Product: Phospho-CKB (Ser199) Antibody
Catalog: AF8602
Description: Rabbit polyclonal antibody to Phospho-CKB (Ser199)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: P12277
RRID: AB_2843453

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 100ul $350 In stock
 200ul $450 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-CKB (Ser199) Antibody detects endogenous levels of CKB only when phosphorylated at S199.
RRID:
AB_2843453
Cite Format: Affinity Biosciences Cat# AF8602, RRID:AB_2843453.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B CK; B-CK; BB-CK; BCK; Brain creatine kinase; Ckb; CKBB; Creatine kinase B; Creatine kinase B chain; Creatine kinase B type; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine kinase brain type; Creatine phosphokinase BB; Epididymis luminal protein 211; Epididymis secretory protein Li 29; HEL 211; HEL S 29; KCRB_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence:
MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGYKPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVEALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12277 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S6 Phosphorylation
K11 Ubiquitination
R13 Methylation
S24 Phosphorylation
K32 Ubiquitination
T35 Phosphorylation
Y39 Phosphorylation
R43 Methylation
K45 Ubiquitination
Y82 Phosphorylation
K86 Ubiquitination
R96 Methylation
K101 Ubiquitination
K107 Ubiquitination
T108 Phosphorylation
Y125 Phosphorylation
S128 Phosphorylation
T133 Phosphorylation
S136 Phosphorylation
R138 Methylation
K156 Ubiquitination
S164 Phosphorylation
R172 Methylation
Y173 Phosphorylation
Y174 Phosphorylation
S178 Phosphorylation
K196 Ubiquitination
S199 Phosphorylation
R215 Methylation
K223 Sumoylation
K223 Ubiquitination
K242 Ubiquitination
K247 Ubiquitination
C254 S-Nitrosylation
T255 Phosphorylation
K265 Ubiquitination
K267 Acetylation
Y269 Phosphorylation
Y279 Phosphorylation
S285 Phosphorylation
K298 Ubiquitination
K304 Ubiquitination
K307 Sumoylation
K307 Ubiquitination
S309 Phosphorylation
K313 Acetylation
K313 Ubiquitination
R320 Methylation
T322 Phosphorylation
T327 Phosphorylation
S337 Phosphorylation
K381 Ubiquitination

Research Backgrounds

Function:

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Family&Domains:

Belongs to the ATP:guanido phosphotransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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