RTKN Antibody - #DF9868

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Product: RTKN Antibody
Catalog: DF9868
Description: Rabbit polyclonal antibody to RTKN
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 63 kDa; 63kD(Calculated).
Uniprot: Q9BST9
RRID: AB_2843062

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
RTKN Antibody detects endogenous levels of total RTKN.
RRID:
AB_2843062
Cite Format: Affinity Biosciences Cat# DF9868, RRID:AB_2843062.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Rhotekin; Rtkn; RTKN_HUMAN; RTKN1;

Immunogens

Immunogen:
Uniprot:

Q9BST9(RTKN_HUMAN) >>Visit HPA database.

Gene(ID):
RTKN(6242)
Expression:
Q9BST9 RTKN_HUMAN:

Highly expressed in prostate, moderately in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle and peripheral blood leukocytes, and weakly in ovary, colon and thymus. Weakly expressed in all normal cell lines tested. Overexpressed in various cancer cell lines.

Sequence:
MFSRNHRSRVTVARGSALEMEFKRGRFRLSLFSDLPEDTELQRKLDHEIRMREGACKLLAACSQREQALEATKSLLVCNSRILSYMGELQRRKEAQVLGKTSRRPSDSGPPAERSPCRGRVCISDLRIPLMWKDTEYFKNKGDLHRWAVFLLLQLGEHIQDTEMILVDRTLTDISFQSNVLFAEAGPDFELRLELYGACVEEEGALTGGPKRLATKLSSSLGRSSGRRVRASLDSAGGSGSSPILLPTPVVGGPRYHLLAHTTLTLAAVQDGFRTHDLTLASHEENPAWLPLYGSVCCRLAAQPLCMTQPTASGTLRVQQAGEMQNWAQVHGVLKGTNLFCYRQPEDADTGEEPLLTIAVNKETRVRAGELDQALGRPFTLSISNQYGDDEVTHTLQTESREALQSWMEALWQLFFDMSQWKQCCDEIMKIETPAPRKPPQALAKQGSLYHEMAIEPLDDIAAVTDILTQREGARLETPPPWLAMFTDQPALPNPCSPASVAPAPDWTHPLPWGRPRTFSLDAVPPDHSPRARSVAPLPPQRSPRTRGLCSKGQPRTWLQSPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
92
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BST9 As Substrate

Site PTM Type Enzyme
R9 Methylation
T11 Phosphorylation
R14 Methylation
S30 Phosphorylation
S33 Phosphorylation
K57 Ubiquitination
S63 Phosphorylation
K93 Ubiquitination
K100 Ubiquitination
S106 Phosphorylation
S108 Phosphorylation
S115 Phosphorylation
K133 Ubiquitination
K139 Ubiquitination
K216 Ubiquitination
S218 Phosphorylation
S219 Phosphorylation
S220 Phosphorylation
S232 Phosphorylation
S235 Phosphorylation
S239 Phosphorylation
S241 Phosphorylation
S242 Phosphorylation
T433 Phosphorylation
S448 Phosphorylation Q9BZL6 (PRKD2) , Q15139 (PRKD1) , O94806 (PRKD3)
Y450 Phosphorylation
T465 Phosphorylation
T518 Phosphorylation
S520 Phosphorylation
S529 Phosphorylation
R531 Methylation
R533 Methylation
S543 Phosphorylation
R547 Methylation

Research Backgrounds

Function:

Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures.

Tissue Specificity:

Highly expressed in prostate, moderately in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle and peripheral blood leukocytes, and weakly in ovary, colon and thymus. Weakly expressed in all normal cell lines tested. Overexpressed in various cancer cell lines.

Subunit Structure:

Interacts via its C-terminal region with the TAX1BP3 PDZ domain. This interaction facilitates Rho-mediated activation of the c-Fos serum response element (SRE). Interacts with SEPT9. Specifically binds to GTP-bound RHOA, RHOB and RHOC and inhibits their GTPase activity.

Restrictive clause

 

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For Research Use Only.
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