Phospho-BCLAF1 (Ser512) Antibody - #AF8615

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Product: Phospho-BCLAF1 (Ser512) Antibody
Catalog: AF8615
Description: Rabbit polyclonal antibody to Phospho-BCLAF1 (Ser512)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 106 KD; 106kD(Calculated).
Uniprot: Q9NYF8
RRID: AB_2843467

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 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
Phospho-BCLAF1 (Ser512) Antibody detects endogenous levels of BCLAF1 only when phosphorylated at S512.
RRID:
AB_2843467
Cite Format: Affinity Biosciences Cat# AF8615, RRID:AB_2843467.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Bcl 2 associated transcription factor; Bcl-2-associated transcription factor 1; BCL2 associated transcription factor 1; BCLAF1; BCLF1_HUMAN; bK211L9.1; Btf; KIAA0164;

Immunogens

Immunogen:
Uniprot:

Q9NYF8(BCLF1_HUMAN) >>Visit HPA database.

Gene(ID):
BCLAF1(9774)
Expression:
Q9NYF8 BCLF1_HUMAN:

Ubiquitous.

Description:
Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
Sequence:
MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRSRDRMYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSSATSGDIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNGSSRYSPSQNSPIHHIPSRRSPAKTIAPQNAPRDESRGRSSFYPDGGDQETAKTGKFLKRFTDEESRVFLLDRGNTRDKEASKEKGSEKGRAEGEWEDQEALDYFSDKESGKQKFNDSEGDDTEETEDYRQFRKSVLADQGKSFATASHRNTEEEGLKYKSKVSLKGNRESDGFREEKNYKLKETGYVVERPSTTKDKHKEEDKNSERITVKKETQSPEQVKSEKLKDLFDYSPPLHKNLDAREKSTFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQASKSTSESFIQHIVSLVHHVKEQYFKSAAMTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSTLRKHTRLAGEERVFKEENQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKSYKDDSKHKREQDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKEYSGFAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYTPKSKKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDEEETMENNEEKKDRRKEEKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NYF8 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
Y34 Phosphorylation
S36 Phosphorylation
S38 Phosphorylation
S44 Phosphorylation
S46 Phosphorylation
S48 Phosphorylation
S54 Phosphorylation
R55 Methylation
R76 Methylation
R78 Methylation
Y80 Phosphorylation P12931 (SRC)
Y81 Phosphorylation P12931 (SRC)
R87 Methylation
Y88 Phosphorylation
R90 Methylation
Y93 Phosphorylation P12931 (SRC)
R94 Methylation
S102 Phosphorylation
S104 Phosphorylation
S119 Phosphorylation
S121 Phosphorylation
S122 Phosphorylation
S125 Phosphorylation
S132 Phosphorylation
S135 Phosphorylation
S136 Phosphorylation
S138 Phosphorylation
S141 Phosphorylation
S142 Phosphorylation
S146 Phosphorylation
S148 Phosphorylation
Y150 Phosphorylation P12931 (SRC)
S151 Phosphorylation
K152 Acetylation
S153 Phosphorylation
S156 Phosphorylation
S161 Phosphorylation
K168 Sumoylation
S177 Phosphorylation
S181 Phosphorylation
S183 Phosphorylation
K188 Ubiquitination
T190 Phosphorylation
S196 Phosphorylation
S198 Phosphorylation
S206 Phosphorylation
T208 Phosphorylation
S209 Phosphorylation
S217 Phosphorylation
Y219 Phosphorylation
S222 Phosphorylation
S225 Phosphorylation
S228 Phosphorylation
S230 Phosphorylation
T234 Phosphorylation
T257 Phosphorylation
S259 Phosphorylation
S262 Phosphorylation
S264 Phosphorylation
S268 Phosphorylation
S272 Phosphorylation
S274 Phosphorylation
S276 Phosphorylation
