyme
S27 Phosphorylation
T38 Phosphorylation
S43 Phosphorylation
T71 Phosphorylation
S90 Phosphorylation P45983 (MAPK8)
K97 Ubiquitination
S100 Phosphorylation P45983 (MAPK8) , P27361 (MAPK3) , P28482 (MAPK1)
T117 Phosphorylation P45983 (MAPK8)
K127 Ubiquitination
S131 Phosphorylation
K142 Ubiquitination
K240 Sumoylation
T245 Phosphorylation
S251 Phosphorylation
S255 Phosphorylation
S259 Phosphorylation
T265 Phosphorylation
S294 Phosphorylation
S305 Phosphorylation
T308 Phosphorylation
S312 Phosphorylation
T313 Phosphorylation
S315 Phosphorylation
K325 Ubiquitination
K327 Ubiquitination

Research Backgrounds

Function:

Transcription factor binding AP-1 sites.

PTMs:

Phosphorylated by MAP kinases MAPK8 and MAPK10; phosphorylation is inhibited in the presence of MEN1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds DNA as a dimer (By similarity). Interacts (via MBM motif) with MEN1; this interaction represses transcriptional activation. Interacts with MAPK10; this interaction is inhibited in the presence of MEN1.

Family&Domains:

Belongs to the bZIP family. Jun subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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