Product: RHG28 Antibody
Catalog: DF9860
Description: Rabbit polyclonal antibody to RHG28
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 82 kDa; 82kD(Calculated).
Uniprot: Q9P2N2
RRID: AB_2843054

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(80%)
Clonality:
Polyclonal
Specificity:
RHG28 Antibody detects endogenous levels of total RHG28.
RRID:
AB_2843054
Cite Format: Affinity Biosciences Cat# DF9860, RRID:AB_2843054.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHGAP28; KIAA1314; Rho GTPase activating protein 28; Rho type GTPase activating protein 28;

Immunogens

Immunogen:
Uniprot:
Expression:
Q9P2N2 RHG28_HUMAN:

Expressed in testis. Expressed at moderate level in kidney and ovary, and weakly expressed in spleen and skeletal muscle.

Sequence:
MEVEDSGGVVLTAYHSYARAQPPNAESRCAPRAAASHPLSRKSIPRCRRINRMLSNESLHPPAFSRSNSEASVDSASMEDFWREIESIKDSSMGGQEEPPPAEVTPVDEGELEAEWLQDVGLSTLISGDEEEDGKALLSTLTRTQAAAVQKRYHTYTQTMRKKDKQSIRDVRDIFGVSESPPRDTCGNHTNQLDGTKEERELPRVIKTSGSMPDDASLNSTTLSDASQDKEGSFAVPRSDSVAILETIPVLPVHSNGSPEPGQPVQNAISDDDFLEKNIPPEAEELSFEVSYSEMVTEALKRNKLKKSEIKKEDYVLTKFNVQKTRFGLTEAGDLSAEDMKKIRHLSLIELTAFFDAFGIQLKRNKTEKVKGRDNGIFGVPLTVLLDGDRKKDPGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAKFNADKFKWDKMCHREAAVMLKAFFRELPTSLFPVEYIPAFISLMERGPHVKVQFQALHLMVMALPDANRDAAQALMTFFNKVIANESKNRMSLWNISTVMAPNLFFSRSKHSDYEELLLANTAAHIIRLMLKYQKILWKVPSFLITQVRRMNEATMLLKKQLPSVRKLLRRKTLERETASPKTSKVLQKSPSARRMSDVPEGVIRVHAPLLSKVSMAIQLNNQTKAKDILAKFQYENSHGSSECIKIQNQRLYEIGGNIGEHCLDPDAYILDVYRINPQAEWVIKPQQSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
80
Dog
77
Pig
73
Rabbit
73
Bovine
60
Sheep
60
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P2N2 As Substrate

Site PTM Type Enzyme
S36 Phosphorylation
S55 Phosphorylation
S65 Phosphorylation
S69 Phosphorylation
S139 Phosphorylation
T140 Phosphorylation
S178 Phosphorylation
S180 Phosphorylation
K207 Acetylation
S211 Phosphorylation
Y315 Phosphorylation
T318 Phosphorylation
S347 Phosphorylation
T352 Phosphorylation
S423 Phosphorylation
T516 Phosphorylation
Y553 Phosphorylation
T594 Phosphorylation
K628 Ubiquitination
S636 Phosphorylation
R644 Methylation
S680 Phosphorylation

Research Backgrounds

Function:

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

Tissue Specificity:

Expressed in testis. Expressed at moderate level in kidney and ovary, and weakly expressed in spleen and skeletal muscle.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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