USP3 Antibody - #DF9988
| Product: | USP3 Antibody |
| Catalog: | DF9988 |
| Description: | Rabbit polyclonal antibody to USP3 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 39 kDa; 59kD(Calculated). |
| Uniprot: | Q9Y6I4 |
| RRID: | AB_2843180 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9988, RRID:AB_2843180.
Fold/Unfold
Deubiquitinating enzyme 3; EC 3.1.2.15; SIH003; Ubiquitin carboxyl-terminal hydrolase 3; Ubiquitin specific peptidase 3; Ubiquitin specific processing protease 3; Ubiquitin Specific Protease 3; Ubiquitin thioesterase 3; Ubiquitin thiolesterase 3; Ubiquitin-specific-processing protease 3; UBP; UBP3_HUMAN; Usp3;
Immunogens
- Q9Y6I4 UBP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MECPHLSSSVCIAPDSAKFPNGSPSSWCCSVCRSNKSPWVCLTCSSVHCGRYVNGHAKKHYEDAQVPLTNHKKSEKQDKVQHTVCMDCSSYSTYCYRCDDFVVNDTKLGLVQKVREHLQNLENSAFTADRHKKRKLLENSTLNSKLLKVNGSTTAICATGLRNLGNTCFMNAILQSLSNIEQFCCYFKELPAVELRNGKTAGRRTYHTRSQGDNNVSLVEEFRKTLCALWQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLELQGGFNGVSRSAILQENSTLSASNKCCINGASTVVTAIFGGILQNEVNCLICGTESRKFDPFLDLSLDIPSQFRSKRSKNQENGPVCSLRDCLRSFTDLEELDETELYMCHKCKKKQKSTKKFWIQKLPKVLCLHLKRFHWTAYLRNKVDTYVEFPLRGLDMKCYLLEPENSGPESCLYDLAAVVVHHGSGVGSGHYTAYATHEGRWFHFNDSTVTLTDEETVVKAKAYILFYVEHQAKAGSDKL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6I4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| K107 | Sumoylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K354 | Methylation | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| T372 | Phosphorylation | Uniprot | |
| K423 | Ubiquitination | Uniprot |
Research Backgrounds
Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
Nucleus.
Note: Localizes preferentially with monoubiquitinated H2A to chromatin.
Expressed in all tissues examined, with strongest expression in pancreas.
Interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A.
Both protease activity and an intact zinc finger are required for H2A monodeubiquitination.
Belongs to the peptidase C19 family. USP3 subfamily.
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