TJP3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of TJP3 using RAW264.

DF9961 at 1/100 staining rat brain tissue by IHC-P.

Product:	TJP3 Antibody
Catalog:	DF9961
Description:	Rabbit polyclonal antibody to TJP3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Dog, Chicken
Mol.Wt.:	101 kDa; 101kD(Calculated).
Uniprot:	O95049
RRID:	AB_2843155

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Dog(83%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

TJP3 Antibody detects endogenous levels of total TJP3.

RRID:

AB_2843155
Cite Format: Affinity Biosciences Cat# DF9961, RRID:AB_2843155.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Tight junction protein 3; Tight junction protein ZO-3; Tight junction protein ZO3; TJP3; ZO3; ZO3_HUMAN; Zona occludens protein 3; Zonula occludens protein 3;

Immunogens

Immunogen:

Uniprot:

O95049(ZO3_HUMAN) >>Visit HPA database.

Gene(ID):

TJP3(27134)

Sequence:

O95049 ZO3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEELTIWEQHTATLSKDPRRGFGIAISGGRDRPGGSMVVSDVVPGGPAEGRLQTGDHIVMVNGVSMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRVEEVDQGRGYDGDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMKPVKSVLVKRRDSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSEGKLSLLVLRDRGQFLVNIPPAVSDSDSSPLEDISDLASELSQAPPSHIPPPPRHAQRSPEASQTDSPVESPRLRRESSVDSRTISEPDEQRSELPRESSYDIYRVPSSQSMEDRGYSPDTRVVRFLKGKSIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDQILQVNDVPFQNLTREEAVQFLLGLPPGEEMELVTQRKQDIFWKMVQSRVGDSFYIRTHFELEPSPPSGLGFTRGDVFHVLDTLHPGPGQSHARGGHWLAVRMGRDLREQERGIIPNQSRAEQLASLEAAQRAVGVGPGSSAGSNARAEFWRLRGLRRGAKKTTQRSREDLSALTRQGRYPPYERVVLREASFKRPVVILGPVADIAMQKLTAEMPDQFEIAETVSRTDSPSKIIKLDTVRVIAEKDKHALLDVTPSAIERLNYVQYYPIVVFFIPESRPALKALRQWLAPASRRSTRRLYAQAQKLRKHSSHLFTATIPLNGTSDTWYQELKAIIREQQTRPIWTAEDQLDGSLEDNLDLPHHGLADSSADLSCDSRVNSDYETDGEGGAYTDGEGYTDGEGGPYTDVDDEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Dog

Chicken

Bovine

Sheep

Xenopus

Horse

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95049 As Substrate

O95049

Site	PTM Type	Source
S27	Phosphorylation	Uniprot
S36	Phosphorylation	Uniprot
S103	Phosphorylation	Uniprot
S106	Phosphorylation	Uniprot
S112	Phosphorylation	Uniprot
Y130	Phosphorylation	Uniprot
S134	Phosphorylation	Uniprot
S135	Phosphorylation	Uniprot
S136	Phosphorylation	Uniprot
S138	Phosphorylation	Uniprot
S159	Phosphorylation	Uniprot
S164	Phosphorylation	Uniprot
S169	Phosphorylation	Uniprot
S203	Phosphorylation	Uniprot
S212	Phosphorylation	Uniprot
S222	Phosphorylation	Uniprot
K259	Ubiquitination	Uniprot
S265	Phosphorylation	Uniprot
S319	Phosphorylation	Uniprot
S323	Phosphorylation	Uniprot
T325	Phosphorylation	Uniprot
S327	Phosphorylation	Uniprot
S331	Phosphorylation	Uniprot
S338	Phosphorylation	Uniprot
S339	Phosphorylation	Uniprot
S342	Phosphorylation	Uniprot
T344	Phosphorylation	Uniprot
S346	Phosphorylation	Uniprot
R352	Methylation	Uniprot
R357	Methylation	Uniprot
S360	Phosphorylation	Uniprot
Y361	Phosphorylation	Uniprot
Y364	Phosphorylation	Uniprot
S368	Phosphorylation	Uniprot
S369	Phosphorylation	Uniprot
S371	Phosphorylation	Uniprot
S378	Phosphorylation	Uniprot
Y479	Phosphorylation	Uniprot
S489	Phosphorylation	Uniprot
R518	Methylation	Uniprot
S564	Phosphorylation	Uniprot
S591	Phosphorylation	Uniprot
T599	Phosphorylation	Uniprot
S616	Phosphorylation	Uniprot
S654	Phosphorylation	Uniprot
T679	Phosphorylation	Uniprot
S681	Phosphorylation	Uniprot
S778	Phosphorylation	Uniprot
S805	Phosphorylation	Uniprot
S856	Phosphorylation	Uniprot
S864	Phosphorylation	Uniprot
S884	Phosphorylation	Uniprot
S905	Phosphorylation	Uniprot
S906	Phosphorylation	Uniprot

Research Backgrounds

O95049_ZO3_HUMAN

Function:

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions. Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition. With TJP1 and TJP2, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (By similarity).

PTMs:

Phosphorylated (By similarity).

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Nucleus.
Note: Exhibits predominant nuclear expression in proliferating cells but is exclusively junctionally expressed after confluence is reached (PubMed:23608536). Shows an epithelial-specific tight junction localization in a TJP1/TJP2-dependent fashion (By similarity).

Subunit Structure:

Interacts with occludin OCLN, claudins and TPJ1 (By similarity). Interacts with PATJ (By similarity). Interacts with UBN1. Interacts with FASLG. Interacts with CCND1.

Family&Domains:

Belongs to the MAGUK family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

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For Research Use Only.
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TJP3 Antibody - #DF9961