Product: Phospho-ketohexokinase (Ser80) Antibody-Isoform A
Catalog: AF8612
Source: Rabbit
Application: WB, ELISA(peptide)
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep
Mol.Wt.: 33 kD; 33kD(Calculated).
Uniprot: P50053
RRID: AB_2843461

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse
Prediction:
Bovine(88%), Horse(100%), Sheep(88%)
Clonality:
Polyclonal
Specificity:
Phospho-ketohexokinase (Ser80) Antibody-Isoform A detects endogenous levels of ketohexokinase only when phosphorylated at S80.
RRID:
AB_2843461
Cite Format: Affinity Biosciences Cat# AF8612, RRID:AB_2843461.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.7.1.3; Fructokinase; Hepatic fructokinase; Ketohexokinase; Ketohexokinase isoform a; KHK; KHK_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human Ketohexokinase(Isoform A) around the phosphorylation site of S80.

Uniprot:
Gene(ID):
Expression:
P50053 KHK_HUMAN:

Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.

Description:
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Sequence:
MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSMAPGHVADFLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
88
Sheep
88
Pig
63
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50053 As Substrate

Site PTM Type Enzyme
S90 Phosphorylation
S116 Phosphorylation
K134 Ubiquitination
K148 Ubiquitination
K197 Ubiquitination

Research Backgrounds

Function:

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.

Tissue Specificity:

Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the carbohydrate kinase PfkB family.

Research Fields

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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