ZNF280B Antibody | Affinity Biosciences

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Western blot analysis of extracts from rat brain, using ZNF280B Antibody.

DF9950 at 1/100 staining Rat brain tissue by IHC-P.

Product:	ZNF280B Antibody
Catalog:	DF9950
Description:	Rabbit polyclonal antibody to ZNF280B
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	62 kDa; 62kD(Calculated).
Uniprot:	Q86YH2
RRID:	AB_2843144

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(90%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ZNF280B Antibody detects endogenous levels of total ZNF280B.

RRID:

AB_2843144
Cite Format: Affinity Biosciences Cat# DF9950, RRID:AB_2843144.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5''OY11.1; D87009.C22.3; SUHW2; Suppressor of hairy wing homolog 2; Z280B_HUMAN; Zinc finger protein 279; Zinc finger protein 280B; Zinc finger protein 632; ZNF279; ZNF280B; ZNF632;

Immunogens

Immunogen:

Uniprot:

Q86YH2(Z280B_HUMAN) >>Visit HPA database.

Gene(ID):

ZNF280B(140883)

Sequence:

Q86YH2 Z280B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEQSCEEEKEPEPQKNIQETKQVDDEDAELIFVGVEHVNEDAELIFVGVTSNSKPVVSNILNRVTPGSWSRRKKYDHLRKDTARKLQPKSHETVTSEAVTVLPASQLESRSTDSPIIIEPLSKPDYRNSSPQVVPNNSSELPSPLITFTDSLHHPVSTALSVGGINESPRVSKQLSTFEVNSINPKRAKLRDGIIEGNSSASFPSDTFHTMNTQQSTPSNNVHTSLSHVQNGAPFPAAFPKDNIHFKPINTNLDRENELAKTDILSLTSQNKTFDPKKENPIVLLSDFYYGQHKGEGQPEQKTHTTFKCLSCVKVLKNVKFMNHVKHHLEFEKQRNDSWENHTTCQHCHRQFPTPFQLQCHIENVHTAQEPSTVCKICELSFETDQVLLQHMKDHHKPGEMPYVCQVCHYRSSVFADVETHFRTCHENTKNLLCPFCLKIFKTATPYMCHYRGHWGKSAHQCSKCRLQFLTFKEKMEHKTQCHQMFKKPKQLEGLPPETKVTIQVSLEPLQPGSVDVASITVSTSDSEPSLPRSKSKISKKSH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Rabbit

100

Bovine

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86YH2 As Substrate

Q86YH2

Site	PTM Type	Source
M1	Acetylation	Uniprot
S68	Phosphorylation	Uniprot
S70	Phosphorylation	Uniprot
S105	Phosphorylation	Uniprot
S114	Phosphorylation	Uniprot
T262	Phosphorylation	Uniprot
S266	Phosphorylation	Uniprot
T268	Phosphorylation	Uniprot
Y451	Phosphorylation	Uniprot

Research Backgrounds

Q86YH2_Z280B_HUMAN

Function:

May function as a transcription factor.

Subcellular Location:

Nucleus.

References

1). microRNA‑196a‑3p inhibits cell proliferation and promotes cell apoptosis by targeting ADP ribosylation factor 4 in diffuse large B‑cell lymphoma. ONCOLOGY REPORTS, 2021 (PubMed: 33416178) [IF=4.2]

Application: WB Species: Human Sample: DLBCL cells

Figure 4. - Prediction and identification of miR-196a-3p target genes in DLBCL cells. (A) miRNA targets were predicted using TargetScan, microRNA, miRDB and DIANA-microT. (B) The protein levels of 4 miR-196a-3p candidate target genes were analyzed by western blot analysis. Up, protein bands; down, grey value. (C) mRNA levels of 4 miR-196a-3p candidate target genes were analyzed by RT-qPCR. (D) 293T cells were transiently transfected with Renilla luciferase reporter vectors containing either wild-type or mutant ARF4 3′-UTR seed sequence, and luciferase activity was measured following co-transfection with miR-196a-3p or NC. *P

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ZNF280B Antibody - #DF9950