Phospho-PFKP (Tyr52) Antibody - #AF8610

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Product: Phospho-PFKP (Tyr52) Antibody
Catalog: AF8610
Description: Rabbit polyclonal antibody to Phospho-PFKP (Tyr52)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 84 KD; 86kD(Calculated).
Uniprot: Q01813
RRID: AB_2843460

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:100-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-PFKP (Tyr52) Antibody detects endogenous levels of PFKP only when phosphorylated at Y52.
RRID:
AB_2843460
Cite Format: Affinity Biosciences Cat# AF8610, RRID:AB_2843460.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1200015H23Rik; 6 phosphofructokinase type C; 6 phosphofructokinase, platelet type; 6-phosphofructokinase; 6-phosphofructokinase type C; 9330125N24Rik; FLJ40226; K6PP; K6PP_HUMAN; MGC105718; PFK C; PFK, fibroblast type; PFK-C; PFKF; PFKP; Phosphofructo 1 kinase isozyme C; Phosphofructo-1-kinase isozyme C; Phosphofructokinase 1; Phosphofructokinase platelet; Phosphohexokinase; platelet type;

Immunogens

Immunogen:
Uniprot:

Q01813(PFKAP_HUMAN) >>Visit HPA database.

Gene(ID):
PFKP(5214)
Description:
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Sequence:
MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPDTPACVVSLNGNHAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDDQIPKTNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVSDSGQLEHVQPWSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01813 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S6 Phosphorylation
K10 Ubiquitination
S12 Phosphorylation
K15 Ubiquitination
S21 Phosphorylation
K25 Ubiquitination
Y52 Phosphorylation
Y64 Phosphorylation
K109 Acetylation
K109 Methylation
K109 Ubiquitination
K139 Ubiquitination
S142 Phosphorylation
K156 Acetylation
K156 Ubiquitination
T211 Phosphorylation
K287 Ubiquitination
K289 Ubiquitination
R310 Methylation
T313 Phosphorylation
R319 Methylation
S386 Phosphorylation
T393 Phosphorylation
K395 Acetylation
K395 Ubiquitination
K400 Ubiquitination
C411 S-Nitrosylation
Y447 Phosphorylation
K455 Ubiquitination
K459 Ubiquitination
K480 Acetylation
K480 Ubiquitination
K486 Acetylation
K486 Ubiquitination
Y487 Phosphorylation
K567 Ubiquitination
K574 Acetylation
Y586 Phosphorylation
K625 Ubiquitination
K627 Ubiquitination
Y645 Phosphorylation
Y651 Phosphorylation
Y654 Phosphorylation
K666 Ubiquitination
K688 Acetylation
K688 Ubiquitination
K700 Acetylation
K724 Ubiquitination
K736 Acetylation
K736 Methylation
K736 Ubiquitination
K737 Methylation
K737 Ubiquitination
K747 Acetylation
K747 Ubiquitination
K753 Ubiquitination
K759 Ubiquitination
K765 Ubiquitination
S783 Phosphorylation

Research Backgrounds

Function:

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

PTMs:

GlcNAcylation decreases enzyme activity.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homo- and heterotetramers (By similarity). Phosphofructokinase (PFK) enzyme functions as a tetramer composed of different combinations of 3 types of subunits, called PFKM (where M stands for Muscle), PFKL (Liver) and PFKP (Platelet). The composition of the PFK tetramer differs according to the tissue type it is present in. In muscles, it is composed of 4 PFKM subunits (also called M4). In the liver, the predominant form is a tetramer of PFKL subunits (L4). In erythrocytes, both PFKM and PFKL subunits randomly tetramerize to form M4, L4 and other combinations (ML3, M2L2, M3L). In platelets, brain and fibroblasts, PFK contains a higher proportion of PFKP subunits. The kinetic and regulatory properties of the tetrameric enzyme are dependent on the subunit composition, hence can vary across tissues (Probable).

Family&Domains:

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.

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