SH3PXD2A Antibody - #DF9902

(4)  
Product: SH3PXD2A Antibody
Catalog: DF9902
Description: Rabbit polyclonal antibody to SH3PXD2A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 125 kDa; 125kD(Calculated).
Uniprot: Q5TCZ1
RRID: AB_2843096

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SH3PXD2A Antibody detects endogenous levels of total SH3PXD2A.
RRID:
AB_2843096
Cite Format: Affinity Biosciences Cat# DF9902, RRID:AB_2843096.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adapter protein TKS5; Five SH3 domain-containing protein; SH3 and PX domain-containing protein 2A; SH3 multiple domains protein 1; Sh3md1; Sh3pxd2a; SPD2A_HUMAN; TKs5; Tyrosine kinase substrate with five SH3 domains;

Immunogens

Immunogen:
Uniprot:

Q5TCZ1(SPD2A_HUMAN) >>Visit HPA database.

Gene(ID):
SH3PXD2A(9644)
Expression:
Q5TCZ1 SPD2A_HUMAN:

Found in several cancer cell lines, particularly invasive breast carcinomas and melanomas.

Sequence:
MLAYCVQDATVVDVEKRRNPSKHYVYIINVTWSDSTSQTIYRRYSKFFDLQMQLLDKFPIEGGQKDPKQRIIPFLPGKILFRRSHIRDVAVKRLKPIDEYCRALVRLPPHISQCDEVFRFFEARPEDVNPPKEDYGSSKRKSVWLSSWAESPKKDVTGADATAEPMILEQYVVVSNYKKQENSELSLQAGEVVDVIEKNESGWWFVSTSEEQGWVPATYLEAQNGTRDDSDINTSKTGEVSKRRKAHLRRLDRRWTLGGMVNRQHSREEKYVTVQPYTSQSKDEIGFEKGVTVEVIRKNLEGWWYIRYLGKEGWAPASYLKKAKDDLPTRKKNLAGPVEIIGNIMEISNLLNKKASGDKETPPAEGEGHEAPIAKKEISLPILCNASNGSAVGVPDRTVSRLAQGSPAVARIAPQRAQISSPNLRTRPPPRRESSLGFQLPKPPEPPSVEVEYYTIAEFQSCISDGISFRGGQKAEVIDKNSGGWWYVQIGEKEGWAPASYIDKRKKPNLSRRTSTLTRPKVPPPAPPSKPKEAEEGPTGASESQDSPRKLKYEEPEYDIPAFGFDSEPELSEEPVEDRASGERRPAQPHRPSPASSLQRARFKVGESSEDVALEEETIYENEGFRPYAEDTLSARGSSGDSDSPGSSSLSLTRKNSPKSGSPKSSSLLKLKAEKNAQAEMGKNHSSASFSSSITINTTCCSSSSSSSSSLSKTSGDLKPRSASDAGIRGTPKVRAKKDADANAGLTSCPRAKPSVRPKPFLNRAESQSQEKMDISTLRRQLRPTGQLRGGLKGSKSEDSELPPQTASEAPSEGSRRSSSDLITLPATTPPCPTKKEWEGPATSYMTCSAYQKVQDSEISFPAGVEVQVLEKQESGWWYVRFGELEGWAPSHYLVLDENEQPDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNTVNQSKKATPPIPSKPPGGFGKTSGTPAVKMRNGVRQVAVRPQSVFVSPPPKDNNLSCALRRNESLTATDGLRGVRRNSSFSTARSAAAEAKGRLAERAASQGSDSPLLPAQRNSIPVSPVRPKPIEKSQFIHNNLKDVYVSIADYEGDEETAGFQEGVSMEVLERNPNGWWYCQILDGVKPFKGWVPSNYLEKKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Dog
88
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5TCZ1 As Substrate

Site PTM Type Enzyme
K78 Ubiquitination
S84 Phosphorylation
K236 Ubiquitination
T256 Phosphorylation
K282 Ubiquitination
Y305 Phosphorylation
Y308 Phosphorylation
Y319 Phosphorylation
K321 Ubiquitination
T361 Phosphorylation
S379 Phosphorylation
S406 Phosphorylation
S420 Phosphorylation
S421 Phosphorylation
Y453 Phosphorylation
S468 Phosphorylation
K504 Ubiquitination
S515 Phosphorylation
T518 Phosphorylation
S547 Phosphorylation
K552 Ubiquitination
Y553 Phosphorylation
Y558 Phosphorylation
S567 Phosphorylation
S593 Phosphorylation
S596 Phosphorylation
S597 Phosphorylation
S608 Phosphorylation
S638 Phosphorylation
S642 Phosphorylation
S644 Phosphorylation
S648 Phosphorylation
S651 Phosphorylation
S662 Phosphorylation
S665 Phosphorylation
S666 Phosphorylation
S704 Phosphorylation
S705 Phosphorylation
S706 Phosphorylation
S707 Phosphorylation
S724 Phosphorylation
T731 Phosphorylation
S748 Phosphorylation
C749 S-Nitrosylation
S767 Phosphorylation
S769 Phosphorylation
S776 Phosphorylation
T785 Phosphorylation
R789 Methylation
S797 Phosphorylation
S818 Phosphorylation
S819 Phosphorylation
T828 Phosphorylation
T829 Phosphorylation
K915 Acetylation
K915 Ubiquitination
K922 Acetylation
S925 Phosphorylation
K928 Ubiquitination
K943 Ubiquitination
T946 Phosphorylation
K959 Methylation
S981 Phosphorylation
S985 Phosphorylation
S1002 Phosphorylation
T1004 Phosphorylation
R1010 Methylation
S1016 Phosphorylation
S1017 Phosphorylation
S1038 Phosphorylation
S1043 Phosphorylation
S1052 Phosphorylation
S1056 Phosphorylation

Research Backgrounds

Function:

Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.

PTMs:

Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein localization. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on serine upon DNA damage, probably by ATM or ATR.

Subcellular Location:

Cytoplasm. Cell projection>Podosome.
Note: Cytoplasmic in normal cells and localizes to podosomes in SRC-transformed cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in several cancer cell lines, particularly invasive breast carcinomas and melanomas.

Subunit Structure:

Interacts (via N-terminus) with CYBA (By similarity). Interacts with ADAM12, ADAM15 and ADAM19. Interacts with NOXO1. Interacts (via SH3 domains) with NOXA1. Interacts with FASLG.

Family&Domains:

The PX domain is required for podosome localization because of its ability to bind phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) and, to a lesser extent, phosphatidylinositol 4-phosphate (PtdIns(4)P), phosphatidylinositol 5-phosphate (PtdIns(5)P), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Binds to the third intramolecular SH3 domain (By similarity).

The fifth SH3 domain mediates binding with ADAM12, ADAM15 and ADAM19.

Belongs to the SH3PXD2 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.