USP14 Antibody | Affinity Biosciences

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Western blot analysis of extracts from B16F10 cells, using USP14 Antibody.

Product:	USP14 Antibody
Catalog:	DF9982
Description:	Rabbit polyclonal antibody to USP14
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	56 kDa; 56kD(Calculated).
Uniprot:	P54578
RRID:	AB_2843174

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)

Clonality:

Polyclonal

Specificity:

USP14 Antibody detects endogenous levels of total USP14.

RRID:

AB_2843174
Cite Format: Affinity Biosciences Cat# DF9982, RRID:AB_2843174.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Deubiquitinating enzyme 14; TGT; tRNA guanine transglycosylase 60 kD subunit; tRNA guanine transglycosylase; Ubiquitin carboxyl terminal hydrolase 14; Ubiquitin carboxyl-terminal hydrolase 14; Ubiquitin specific peptidase 14; Ubiquitin specific processing protease 14; Ubiquitin specific protease 14; Ubiquitin thiolesterase 14; Ubiquitin-specific-processing protease 14; UBP14_HUMAN; USP 14; USP14;

Immunogens

Immunogen:

Uniprot:

P54578(UBP14_HUMAN) >>Visit HPA database.

Gene(ID):

USP14(9097)

Sequence:

P54578 UBP14_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54578 As Substrate

P54578

Site	PTM Type	Enzyme	Source
Y4	Phosphorylation		Uniprot
K14	Acetylation		Uniprot
K49	Acetylation		Uniprot
K49	Ubiquitination		Uniprot
T52	Phosphorylation		Uniprot
K54	Ubiquitination		Uniprot
K62	Ubiquitination		Uniprot
S74	Phosphorylation		Uniprot
S83	Phosphorylation		Uniprot
K130	Ubiquitination		Uniprot
K134	Ubiquitination		Uniprot
Y136	Phosphorylation		Uniprot
S143	Phosphorylation		Uniprot
S148	Phosphorylation		Uniprot
Y151	Phosphorylation		Uniprot
K214	Ubiquitination		Uniprot
S222	Phosphorylation		Uniprot
K224	Ubiquitination		Uniprot
S228	Phosphorylation		Uniprot
S229	Phosphorylation		Uniprot
S230	Phosphorylation		Uniprot
S232	Phosphorylation		Uniprot
T235	Phosphorylation	P06493 (CDK1)	Uniprot
S237	Phosphorylation		Uniprot
K238	Ubiquitination		Uniprot
K239	Ubiquitination		Uniprot
S241	Phosphorylation		Uniprot
K269	Ubiquitination		Uniprot
S276	Phosphorylation		Uniprot
K284	Acetylation		Uniprot
K284	Ubiquitination		Uniprot
Y285	Phosphorylation		Uniprot
K291	Acetylation		Uniprot
K291	Ubiquitination		Uniprot
R293	Methylation		Uniprot
T299	Phosphorylation		Uniprot
K300	Ubiquitination		Uniprot
S302	Phosphorylation		Uniprot
K313	Acetylation		Uniprot
K313	Ubiquitination		Uniprot
S314	Phosphorylation		Uniprot
S315	Phosphorylation		Uniprot
K334	Ubiquitination		Uniprot
K342	Ubiquitination		Uniprot
K348	Acetylation		Uniprot
K348	Ubiquitination		Uniprot
K373	Ubiquitination		Uniprot
K375	Ubiquitination		Uniprot
K381	Ubiquitination		Uniprot
K391	Acetylation		Uniprot
K391	Ubiquitination		Uniprot
S393	Phosphorylation		Uniprot
S394	Phosphorylation		Uniprot
K400	Ubiquitination		Uniprot
S412	Phosphorylation		Uniprot
Y417	Phosphorylation		Uniprot
S432	Phosphorylation	P31749 (AKT1)	Uniprot
Y436	Phosphorylation		Uniprot
K441	Ubiquitination		Uniprot
K443	Ubiquitination		Uniprot
K449	Acetylation		Uniprot
K449	Ubiquitination		Uniprot
K454	Acetylation		Uniprot
K454	Ubiquitination		Uniprot
T459	Phosphorylation		Uniprot
S492	Phosphorylation		Uniprot

Research Backgrounds

P54578_UBP14_HUMAN

Function:

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein.

Subunit Structure:

Homodimer (Potential). Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1. Interacts with TRIM14; this interaction recruits USP14 to cleave ubiquitin chains of CGAS.

Family&Domains:

Belongs to the peptidase C19 family. USP14/UBP6 subfamily.

References

1). Therapeutic potential of alkaloid extract from Codonopsis Radix in alleviating hepatic lipid accumulation: insights into mitochondrial energy metabolism and endoplasmic reticulum stress regulation in NAFLD mice. Chinese Journal of Natural Medicines, 2023 (PubMed: 37407172) [IF=4.6]

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USP14 Antibody - #DF9982