USP16 Antibody - #DF9983
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9983, RRID:AB_2843175.
Fold/Unfold
Deubiquitinating enzyme 16; Human ubiquitin processing protease; MSTP039; Ubiquitin carboxyl-terminal hydrolase 16; Ubiquitin Specific peptidase 16; Ubiquitin Specific Protease 16; Ubiquitin thiolesterase 16; Ubiquitin-processing protease UBP-M; Ubiquitin-specific-processing protease 16; UBP M; UBP16_HUMAN; UBPM; usp16;
Immunogens
Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Q9Y5T5 UBP16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKKRTKGKTVPIDDSSETLEPVCRHIRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKAEEETEEKPSVWLCLKCGHQGCGRNSQEQHALKHYLTPRSEPHCLVLSLDNWSVWCYVCDNEVQYCSSNQLGQVVDYVRKQASITTPKPAEKDNGNIELENKKLEKESKNEQEREKKENMAKENPPMNSPCQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQETKKGVVTPKELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKKKSMPSFVDRIFGGELTSMIMCDQCRTVSLVHESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKDNDSYIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDICTIDHPEDSEYEAEMSLQGEVNIKSNHISQEGVMHKEYCVNQKDLNGQAKMIESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTEGSNGEVDISNGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTLANREVFNTDECSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIKGERKHVYTNAKKQMLISLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANSHLSNLVLHGDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYERIL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y5T5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| K29 | Acetylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| K37 | Acetylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| S330 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K333 | Ubiquitination | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S415 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K437 | Ubiquitination | Uniprot | |
| S486 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K506 | Ubiquitination | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| S531 | Phosphorylation | Uniprot | |
| T550 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T554 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| T600 | Phosphorylation | Uniprot | |
| Y606 | Phosphorylation | Uniprot | |
| Y742 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| K764 | Ubiquitination | Uniprot |
Research Backgrounds
Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.
Nucleus.
Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Homotetramer.
The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.
Belongs to the peptidase C19 family. USP16 subfamily.
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