SLC28A1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/4

Western blot analysis of extracts from Rat heart, using SLC28A1 Antibody.

DF9939 at 1/100 staining mouse kidney tissue by IHC-P.

DF9939 staining A549 cells by IF/ICC.

Figure 2 |Effects of the expression changes in the expression of nucleoside-metabolizing enzyme induced by ARS in the hippocampus of mice.

Product:	SLC28A1 Antibody
Catalog:	DF9939
Description:	Rabbit polyclonal antibody to SLC28A1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	72 kDa; 72kD(Calculated).
Uniprot:	O00337
RRID:	AB_2843133

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

SLC28A1 Antibody detects endogenous levels of total SLC28A1.

RRID:

AB_2843133
Cite Format: Affinity Biosciences Cat# DF9939, RRID:AB_2843133.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CNT 1; CNT1; Concentrative nucleoside transporter 1; hCNT1; Na(+)/nucleoside cotransporter 1; S28A1_HUMAN; SLC28A1; Sodium-coupled nucleoside transporter 1; Sodium/nucleoside cotransporter 1; solute carrier family 28 concentrative nucleoside transporter member 1; Solute carrier family 28 member 1;

Immunogens

Immunogen:

Uniprot:

O00337(S28A1_HUMAN) >>Visit HPA database.

Gene(ID):

SLC28A1(9154)

Expression:

O00337 S28A1_HUMAN:

Expressed in kidney.

Sequence:

O00337 S28A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENDPSRRRESISLTPVAKGLENMGADFLESLEEGQLPRSDLSPAEIRSSWSEAAPKPFSRWRNLQPALRARSFCREHMQLFRWIGTGLLCTGLSAFLLVACLLDFQRALALFVLTCVVLTFLGHRLLKRLLGPKLRRFLKPQGHPRLLLWFKRGLALAAFLGLVLWLSLDTSQRPEQLVSFAGICVFVALLFACSKHHCAVSWRAVSWGLGLQFVLGLLVIRTEPGFIAFEWLGEQIRIFLSYTKAGSSFVFGEALVKDVFAFQVLPIIVFFSCVISVLYHVGLMQWVILKIAWLMQVTMGTTATETLSVAGNIFVSQTEAPLLIRPYLADMTLSEVHVVMTGGYATIAGSLLGAYISFGIDATSLIAASVMAAPCALALSKLVYPEVEESKFRREEGVKLTYGDAQNLIEAASTGAAISVKVVANIAANLIAFLAVLDFINAALSWLGDMVDIQGLSFQLICSYILRPVAFLMGVAWEDCPVVAELLGIKLFLNEFVAYQDLSKYKQRRLAGAEEWVGDRKQWISVRAEVLTTFALCGFANFSSIGIMLGGLTSMVPQRKSDFSQIVLRALFTGACVSLVNACMAGILYMPRGAEVDCMSLLNTTLSSSSFEIYQCCREAFQSVNPEFSPEALDNCCRFYNHTICAQ

PTMs - O00337 As Substrate

O00337

Site	PTM Type	Source
S11	Phosphorylation	Uniprot
S13	Phosphorylation	Uniprot
S43	Phosphorylation	Uniprot
T403	Phosphorylation	Uniprot
Y404	Phosphorylation	Uniprot

Research Backgrounds

O00337_S28A1_HUMAN

Function:

Sodium-dependent and pyrimidine-selective transporter. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). Transports uridine, cytidine, thymidine, and nucleoside-derived drugs. Transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). It may be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS). It has the following selective inhibition: adenosine, thymidine, cytidine, uridine >> guanosine, inosine.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed in kidney.

Family&Domains:

Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.

References

1). e5NT inhibitor protects acute restraint stress‐induced depression by regulating nucleoside release in mice. JOURNAL OF PHARMACY AND PHARMACOLOGY, 2020 (PubMed: 32743802) [IF=3.3]

Application: WB Species: mouse Sample: hippocampus

Figure 2 |Effects of the expression changes in the expression of nucleoside-metabolizing enzyme induced by ARS in the hippocampus of mice.There were no significant changes in the expression of CNT1, CNT2 (a), ENT1 and ENT2 (b).

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SLC28A1 Antibody - #DF9939