CROCC Antibody | Affinity Biosciences

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Western blot analysis of extracts from mouse brain, using CROCC Antibody.

Product:	CROCC Antibody
Catalog:	DF9876
Description:	Rabbit polyclonal antibody to CROCC
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Sheep, Dog
Mol.Wt.:	229 kDa; 229kD(Calculated).
Uniprot:	Q5TZA2
RRID:	AB_2843070

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(89%), Sheep(80%), Dog(90%)

Clonality:

Polyclonal

Specificity:

CROCC Antibody detects endogenous levels of total CROCC.

RRID:

AB_2843070
Cite Format: Affinity Biosciences Cat# DF9876, RRID:AB_2843070.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ciliary rootlet coiled coil; Ciliary rootlet coiled-coil protein; Crocc; CROCC_HUMAN; KIAA0445; Rootletin;

Immunogens

Immunogen:

Uniprot:

Q5TZA2(CROCC_HUMAN) >>Visit HPA database.

Gene(ID):

CROCC(9696)

Sequence:

Q5TZA2 CROCC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLGLARAQEVELTLETVIQTLESSVLCQEKGLGARDLAQDAQITSLPALIREIVTRNLSQPESPVLLPATEMASLLSLQEENQLLQQELSRVEDLLAQSRAERDELAIKYNAVSERLEQALRLEPGELETQEPRGLVRQSVELRRQLQEEQASYRRKLQAYQEGQQRQAQLVQRLQGKILQYKKRCSELEQQLLERSGELEQQRLRDTEHSQDLESALIRLEEEQQRSASLAQVNAMLREQLDQAGSANQALSEDIRKVTNDWTRCRKELEHREAAWRREEESFNAYFSNEHSRLLLLWRQVVGFRRLVSEVKMFTERDLLQLGGELARTSRAVQEAGLGLSTGLRLAESRAEAALEKQALLQAQLEEQLRDKVLREKDLAQQQMQSDLDKADLSARVTELGLAVKRLEKQNLEKDQVNKDLTEKLEALESLRLQEQAALETEDGEGLQQTLRDLAQAVLSDSESGVQLSGSERTADASNGSLRGLSGQRTPSPPRRSSPGRGRSPRRGPSPACSDSSTLALIHSALHKRQLQVQDMRGRYEASQDLLGTLRKQLSDSESERRALEEQLQRLRDKTDGAMQAHEDAQREVQRLRSANELLSREKSNLAHSLQVAQQQAEELRQEREKLQAAQEELRRQRDRLEEEQEDAVQDGARVRRELERSHRQLEQLEGKRSVLAKELVEVREALSRATLQRDMLQAEKAEVAEALTKAEAGRVELELSMTKLRAEEASLQDSLSKLSALNESLAQDKLDLNRLVAQLEEEKSALQGRQRQAEQEATVAREEQERLEELRLEQEVARQGLEGSLRVAEQAQEALEQQLPTLRHERSQLQEQLAQLSRQLSGREQELEQARREAQRQVEALERAAREKEALAKEHAGLAVQLVAAEREGRTLSEEATRLRLEKEALEGSLFEVQRQLAQLEARREQLEAEGQALLLAKETLTGELAGLRQQIIATQEKASLDKELMAQKLVQAEREAQASLREQRAAHEEDLQRLQREKEAAWRELEAERAQLQSQLQREQEELLARLEAEKEELSEEIAALQQERDEGLLLAESEKQQALSLKESEKTALSEKLMGTRHSLATISLEMERQKRDAQSRQEQDRSTVNALTSELRDLRAQREEAAAAHAQEVRRLQEQARDLGKQRDSCLREAEELRTQLRLLEDARDGLRRELLEAQRKLRESQEGREVQRQEAGELRRSLGEGAKEREALRRSNEELRSAVKKAESERISLKLANEDKEQKLALLEEARTAVGKEAGELRTGLQEVERSRLEARRELQELRRQMKMLDSENTRLGRELAELQGRLALGERAEKESRRETLGLRQRLLKGEASLEVMRQELQVAQRKLQEQEGEFRTRERRLLGSLEEARGTEKQQLDHARGLELKLEAARAEAAELGLRLSAAEGRAQGLEAELARVEVQRRAAEAQLGGLRSALRRGLGLGRAPSPAPRPVPGSPARDAPAEGSGEGLNSPSTLECSPGSQPPSPGPATSPASPDLDPEAVRGALREFLQELRSAQRERDELRTQTSALNRQLAEMEAERDSATSRARQLQKAVAESEEARRSVDGRLSGVQAELALQEESVRRSERERRATLDQVATLERSLQATESELRASQEKISKMKANETKLEGDKRRLKEVLDASESRTVKLELQRRSLEGELQRSRLGLSDREAQAQALQDRVDSLQRQVADSEVKAGTLQLTVERLNGALAKVEESEGALRDKVRGLTEALAQSSASLNSTRDKNLHLQKALTACEHDRQVLQERLDAARQALSEARKQSSSLGEQVQTLRGEVADLELQRVEAEGQLQQLREVLRQRQEGEAAALNTVQKLQDERRLLQERLGSLQRALAQLEAEKREVERSALRLEKDRVALRRTLDKVEREKLRSHEDTVRLSAEKGRLDRTLTGAELELAEAQRQIQQLEAQVVVLEQSHSPAQLEVDAQQQQLELQQEVERLRSAQAQTERTLEARERAHRQRVRGLEEQVSTLKGQLQQELRRSSAPFSPPSGPPEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Sheep

