USP34 Antibody - #DF9990

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Product: USP34 Antibody
Catalog: DF9990
Description: Rabbit polyclonal antibody to USP34
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 404 kDa; 404kD(Calculated).
Uniprot: Q70CQ2
RRID: AB_2843182

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(94%), Xenopus(81%)
Clonality:
Polyclonal
Specificity:
USP34 Antibody detects endogenous levels of total USP34.
RRID:
AB_2843182
Cite Format: Affinity Biosciences Cat# DF9990, RRID:AB_2843182.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Deubiquitinating enzyme 34; FLJ43910; KIAA0570; KIAA0729; MGC104459; Ubiquitin carboxyl terminal hydrolase 34; Ubiquitin carboxyl-terminal hydrolase 34; Ubiquitin specific processing protease 34; Ubiquitin specific protease 34; Ubiquitin thioesterase 34; Ubiquitin thiolesterase 34; Ubiquitin-specific-processing protease 34; UBP34_HUMAN; USP 34; Usp34;

Immunogens

Immunogen:
Uniprot:

Q70CQ2(UBP34_HUMAN) >>Visit HPA database.

Gene(ID):
USP34(9736)
Expression:
Q70CQ2 UBP34_HUMAN:

Expressed in brain at low level.

Sequence:
MCENCADLVEVLNEISDVEGGDGLQLRKEHTLKIFTYINSWTQRQCLCCFKEYKHLEIFNQVVCALINLVIAQVQVLRDQLCKHCTTINIDSTWQDESNQAEEPLNIDRECNEGSTERQKSIEKKSNSTRICNLTEEESSKSSDPFSLWSTDEKEKLLLCVAKIFQIQFPLYTAYKHNTHPTIEDISTQESNILGAFCDMNDVEVPLHLLRYVCLFCGKNGLSLMKDCFEYGTPETLPFLIAHAFITVVSNIRIWLHIPAVMQHIIPFRTYVIRYLCKLSDQELRQSAARNMADLMWSTVKEPLDTTLCFDKESLDLAFKYFMSPTLTMRLAGLSQITNQLHTFNDVCNNESLVSDTETSIAKELADWLISNNVVEHIFGPNLHIEIIKQCQVILNFLAAEGRLSTQHIDCIWAAAQLKHCSRYIHDLFPSLIKNLDPVPLRHLLNLVSALEPSVHTEQTLYLASMLIKALWNNALAAKAQLSKQSSFASLLNTNIPIGNKKEEEELRRTAPSPWSPAASPQSSDNSDTHQSGGSDIEMDEQLINRTKHVQQRLSDTEESMQGSSDETANSGEDGSSGPGSSSGHSDGSSNEVNSSHASQSAGSPGSEVQSEDIADIEALKEEDEDDDHGHNPPKSSCGTDLRNRKLESQAGICLGDSQGMSERNGTSSGTGKDLVFNTESLPSVDNRMRMLDACSHSEDPEHDISGEMNATHIAQGSQESCITRTGDFLGETIGNELFNCRQFIGPQHHHHHHHHHHHHDGHMVDDMLSADDVSCSSSQVSAKSEKNMADFDGEESGCEEELVQINSHAELTSHLQQHLPNLASIYHEHLSQGPVVHKHQFNSNAVTDINLDNVCKKGNTLLWDIVQDEDAVNLSEGLINEAEKLLCSLVCWFTDRQIRMRFIEGCLENLGNNRSVVISLRLLPKLFGTFQQFGSSYDTHWITMWAEKELNMMKLFFDNLVYYIQTVREGRQKHALYSHSAEVQVRLQFLTCVFSTLGSPDHFRLSLEQVDILWHCLVEDSECYDDALHWFLNQVRSKDQHAMGMETYKHLFLEKMPQLKPETISMTGLNLFQHLCNLARLATSAYDGCSNSELCGMDQFWGIALRAQSGDVSRAAIQYINSYYINGKTGLEKEQEFISKCMESLMIASSSLEQESHSSLMVIERGLLMLKTHLEAFRRRFAYHLRQWQIEGTGISSHLKALSDKQSLPLRVVCQPAGLPDKMTIEMYPSDQVADLRAEVTHWYENLQKEQINQQAQLQEFGQSNRKGEFPGGLMGPVRMISSGHELTTDYDEKALHELGFKDMQMVFVSLGAPRRERKGEGVQLPASCLPPPQKDNIPMLLLLQEPHLTTLFDLLEMLASFKPPSGKVAVDDSESLRCEELHLHAENLSRRVWELLMLLPTCPNMLMAFQNISDEQSNDGFNWKELLKIKSAHKLLYALEIIEALGKPNRRIRRESTGSYSDLYPDSDDSSEDQVENSKNSWSCKFVAAGGLQQLLEIFNSGILEPKEQESWTVWQLDCLACLLKLICQFAVDPSDLDLAYHDVFAWSGIAESHRKRTWPGKSRKAAGDHAKGLHIPRLTEVFLVLVQGTSLIQRLMSVAYTYDNLAPRVLKAQSDHRSRHEVSHYSMWLLVSWAHCCSLVKSSLADSDHLQDWLKKLTLLIPETAVRHESCSGLYKLSLSGLDGGDSINRSFLLLAASTLLKFLPDAQALKPIRIDDYEEEPILKPGCKEYFWLLCKLVDNIHIKDASQTTLLDLDALARHLADCIRSREILDHQDGNVEDDGLTGLLRLATSVVKHKPPFKFSREGQEFLRDIFNLLFLLPSLKDRQQPKCKSHSSRAAAYDLLVEMVKGSVENYRLIHNWVMAQHMQSHAPYKWDYWPHEDVRAECRFVGLTNLGATCYLASTIQQLYMIPEARQAVFTAKYSEDMKHKTTLLELQKMFTYLMESECKAYNPRPFCKTYTMDKQPLNTGEQKDMTEFFTDLITKIEEMSPELKNTVKSLFGGVITNNVVSLDCEHVSQTAEEFYTVRCQVADMKNIYESLDEVTIKDTLEGDNMYTCSHCGKKVRAEKRACFKKLPRILSFNTMRYTFNMVTMMKEKVNTHFSFPLRLDMTPYTEDFLMGKSERKEGFKEVSDHSKDSESYEYDLIGVTVHTGTADGGHYYSFIRDIVNPHAYKNNKWYLFNDAEVKPFDSAQLASECFGGEMTTKTYDSVTDKFMDFSFEKTHSAYMLFYKRMEPEEENGREYKFDVSSELLEWIWHDNMQFLQDKNIFEHTYFGFMWQLCSCIPSTLPDPKAVSLMTAKLSTSFVLETFIHSKEKPTMLQWIELLTKQFNNSQAACEWFLDRMADDDWWPMQILIKCPNQIVRQMFQRLCIHVIQRLRPVHAHLYLQPGMEDGSDDMDTSVEDIGGRSCVTRFVRTLLLIMEHGVKPHSKHLTEYFAFLYEFAKMGEEESQFLLSLQAISTMVHFYMGTKGPENPQVEVLSEEEGEEEEEEEDILSLAEEKYRPAALEKMIALVALLVEQSRSERHLTLSQTDMAALTGGKGFPFLFQHIRDGINIRQTCNLIFSLCRYNNRLAEHIVSMLFTSIAKLTPEAANPFFKLLTMLMEFAGGPPGMPPFASYILQRIWEVIEYNPSQCLDWLAVQTPRNKLAHSWVLQNMENWVERFLLAHNYPRVRTSAAYLLVSLIPSNSFRQMFRSTRSLHIPTRDLPLSPDTTVVLHQVYNVLLGLLSRAKLYVDAAVHGTTKLVPYFSFMTYCLISKTEKLMFSTYFMDLWNLFQPKLSEPAIATNHNKQALLSFWYNVCADCPENIRLIVQNPVVTKNIAFNYILADHDDQDVVLFNRGMLPAYYGILRLCCEQSPAFTRQLASHQNIQWAFKNLTPHASQYPGAVEELFNLMQLFIAQRPDMREEELEDIKQFKKTTISCYLRCLDGRSCWTTLISAFRILLESDEDRLLVVFNRGLILMTESFNTLHMMYHEATACHVTGDLVELLSIFLSVLKSTRPYLQRKDVKQALIQWQERIEFAHKLLTLLNSYSPPELRNACIDVLKELVLLSPHDFLHTLVPFLQHNHCTYHHSNIPMSLGPYFPCRENIKLIGGKSNIRPPRPELNMCLLPTMVETSKGKDDVYDRMLLDYFFSYHQFIHLLCRVAINCEKFTETLVKLSVLVAYEGLPLHLALFPKLWTELCQTQSAMSKNCIKLLCEDPVFAEYIKCILMDERTFLNNNIVYTFMTHFLLKVQSQVFSEANCANLISTLITNLISQYQNLQSDFSNRVEISKASASLNGDLRALALLLSVHTPKQLNPALIPTLQELLSKCRTCLQQRNSLQEQEAKERKTKDDEGATPIKRRRVSSDEEHTVDSCISDMKTETREVLTPTSTSDNETRDSSIIDPGTEQDLPSPENSSVKEYRMEVPSSFSEDMSNIRSQHAEEQSNNGRYDDCKEFKDLHCSKDSTLAEEESEFPSTSISAVLSDLADLRSCDGQALPSQDPEVALSLSCGHSRGLFSHMQQHDILDTLCRTIESTIHVVTRISGKGNQAAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
94
Xenopus
81
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q70CQ2 As Substrate

