SLC4A7 Antibody - #DF9927

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Product: SLC4A7 Antibody
Catalog: DF9927
Description: Rabbit polyclonal antibody to SLC4A7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 136kDa; 136kD(Calculated).
Uniprot: Q9Y6M7
RRID: AB_2843121

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
SLC4A7 Antibody detects endogenous levels of total SLC4A7.
RRID:
AB_2843121
Cite Format: Affinity Biosciences Cat# DF9927, RRID:AB_2843121.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Bicarbonate transporter; BT; NBC2; NBC3; S4A7; SBC2; SLC4A6; Sodium bicarbonate cotransporter 2; Sodium bicarbonate cotransporter 2b; Sodium bicarbonate cotransporter 3; Solute carrier family 4 member 7; Solute carrier family 4 sodium bicarbonate cotransporter member 7;

Immunogens

Immunogen:
Uniprot:

Q9Y6M7(S4A7_HUMAN) >>Visit HPA database.

Gene(ID):
SLC4A7(9497)
Expression:
Q9Y6M7 S4A7_HUMAN:

Highly expressed in testis and spleen. Also expressed in retina, colon, small intestine, ovary, thymus, prostate, muscle, heart and kidney. Isoform 1 is expressed in skeletal muscle and heart muscle.

Sequence:
MERFRLEKKLPGPDEEAVVDLGKTSSTVNTKFEKEELESHRAVYIGVHVPFSKESRRRHRHRGHKHHHRRRKDKESDKEDGRESPSYDTPSQRVQFILGTEDDDEEHIPHDLFTEMDELCYRDGEEYEWKETARWLKFEEDVEDGGDRWSKPYVATLSLHSLFELRSCILNGTVMLDMRASTLDEIADMVLDNMIASGQLDESIRENVREALLKRHHHQNEKRFTSRIPLVRSFADIGKKHSDPHLLERNGEGLSASRHSLRTGLSASNLSLRGESPLSLLLGHLLPSSRAGTPAGSRCTTPVPTPQNSPPSSPSISRLTSRSSQESQRQAPELLVSPASDDIPTVVIHPPEEDLEAALKGEEQKNEENVDLTPGILASPQSAPGNLDNSKSGEIKGNGSGGSRENSTVDFSKVDMNFMRKIPTGAEASNVLVGEVDFLERPIIAFVRLAPAVLLTGLTEVPVPTRFLFLLLGPAGKAPQYHEIGRSIATLMTDEIFHDVAYKAKDRNDLLSGIDEFLDQVTVLPPGEWDPSIRIEPPKSVPSQEKRKIPVFHNGSTPTLGETPKEAAHHAGPELQRTGRLFGGLILDIKRKAPFFLSDFKDALSLQCLASILFLYCACMSPVITFGGLLGEATEGRISAIESLFGASLTGIAYSLFAGQPLTILGSTGPVLVFEKILYKFCRDYQLSYLSLRTSIGLWTSFLCIVLVATDASSLVCYITRFTEEAFAALICIIFIYEALEKLFDLGETYAFNMHNNLDKLTSYSCVCTEPPNPSNETLAQWKKDNITAHNISWRNLTVSECKKLRGVFLGSACGHHGPYIPDVLFWCVILFFTTFFLSSFLKQFKTKRYFPTKVRSTISDFAVFLTIVIMVTIDYLVGVPSPKLHVPEKFEPTHPERGWIISPLGDNPWWTLLIAAIPALLCTILIFMDQQITAVIINRKEHKLKKGAGYHLDLLMVGVMLGVCSVMGLPWFVAATVLSISHVNSLKVESECSAPGEQPKFLGIREQRVTGLMIFILMGLSVFMTSVLKFIPMPVLYGVFLYMGVSSLKGIQLFDRIKLFGMPAKHQPDLIYLRYVPLWKVHIFTVIQLTCLVLLWVIKVSAAAVVFPMMVLALVFVRKLMDLCFTKRELSWLDDLMPESKKKKEDDKKKKEKEEAERMLQDDDDTVHLPFEGGSLLQIPVKALKYSPDKPVSVKISFEDEPRKKYVDAETSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
92
Chicken
91
Xenopus
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6M7 As Substrate

Site PTM Type Enzyme
K23 Ubiquitination
S26 Phosphorylation
T30 Phosphorylation
K31 Ubiquitination
K34 Ubiquitination
S39 Phosphorylation
Y44 Phosphorylation
S52 Phosphorylation
S55 Phosphorylation
S76 Phosphorylation
S84 Phosphorylation
S86 Phosphorylation
Y87 Phosphorylation
T89 Phosphorylation
S91 Phosphorylation
T114 Phosphorylation
Y121 Phosphorylation
Y127 Phosphorylation
K130 Ubiquitination
T132 Phosphorylation
K137 Ubiquitination
S150 Phosphorylation
Y153 Phosphorylation
T156 Phosphorylation
S167 Phosphorylation
S181 Phosphorylation
T182 Phosphorylation
S197 Phosphorylation
S203 Phosphorylation
T225 Phosphorylation
S226 Phosphorylation
S233 Phosphorylation
K240 Ubiquitination
S242 Phosphorylation
S260 Phosphorylation
S266 Phosphorylation
S271 Phosphorylation
S297 Phosphorylation
S317 Phosphorylation
S382 Phosphorylation
S390 Phosphorylation
S392 Phosphorylation
S400 Phosphorylation
S403 Phosphorylation
S407 Phosphorylation
T408 Phosphorylation
S412 Phosphorylation
K421 Ubiquitination
S429 Phosphorylation
T459 Phosphorylation
Y481 Phosphorylation
S487 Phosphorylation
T490 Phosphorylation
T493 Phosphorylation
K503 Ubiquitination
K539 Ubiquitination
K546 Ubiquitination
K548 Ubiquitination
S556 Phosphorylation
T557 Phosphorylation
T559 Phosphorylation
T563 Phosphorylation
K590 Ubiquitination
T663 Phosphorylation
N776 N-Glycosylation
K784 Ubiquitination
N786 N-Glycosylation
N791 N-Glycosylation
K803 Ubiquitination
K854 Ubiquitination
T867 Phosphorylation
K1066 Ubiquitination
K1142 Ubiquitination
K1144 Acetylation
K1151 Acetylation
T1167 Phosphorylation
S1176 Phosphorylation
K1183 Ubiquitination
K1186 Ubiquitination
S1188 Phosphorylation
S1194 Phosphorylation
S1198 Phosphorylation
K1206 Ubiquitination
Y1207 Phosphorylation
S1213 Phosphorylation

Research Backgrounds

Function:

Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na(+):HCO3(-) 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity.

Subcellular Location:

Basolateral cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Cell projection>Stereocilium.
Note: Also described at the apical cell membrane. Localizes to the stereocilia of cochlear outer hair cells and to the lateral membrane of cochlear inner hair cells (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis and spleen. Also expressed in retina, colon, small intestine, ovary, thymus, prostate, muscle, heart and kidney. Isoform 1 is expressed in skeletal muscle and heart muscle.

Subunit Structure:

Interacts with CFTR through SLC9A3R1/EBP50. Interacts with USH1C. Forms a complex with ATP6V1B1 and SLC9A3R1/EBP50. Interacts in a pH dependent-manner with CA2/carbonic anhydrase 2.

Family&Domains:

The PDZ-binding motif mediates interaction with the CFTR, SLC9A3R1/EBP50 complex and probably with USH1C.

Belongs to the anion exchanger (TC 2.A.31) family.

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