Bovine

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5TZA2 As Substrate

Q5TZA2

Site	PTM Type	Enzyme	Source
S154	Phosphorylation		Uniprot
S284	Phosphorylation		Uniprot
Y288	Phosphorylation		Uniprot
K379	Ubiquitination		Uniprot
T400	Phosphorylation		Uniprot
K426	Ubiquitination		Uniprot
S462	Phosphorylation		Uniprot
S483	Phosphorylation		Uniprot
S488	Phosphorylation		Uniprot
T492	Phosphorylation		Uniprot
S494	Phosphorylation		Uniprot
S500	Phosphorylation		Uniprot
S506	Phosphorylation		Uniprot
S512	Phosphorylation		Uniprot
S516	Phosphorylation		Uniprot
T520	Phosphorylation		Uniprot
K712	Acetylation		Uniprot
T725	Phosphorylation		Uniprot
S807	Phosphorylation		Uniprot
S830	Phosphorylation		Uniprot
S844	Phosphorylation		Uniprot
S1151	Phosphorylation		Uniprot
S1218	Phosphorylation		Uniprot
K1259	Ubiquitination		Uniprot
K1290	Ubiquitination		Uniprot
K1390	Ubiquitination		Uniprot
R1442	Methylation		Uniprot
S1451	Phosphorylation		Uniprot
S1460	Phosphorylation	P24941 (CDK2)	Uniprot
S1476	Phosphorylation		Uniprot
S1490	Phosphorylation		Uniprot
S1587	Phosphorylation		Uniprot
T1598	Phosphorylation		Uniprot
S1619	Phosphorylation		Uniprot
K1622	Ubiquitination		Uniprot
S1647	Phosphorylation		Uniprot
S1660	Phosphorylation		Uniprot
R1685	Methylation		Uniprot
T1832	Phosphorylation		Uniprot
S1849	Phosphorylation		Uniprot
S1892	Phosphorylation		Uniprot
S1900	Phosphorylation		Uniprot
T1909	Phosphorylation		Uniprot
T1911	Phosphorylation		Uniprot
S2005	Phosphorylation		Uniprot
S2009	Phosphorylation		Uniprot
S2012	Phosphorylation		Uniprot

Research Backgrounds

Q5TZA2_CROCC_HUMAN

Function:

Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus (By similarity). Furthermore, is required for the correct positioning of the cilium basal body relative to the cell nucleus, to allow for ciliogenesis. Contributes to centrosome cohesion before mitosis.

PTMs:

Phosphorylated by NEK2 which may regulate its association with centrosomes.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase.

Subunit Structure:

Homopolymer. Interacts with KLC3, NEK2 and the N-terminus of CEP250.

Family&Domains:

Belongs to the rootletin family.

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CROCC Antibody - #DF9876