Price Size
$150 50ul
$250 100ul
$350 200ul
$1200 1ml

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  • Product Name
    Flag-Tag Antibody
  • Catalog No.
    T0053
  • RRID
    AB_2843447
  • Source
    Rabbit
  • Application
    WB,IF/ICC,IP,ELISA(peptide)
  • Reactivity
    All
  • Concentration
    1mg/ml

Product Information

Alternative Names:Expand▼

DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG tag antibody

Applications:

WB: 1:1000-1:30000, IF/ICC: 1:200, IP 1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

All

Source:

Rabbit

Purification:

Affinity-chromatography.

Specificity:

The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.

RRID:

AB_2843447
Please cite this product as: Affinity Biosciences Cat# T0053, RRID:AB_2843447.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthetic peptide DYKDDDDK coupled to KLH.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

Subcellular Location:

Endoplasmic reticulum membrane. Microsome membrane.

Description:

FLAG tag polyclonal antibody is part of the series of Tag antibodies, the excellent quality in the research. FLAG tag antibody is a highly sensitive and affinity PAB applicable to FLAG-tagged fusion protein detection. FLAG tag antibody can detect FLAG tags in internal, C-terminal, or N-terminal recombinant proteins.

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Catalog Number :

T0053-BP
(Blocking peptide available as T0053-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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