Site PTM Type Enzyme
S98 Phosphorylation
K141 Ubiquitination
K154 Ubiquitination
S187 Phosphorylation
T188 Phosphorylation
S298 Phosphorylation
K301 Ubiquitination
K312 Acetylation
Y321 Phosphorylation
S324 Phosphorylation
T326 Phosphorylation
T328 Phosphorylation
S352 Phosphorylation
S355 Phosphorylation
S465 Phosphorylation
S483 Phosphorylation
K484 Ubiquitination
S486 Phosphorylation
S487 Phosphorylation
S490 Phosphorylation
S590 Phosphorylation
S649 Phosphorylation
S658 Phosphorylation
T679 Phosphorylation
S681 Phosphorylation
S718 Phosphorylation Q13315 (ATM)
Y827 Phosphorylation
S844 Phosphorylation
K857 Ubiquitination
S920 Phosphorylation
K1039 Ubiquitination
K1134 Ubiquitination
T1194 Phosphorylation
S1197 Phosphorylation
S1198 Phosphorylation
S1204 Phosphorylation
K1206 Ubiquitination
S1208 Phosphorylation
K1250 Ubiquitination
S1283 Phosphorylation
S1284 Phosphorylation
S1458 Phosphorylation
T1459 Phosphorylation
S1461 Phosphorylation
Y1462 Phosphorylation
S1469 Phosphorylation
S1472 Phosphorylation
S1473 Phosphorylation
S1480 Phosphorylation
S1503 Phosphorylation
K1564 Ubiquitination
K1567 Ubiquitination
K1574 Ubiquitination
S1650 Phosphorylation
K1658 Ubiquitination
K1714 Ubiquitination
K1728 Ubiquitination
K1748 Ubiquitination
S1751 Phosphorylation Q13315 (ATM)
S1826 Phosphorylation
S1950 Phosphorylation
K1968 Ubiquitination
K1977 Ubiquitination
T2010 Phosphorylation
S2015 Phosphorylation
Y2042 Phosphorylation
Y2060 Phosphorylation
S2085 Phosphorylation
Y2091 Phosphorylation
T2092 Phosphorylation
T2097 Phosphorylation
K2100 Ubiquitination
K2102 Ubiquitination
T2116 Phosphorylation
K2126 Ubiquitination
K2219 Ubiquitination
S2255 Phosphorylation
K2334 Ubiquitination
K2363 Ubiquitination
S2488 Phosphorylation
K2508 Ubiquitination
Y2677 Phosphorylation
S2690 Phosphorylation
S2694 Phosphorylation
S2703 Phosphorylation
T2704 Phosphorylation
T2711 Phosphorylation
T2721 Phosphorylation
S2865 Phosphorylation
S3007 Phosphorylation
Y3011 Phosphorylation
K3100 Ubiquitination
K3105 Ubiquitination
K3205 Ubiquitination
K3322 Ubiquitination
K3339 Acetylation
T3350 Phosphorylation
S3358 Phosphorylation
S3359 Phosphorylation
T3364 Phosphorylation
T3376 Phosphorylation
T3381 Phosphorylation
S3384 Phosphorylation
T3385 Phosphorylation
S3386 Phosphorylation
S3393 Phosphorylation
S3394 Phosphorylation
T3400 Phosphorylation
S3406 Phosphorylation
S3410 Phosphorylation
S3411 Phosphorylation
S3501 Phosphorylation
S3503 Phosphorylation
S3507 Phosphorylation

Research Backgrounds

Function:

Ubiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.

Tissue Specificity:

Expressed in brain at low level.

Subunit Structure:

Interacts with AXIN1 and AXIN2.

Family&Domains:

Belongs to the peptidase C19 family.

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