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  • Product Name
    Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated
  • Catalog No.
    S0006
  • Source
    Goat
  • Application
    WB,IF/ICC,FCM
  • Reactivity
    Rb

Product Information

Applications:

IF/ICC 1:100 – 1:500

Reactivity:

Rabbit

Source:

Goat

Clonality:

Polyclonal

Purification:

Affinity-chromatography.

Storage Condition and Buffer:

0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6. Store at -20℃,avoid repeated thawing and freezing.

Immunogen Information

Immunogen:

The antiserum was developed in goat using the Rabbit IgG as the immunogen.

Molecular Weight:

Observed Mol.Wt.: Not available.
Predicted Mol.Wt.: Not available.

TLR4 Ab staining U-2 OS cells by IF/ICC.The secondary antibody (red) was Alexa Fluor® 594 goat anti-rabbit IgG (H+L)(#S0006 ).

Reference Citations:

1). Yuan H et al. Osthole alleviated diabetic neuropathic pain mediated by the P2X4 receptor in dorsal root ganglia. Brain Res Bull 2018 Aug 14 (PubMed: 30118750)

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Catalog Number :

S0006-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

IMPORTANT: For western blots, incubate membrane with diluted secondary antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 37°C, 2H.