NEK4 Antibody - #DF9886

(2)  
Product: NEK4 Antibody
Catalog: DF9886
Description: Rabbit polyclonal antibody to NEK4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 95 kDa; 95kD(Calculated).
Uniprot: P51957
RRID: AB_2843080

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(82%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
NEK4 Antibody detects endogenous levels of total NEK4.
RRID:
AB_2843080
Cite Format: Affinity Biosciences Cat# DF9886, RRID:AB_2843080.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.7.11.1; MGC33171; Never in mitosis A related kinase 4; Never in mitosis gene A related kinase 4; NIMA (never in mitosis gene a) related kinase 4; NIMA (never in mitosis gene a)-related expressed kinase 4; NIMA related kinase 4; NimA related protein kinase 4; NLK2; NRK2; OTTHUMP00000213582; Pp12301; Serine/threonine kinase 2; Serine/threonine protein kinase 2; Serine/threonine protein kinase Nek4; Serine/threonine protein kinase NRK2; STK2;

Immunogens

Immunogen:
Uniprot:

P51957(NEK4_HUMAN) >>Visit HPA database.

Gene(ID):
NEK4(6787)
Expression:
P51957 NEK4_HUMAN:

Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, testis, retina, liver, kidney, lung and placenta. Present in most primary carcinomas.

Sequence:
MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNNIKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCKQDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQPENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASRVAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMNF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Bovine
91
Sheep
91
Rabbit
91
Pig
82
Dog
82
Xenopus
80
Chicken
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51957 As Substrate

Site PTM Type Enzyme
R10 Methylation
K14 Ubiquitination
K24 Ubiquitination
K30 Ubiquitination
S43 Phosphorylation
S44 Phosphorylation
K61 Ubiquitination
K133 Ubiquitination
K146 Ubiquitination
K181 Ubiquitination
K209 Ubiquitination
Y216 Phosphorylation
K222 Ubiquitination
S243 Phosphorylation
S266 Phosphorylation
K273 Acetylation
K275 Acetylation
K278 Acetylation
K289 Ubiquitination
Y319 Phosphorylation
K339 Methylation
K339 Ubiquitination
S340 Phosphorylation
S343 Phosphorylation
K345 Methylation
K345 Ubiquitination
S377 Phosphorylation
K442 Ubiquitination
S461 Phosphorylation
S474 Phosphorylation
S481 Phosphorylation
S482 Phosphorylation
S484 Phosphorylation
S487 Phosphorylation
S489 Phosphorylation
S526 Phosphorylation
S533 Phosphorylation
S563 Phosphorylation
T580 Phosphorylation
T582 Phosphorylation
S608 Phosphorylation
K609 Ubiquitination
K622 Methylation
K622 Ubiquitination
S629 Phosphorylation
S630 Phosphorylation
S631 Phosphorylation
S634 Phosphorylation
K637 Ubiquitination
S639 Phosphorylation
K647 Acetylation
K647 Ubiquitination
S661 Phosphorylation
S662 Phosphorylation
S677 Phosphorylation
K690 Ubiquitination
S691 Phosphorylation
K699 Ubiquitination
K721 Ubiquitination
K737 Ubiquitination
K751 Ubiquitination
K762 Ubiquitination
S772 Phosphorylation
K828 Ubiquitination
K833 Ubiquitination

Research Backgrounds

Function:

Protein kinase that seems to act exclusively upon threonine residues (By similarity). Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage.

Subcellular Location:

Cell projection>Cilium. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, testis, retina, liver, kidney, lung and placenta. Present in most primary carcinomas.

Subunit Structure:

Interacts with RPGRIP1 and RPGRIP1L.

Family&Domains:

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.