S281 Phosphorylation
S282 Phosphorylation
Y284 Phosphorylation P12931 (SRC)
S285 Phosphorylation
S287 Phosphorylation
S290 Phosphorylation
S297 Phosphorylation
S300 Phosphorylation
S315 Phosphorylation
S319 Phosphorylation
S320 Phosphorylation
T330 Phosphorylation
K332 Acetylation
K332 Sumoylation
K332 Ubiquitination
K335 Acetylation
K335 Sumoylation
T341 Phosphorylation
S345 Phosphorylation
R352 Methylation
T355 Phosphorylation
Y383 Phosphorylation
S385 Phosphorylation
S389 Phosphorylation
S397 Phosphorylation
T402 Phosphorylation
T405 Phosphorylation
Y408 Phosphorylation P12931 (SRC)
K413 Sumoylation
K413 Ubiquitination
S414 Phosphorylation
K421 Acetylation
K421 Sumoylation
K421 Ubiquitination
S422 Phosphorylation
T425 Phosphorylation
S427 Phosphorylation
R429 Methylation
T431 Phosphorylation
K437 Acetylation
K437 Methylation
K437 Sumoylation
Y438 Phosphorylation
K439 Acetylation
S443 Phosphorylation
K445 Methylation
S450 Phosphorylation
Y459 Phosphorylation
K462 Sumoylation
K462 Ubiquitination
T464 Phosphorylation
Y466 Phosphorylation
S472 Phosphorylation
T473 Phosphorylation
T474 Phosphorylation
K475 Ubiquitination
S485 Phosphorylation
T489 Phosphorylation
K491 Sumoylation
T494 Phosphorylation
S496 Phosphorylation
K501 Sumoylation
S502 Phosphorylation
Y511 Phosphorylation
S512 Phosphorylation
K517 Ubiquitination
S525 Phosphorylation
T526 Phosphorylation
S531 Phosphorylation P49841 (GSK3B)
K536 Sumoylation
K536 Ubiquitination
S540 Phosphorylation
S542 Phosphorylation
K548 Sumoylation
K550 Sumoylation
S559 Phosphorylation
S564 Phosphorylation
T566 Phosphorylation
K567 Methylation
K567 Ubiquitination
S573 Phosphorylation
T574 Phosphorylation
S578 Phosphorylation
K580 Sumoylation
K593 Ubiquitination
S604 Phosphorylation
K622 Methylation
K622 Ubiquitination
K637 Acetylation
K637 Ubiquitination
S648 Phosphorylation
S658 Phosphorylation
S660 Phosphorylation
T661 Phosphorylation
K676 Sumoylation
K684 Acetylation
K685 Acetylation
S690 Phosphorylation
S711 Phosphorylation
S714 Phosphorylation
S718 Phosphorylation
S720 Phosphorylation
T726 Phosphorylation
Y730 Phosphorylation
K731 Sumoylation
Y733 Phosphorylation
S749 Phosphorylation
S751 Phosphorylation
S753 Phosphorylation
S755 Phosphorylation
S757 Phosphorylation
S759 Phosphorylation
S760 Phosphorylation
S762 Phosphorylation
S763 Phosphorylation
K778 Methylation
K778 Sumoylation
Y786 Phosphorylation
S793 Phosphorylation
R794 Methylation
R796 Methylation
R809 Methylation
T817 Phosphorylation
T824 Phosphorylation
T825 Phosphorylation
K831 Sumoylation
Y839 Phosphorylation
T840 Phosphorylation
K842 Ubiquitination
K845 Sumoylation
K845 Ubiquitination
Y846 Phosphorylation
Y858 Phosphorylation
S881 Phosphorylation
S883 Phosphorylation
S884 Phosphorylation
K891 Acetylation
K891 Ubiquitination

Research Backgrounds

Function:

Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.

PTMs:

Citrullinated by PADI4.

Subcellular Location:

Cytoplasm. Nucleus. Nucleus speckle. Nucleus>Nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. Component of the SNARP complex which consists at least of SNIP1, SNW1, THRAP3, BCLAF1 and PNN. Component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, KIAA1429, RBM15, BCLAF1 and THRAP3.

Family&Domains:

Belongs to the BCLAF1/THRAP3 family.

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