RHG30 Antibody - #DF9861

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Product: RHG30 Antibody
Catalog: DF9861
Description: Rabbit polyclonal antibody to RHG30
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 119 kDa; 119kD(Calculated).
Uniprot: Q7Z6I6
RRID: AB_2843055

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RHG30 Antibody detects endogenous levels of total RHG30.
RRID:
AB_2843055
Cite Format: Affinity Biosciences Cat# DF9861, RRID:AB_2843055.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Arhgap30; FLJ00267; FLJ44128; OTTHUMP00000029705; RHG30_HUMAN; Rho GTPase activating protein 30; Rho GTPase-activating protein 30; Rho type GTPase activating protein 30; Rho-type GTPase-activating protein 30; RP11-544M22.6;

Immunogens

Immunogen:
Uniprot:

Q7Z6I6(RHG30_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGAP30(257106)
Sequence:
MKSRQKGKKKGSAKERVFGCDLQEHLQHSGQEVPQVLKSCAEFVEEYGVVDGIYRLSGVSSNIQKLRQEFESERKPDLRRDVYLQDIHCVSSLCKAYFRELPDPLLTYRLYDKFAEAVGVQLEPERLVKILEVLRELPVPNYRTLEFLMRHLVHMASFSAQTNMHARNLAIVWAPNLLRSKDIEASGFNGTAAFMEVRVQSIVVEFILTHVDQLFGGAALSGGEVESGWRSLPGTRASGSPEDLMPRPLPYHLPSILQAGDGPPQMRPYHTIIEIAEHKRKGSLKVRKWRSIFNLGRSGHETKRKLPRGAEDREDKSNKGTLRPAKSMDSLSAAAGASDEPEGLVGPSSPRPSPLLPESLENDSIEAAEGEQEPEAEALGGTNSEPGTPRAGRSAIRAGGSSRAERCAGVHISDPYNVNLPLHITSILSVPPNIISNVSLARLTRGLECPALQHRPSPASGPGPGPGLGPGPPDEKLEASPASSPLADSGPDDLAPALEDSLSQEVQDSFSFLEDSSSSEPEWVGAEDGEVAQAEAAGAAFSPGEDDPGMGYLEELLGVGPQVEEFSVEPPLDDLSLDEAQFVLAPSCCSLDSAGPRPEVEEENGEEVFLSAYDDLSPLLGPKPPIWKGSGSLEGEAAGCGRQALGQGGEEQACWEVGEDKQAEPGGRLDIREEAEGSPETKVEAGKASEDRGEAGGSQETKVRLREGSREETEAKEEKSKGQKKADSMEAKGVEEPGGDEYTDEKEKEIEREEDEQREEAQVEAGRDLEQGAQEDQVAEEKWEVVQKQEAEGVREDEDKGQREKGYHEARKDQGDGEDSRSPEAATEGGAGEVSKERESGDGEAEGDQRAGGYYLEEDTLSEGSGVASLEVDCAKEGNPHSSEMEEVAPQPPQPEEMEPEGQPSPDGCLCPCSLGLGGVGMRLASTLVQVQQVRSVPVVPPKPQFAKMPSAMCSKIHVAPANPCPRPGRLDGTPGERAWGSRASRSSWRNGGSLSFDAAVALARDRQRTEAQGVRRTQTCTEGGDYCLIPRTSPCSMISAHSPRPLSCLELPSEGAEGSGSRSRLSLPPREPQVPDPLLSSQRRSYAFETQANPGKGEGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
78
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z6I6 As Substrate

Site PTM Type Enzyme
K65 Ubiquitination
K95 Ubiquitination
K129 Ubiquitination
K181 Ubiquitination
S240 Phosphorylation
Y269 Phosphorylation
S283 Phosphorylation
S291 Phosphorylation
T302 Phosphorylation
T321 Phosphorylation
S327 Phosphorylation
S330 Phosphorylation
S384 Phosphorylation
T388 Phosphorylation
S394 Phosphorylation
S401 Phosphorylation
S402 Phosphorylation
S413 Phosphorylation
S576 Phosphorylation
S617 Phosphorylation
S630 Phosphorylation
S632 Phosphorylation
S678 Phosphorylation
K702 Ubiquitination
S709 Phosphorylation
K725 Acetylation
Y742 Phosphorylation
K746 Acetylation
K836 Ubiquitination
S840 Phosphorylation
S862 Phosphorylation
S865 Phosphorylation
T927 Phosphorylation
S987 Phosphorylation
S988 Phosphorylation
S996 Phosphorylation
T1018 Phosphorylation
T1020 Phosphorylation
T1022 Phosphorylation
Y1027 Phosphorylation
S1043 Phosphorylation
S1048 Phosphorylation
S1082 Phosphorylation
S1086 Phosphorylation
Y1087 Phosphorylation

Research Backgrounds

Function:

GTPase-activating protein (GAP) for RAC1 and RHOA, but not for CDC42.

Subcellular Location:

Cytoplasmic vesicle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with RHOU in a GTP-independent manner.

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For Research Use Only.
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