HC-II Antibody | Affinity Biosciences

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Product:	HC-II Antibody
Catalog:	AF9084
Description:	Rabbit polyclonal antibody to HC-II
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Rabbit, Dog
Mol.Wt.:	57kDa; 57kD(Calculated).
Uniprot:	P05546
RRID:	AB_2843275

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(88%), Horse(100%), Rabbit(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

HC-II Antibody detects endogenous levels of total HC-II.

RRID:

AB_2843275
Cite Format: Affinity Biosciences Cat# AF9084, RRID:AB_2843275.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

D22S673; HC II; HC-II; HC2; HCF2; HEP2_HUMAN; Heparin cofactor 2; Heparin cofactor II; HLS2; Leuserpin 2; LS2; Protease inhibitor leuserpin 2; Protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1; SERPIND1; THPH10;

Immunogens

Immunogen:

Uniprot:

P05546(HEP2_HUMAN) >>Visit HPA database.

Gene(ID):

SERPIND1(3053)

Expression:

P05546 HEP2_HUMAN:

Expressed predominantly in liver. Also present in plasma.

Sequence:

P05546 HEP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKHSLNALLIFLIITSAWGGSKGPLDQLEKGGETAQSADPQWEQLNNKNLSMPLLPADFHKENTVTNDWIPEGEEDDDYLDLEKIFSEDDDYIDIVDSLSVSPTDSDVSAGNILQLFHGKSRIQRLNILNAKFAFNLYRVLKDQVNTFDNIFIAPVGISTAMGMISLGLKGETHEQVHSILHFKDFVNASSKYEITTIHNLFRKLTHRLFRRNFGYTLRSVNDLYIQKQFPILLDFKTKVREYYFAEAQIADFSDPAFISKTNNHIMKLTKGLIKDALENIDPATQMMILNCIYFKGSWVNKFPVEMTHNHNFRLNEREVVKVSMMQTKGNFLAANDQELDCDILQLEYVGGISMLIVVPHKMSGMKTLEAQLTPRVVERWQKSMTNRTREVLLPKFKLEKNYNLVESLKLMGIRMLFDKNGNMAGISDQRIAIDLFKHQGTITVNEEGTQATTVTTVGFMPLSTQVRFTVDRPFLFLIYEHRTSCLLFMGRVANPSRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Bovine

Rabbit

Zebrafish

Sheep

Pig

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05546 As Substrate

P05546

Site	PTM Type	Source
S37	Phosphorylation	Uniprot
N49	N-Glycosylation	Uniprot
T66	Phosphorylation	Uniprot
Y79	Phosphorylation	Uniprot
Y138	Phosphorylation	Uniprot
N188	N-Glycosylation	Uniprot
N387	N-Glycosylation	Uniprot

Research Backgrounds

P05546_HEP2_HUMAN

Function:

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

PTMs:

Phosphorylated by FAM20C in the extracellular medium.

Tissue Specificity:

Expressed predominantly in liver. Also present in plasma.

Family&Domains:

The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.

Belongs to the serpin family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). Genistein treatment duration effects biomarkers of cell motility in human prostate. PLoS One (PubMed: 30917169) [IF=3.7]

Application: WB Species: human Sample: human prostate cell

S5 Fig. | Effect of BASP1 and HCF2 knockdown and overexpression on respective transcript and protein levels.E. comparison of BASP1 and HCF2 protein expression in native cell lines measured by Western blot.

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HC-II Antibody - #AF9084