USP6 Antibody | Affinity Biosciences

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Western blot analysis USP6 using HeLa whole cell lysates.

DF9995 at 1/100 staining rat testis tissue by IHC-P.

Product:	USP6 Antibody
Catalog:	DF9995
Description:	Rabbit polyclonal antibody to USP6
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	159 kDa; 159kD(Calculated).
Uniprot:	P35125
RRID:	AB_2843187

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

USP6 Antibody detects endogenous levels of total USP6.

RRID:

AB_2843187
Cite Format: Affinity Biosciences Cat# DF9995, RRID:AB_2843187.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Deubiquitinating enzyme 6; HRP1; Proto-oncogene TRE-2; TRE17; TRE2; Ubiquitin carboxyl-terminal hydrolase 6; Ubiquitin specific protease 6; Ubiquitin thiolesterase 6; Ubiquitin-specific-processing protease 6; UBP6_HUMAN; USP6;

Immunogens

Immunogen:

Uniprot:

P35125(UBP6_HUMAN) >>Visit HPA database.

Gene(ID):

USP6(9098)

Expression:

P35125 UBP6_HUMAN:

Testis specific. Expressed in various cancer cell lines.

Sequence:

P35125 UBP6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDMVENADSLQAQERKDILMKYDKGHRAGLPEDKGPEPVGINSSIDRFGILHETELPPVTAREAKKIRREMTRTSKWMEMLGEWETYKHSSKLIDRVYKGIPMNIRGPVWSVLLNIQEIKLKNPGRYQIMKERGKRSSEHIHHIDLDVRTTLRNHVFFRDRYGAKQRELFYILLAYSEYNPEVGYCRDLSHITALFLLYLPEEDAFWALVQLLASERHSLPGFHSPNGGTVQGLQDQQEHVVPKSQPKTMWHQDKEGLCGQCASLGCLLRNLIDGISLGLTLRLWDVYLVEGEQVLMPITSIALKVQQKRLMKTSRCGLWARLRNQFFDTWAMNDDTVLKHLRASTKKLTRKQGDLPPPAKREQGSLAPRPVPASRGGKTLCKGYRQAPPGPPAQFQRPICSASPPWASRFSTPCPGGAVREDTYPVGTQGVPSLALAQGGPQGSWRFLEWKSMPRLPTDLDIGGPWFPHYDFEWSCWVRAISQEDQLATCWQAEHCGEVHNKDMSWPEEMSFTANSSKIDRQKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKSVAPLKLRRTIAKYAPKFDGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIIVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMDLEITVIKLDGTTPVRYGLRLNMDEKYTGLKKQLRDLCGLNSEQILLAEVHDSNIKNFPQDNQKVQLSVSGFLCAFEIPVPSSPISASSPTQIDFSSSPSTNGMFTLTTNGDLPKPIFIPNGMPNTVVPCGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSPQENRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSWLARPLPPQEASIHAQDRDNCMGYQYPFTLRVVQKDGNSCAWCPQYRFCRGCKIDCGEDRAFIGNAYIAVDWHPTALHLRYQTSQERVVDKHESVEQSRRAQAEPINLDSCLRAFTSEEELGESEMYYCSKCKTHCLATKKLDLWRLPPFLIIHLKRFQFVNDQWIKSQKIVRFLRESFDPSAFLVPRDPALCQHKPLTPQGDELSKPRILAREVKKVDAQSSAGKEDMLLSKSPSSLSANISSSPKGSPSSSRKSGTSCPSSKNSSPNSSPRTLGRSKGRLRLPQIGSKNKPSSSKKNLDASKENGAGQICELADALSRGHMRGGSQPELVTPQDHEVALANGFLYEHEACGNGCGDGYSNGQLGNHSEEDSTDDQREDTHIKPIYNLYAISCHSGILSGGHYITYAKNPNCKWYCYNDSSCEELHPDEIDTDSAYILFYEQQGIDYAQFLPKIDGKKMADTSSTDEDSESDYEKYSMLQ

PTMs - P35125 As Substrate

P35125

Site	PTM Type	Source
Y22	Phosphorylation	Uniprot
Y98	Phosphorylation	Uniprot
Y127	Phosphorylation	Uniprot
K361	Ubiquitination	Uniprot
S366	Phosphorylation	Uniprot
K578	Acetylation	Uniprot
K583	Acetylation	Uniprot
K645	Ubiquitination	Uniprot
K1141	Acetylation	Uniprot
K1142	Acetylation	Uniprot
S1147	Phosphorylation	Uniprot
K1158	Acetylation	Uniprot
S1159	Phosphorylation	Uniprot
S1168	Phosphorylation	Uniprot
S1169	Phosphorylation	Uniprot
S1188	Phosphorylation	Uniprot
S1191	Phosphorylation	Uniprot
S1192	Phosphorylation	Uniprot
S1196	Phosphorylation	Uniprot
T1199	Phosphorylation	Uniprot
S1214	Phosphorylation	Uniprot
T1391	Phosphorylation	Uniprot
S1395	Phosphorylation	Uniprot
Y1399	Phosphorylation	Uniprot

Research Backgrounds

P35125_UBP6_HUMAN

Function:

Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.

PTMs:

Monubiquitinated; ubiquitination is calmodulin and calcium dependent.

Subcellular Location:

Cell membrane. Cytoplasm. Endosome.
Note: Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.

Tissue Specificity:

Testis specific. Expressed in various cancer cell lines.

Subunit Structure:

Interacts with RAC1 and CDC42. Interacts (via Rab-GAP TBC domain) with ARF6. Interacts with calmodulin (CALM1, CALM2 and/or CALM3); the interaction is calcium-dependent.

Family&Domains:

The Rab-GAP TBC domain lacks GTPase activator activity but is necessary for interaction with ARF6.

Belongs to the peptidase C19 family.

Restrictive clause

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For Research Use Only.
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USP6 Antibody - #DF9995