USP49 Antibody | Affinity Biosciences

IHC
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DF9993 at 1/100 staining Human spleen tissue by IHC-P.

Product:	USP49 Antibody
Catalog:	DF9993
Description:	Rabbit polyclonal antibody to USP49
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	79 kDa; 79kD(Calculated).
Uniprot:	Q70CQ1
RRID:	AB_2843185

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

USP49 Antibody detects endogenous levels of total USP49.

RRID:

AB_2843185
Cite Format: Affinity Biosciences Cat# DF9993, RRID:AB_2843185.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Deubiquitinating enzyme 49; MGC20741; Ubiquitin carboxyl-terminal hydrolase 49; ubiquitin specific peptidase 49; Ubiquitin thiolesterase 49; Ubiquitin-specific-processing protease 49; UBP49; UBP49_HUMAN; USP49; USP49 ubiquitin specific peptidase 49;

Immunogens

Immunogen:

Uniprot:

Q70CQ1(UBP49_HUMAN) >>Visit HPA database.

Gene(ID):

USP49(25862)

Sequence:

Q70CQ1 UBP49_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDRCKHVGRLRLAQDHSILNPQKWCCLECATTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVRDLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDTPVRRGRTLRSMASGEDVVLPQRAPQGQPQMLTALWYRRQRLLARTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARRRRREVKRRLLEELASTPPRKSARLLLHTPRDAGPAASRPAALPTSRRVPAATLKLRRQPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKATNGKTQLSGKPTNSSATELSLRNDRAEACEREGFCWNGRASISRSLELIQNKEPSSKHISLCRELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTRRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFVPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGFWVHCNDSKLNVCSVEEVCKTQAYILFYTQRTVQGNARISETHLQAQVQSSNNDEGRPQTFS

PTMs - Q70CQ1 As Substrate

Q70CQ1

Site	PTM Type	Source
K93	Ubiquitination	Uniprot
T109	Phosphorylation	Uniprot
T116	Phosphorylation	Uniprot
T154	Phosphorylation	Uniprot
K306	Ubiquitination	Uniprot
R335	Methylation	Uniprot
T497	Phosphorylation	Uniprot
K522	Ubiquitination	Uniprot
K529	Ubiquitination	Uniprot

Research Backgrounds

Q70CQ1_UBP49_HUMAN

Function:

Specifically deubiquitinates histone H2B at 'Lys-120' (H2BK120Ub). H2BK120Ub is a specific tag for epigenetic transcriptional activation and acts as a regulator of mRNA splicing. Deubiquitination is required for efficient cotranscriptional splicing of a large set of exons.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of a complex with RUVBL1 and PSMC5.

Family&Domains:

Belongs to the peptidase C19 family.

References

1). Ubiquitin-specific protease 49 attenuates IL-1β-induced rat primary chondrocyte apoptosis by facilitating Axin deubiquitination and subsequent Wnt/β-catenin signaling cascade inhibition. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2020 (PubMed: 32737772) [IF=4.3]

Application: WB Species: rat Sample: chondrocytes

Fig. 1 | d Proliferation rates, e Usp49 mRNA levels, and f Usp49 protein levels of rat primary chondrocytes added with 5, 10, or 20 ng/mL IL-1β or PBS (the Vehicle group). *, **, and *** represent P values less than 0.05, 0.01,and 0.001, respectively, versus the Vehicle group; #P<0.05 versus the 5 ng/mL IL-1β group

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For Research Use Only.
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USP49 Antibody - #DF9993