RPAP3 Antibody - #DF9875
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9875, RRID:AB_2843069.
Fold/Unfold
FLJ21908; RNA polymerase II associated protein 3; RNA polymerase II-associated protein 3; rpap3; RPAP3_HUMAN; Spag;
Immunogens
- Q9H6T3 RPAP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSANKAIELQLQVKQNAEELQDFMRDLENWEKDIKQKDMELRRQNGVPEENLPPIRNGNFRKKKKGKAKESSKKTREENTKNRIKSYDYEAWAKLDVDRILDELDKDDSTHESLSQESESEEDGIHVDSQKALVLKEKGNKYFKQGKYDEAIDCYTKGMDADPYNPVLPTNRASAYFRLKKFAVAESDCNLAVALNRSYTKAYSRRGAARFALQKLEEAKKDYERVLELEPNNFEATNELRKISQALASKENSYPKEADIVIKSTEGERKQIEAQQNKQQAISEKDRGNGFFKEGKYERAIECYTRGIAADGANALLPANRAMAYLKIQKYEEAEKDCTQAILLDGSYSKAFARRGTARTFLGKLNEAKQDFETVLLLEPGNKQAVTELSKIKKELIEKGHWDDVFLDSTQRQNVVKPIDNPPHPGSTKPLKKVIIEETGNLIQTIDVPDSTTAAAPENNPINLANVIAATGTTSKKNSSQDDLFPTSDTPRAKVLKIEEVSDTSSLQPQASLKQDVCQSYSEKMPIEIEQKPAQFATTVLPPIPANSFQLESDFRQLKSSPDMLYQYLKQIEPSLYPKLFQKNLDPDVFNQIVKILHDFYIEKEKPLLIFEILQRLSELKRFDMAVMFMSETEKKIARALFNHIDKSGLKDSSVEELKKRYGG
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H6T3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| T81 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| Y88 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| T111 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| Y165 | Phosphorylation | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| R198 | Methylation | Uniprot | |
| K216 | Acetylation | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K271 | Acetylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| R307 | Methylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| T488 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| T491 | Phosphorylation | Uniprot | |
| K498 | Sumoylation | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| Y567 | Phosphorylation | Uniprot | |
| K607 | Acetylation | Uniprot |
Research Backgrounds
Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation.
Tightly associated with the RNA polymerase II complex. Component of the R2TP complex composed at least of PIHD1, RUVBL1, RUVBL2 and RPAP3. Interacts with PIH1D1. Interacts with TSC1 and TSC2. Interacts with PRPF8 and EFTUD2 in a ZNHIT2-dependent manner.
Belongs to the RPAP3 family.
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