Product: Phospho-Ezrin (Tyr146) Antibody
Catalog: AF3175
Description: Rabbit polyclonal antibody to Phospho-Ezrin (Tyr146)
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 69kDa; 69kD(Calculated).
Uniprot: P15311
RRID: AB_2834607

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Horse(83%), Rabbit(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
Phospho-Ezrin (Tyr146) Antibody detects endogenous levels of Ezrin only when phosphorylated at Tyrosine 146.
RRID:
AB_2834607
Cite Format: Affinity Biosciences Cat# AF3175, RRID:AB_2834607.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Villin 2 ezrin; CVIL; CVL; Cytovillin 2; Cytovillin; DKFZp762H157; Epididymis secretory protein Li 105; EZR; EZRI_HUMAN; Ezrin; FLJ26216; HEL S 105; MGC1584; p81; VIL 2; VIL2; Villin 2 (ezrin); Villin 2; Villin-2; Villin2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P15311 EZRI_HUMAN:

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

Description:
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton.
Sequence:
MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
92
Pig
83
Horse
83
Rabbit
83
Bovine
75
Xenopus
75
Chicken
75
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15311 As Substrate

Site PTM Type Enzyme
K3 Ubiquitination
K35 Acetylation
K35 Ubiquitination
T57 Phosphorylation
K60 Acetylation
K64 Ubiquitination
S66 Phosphorylation P17612 (PRKACA)
K72 Ubiquitination
K79 Acetylation
K79 Ubiquitination
K83 Ubiquitination
K107 Ubiquitination
S112 Phosphorylation
Y116 Phosphorylation
Y137 Phosphorylation
K139 Acetylation
K139 Ubiquitination
K143 Ubiquitination
S144 Phosphorylation
Y146 Phosphorylation P00533 (EGFR) , P16591 (FER) , P12931 (SRC) , P06239 (LCK)
S149 Phosphorylation
K162 Ubiquitination
R171 Methylation
K184 Acetylation
K184 Ubiquitination
Y191 Phosphorylation
Y201 Phosphorylation
Y205 Phosphorylation
K209 Ubiquitination
K211 Ubiquitination
K230 Sumoylation
K230 Ubiquitination
K233 Sumoylation
K233 Ubiquitination
T235 Phosphorylation Q00535 (CDK5)
K237 Ubiquitination
S243 Phosphorylation
S249 Phosphorylation
K253 Acetylation
K253 Ubiquitination
K254 Ubiquitination
K258 Acetylation
K258 Ubiquitination
K262 Acetylation
K262 Sumoylation
K262 Ubiquitination
K263 Acetylation
K263 Ubiquitination
Y270 Phosphorylation
R273 Methylation
Y291 Phosphorylation
K296 Ubiquitination
T299 Phosphorylation
K306 Methylation
K306 Ubiquitination
T332 Phosphorylation
K337 Ubiquitination
K344 Ubiquitination
Y354 Phosphorylation P16591 (FER) , P00533 (EGFR)
K357 Sumoylation
K357 Ubiquitination
S366 Phosphorylation
R393 Methylation
K412 Acetylation
K412 Ubiquitination
S413 Phosphorylation
Y424 Phosphorylation
T425 Phosphorylation
K427 Ubiquitination
R435 Methylation
K438 Ubiquitination
K450 Ubiquitination
K458 Ubiquitination
T468 Phosphorylation
Y478 Phosphorylation P12931 (SRC) , P16591 (FER)
S482 Phosphorylation
Y483 Phosphorylation
S488 Phosphorylation
T497 Phosphorylation
Y499 Phosphorylation
K523 Ubiquitination
S535 Phosphorylation
S536 Phosphorylation
S539 Phosphorylation
T548 Phosphorylation
Y565 Phosphorylation
T567 Phosphorylation P17252 (PRKCA) , Q5S007 (LRRK2) , P41279 (MAP3K8) , O94804 (STK10) , O95819 (MAP4K4) , P25098 (GRK2) , P31751 (AKT2) , Q13464 (ROCK1) , Q04759 (PRKCQ) , Q9P289 (STK26) , P41743 (PRKCI) , O75116 (ROCK2)
T576 Phosphorylation

Research Backgrounds

Function:

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

PTMs:

Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).

S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Subcellular Location:

Apical cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection. Cell projection>Microvillus membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Cell projection>Microvillus.
Note: Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

Subunit Structure:

Interacts with MPP5 and SLC9A3R2. Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B. Interacts (when phosphorylated) with FES/FPS. Interacts with dimeric S100P, the interaction may be activating through unmasking of F-actin binding sites. Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Detected in a complex composed of at least EZR, AHNAK, PPL and PRX (By similarity). Interacts with PDPN (via cytoplasmic domain); activates RHOA and promotes epithelial-mesenchymal transition. Interacts with SPN, CD44 and ICAM2 (By similarity).

Family&Domains:

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Digestive system > Gastric acid secretion.

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