RHG25 Antibody - #DF9859
(2)
| Product: | RHG25 Antibody |
| Catalog: | DF9859 |
| Description: | Rabbit polyclonal antibody to RHG25 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Horse, Rabbit |
| Mol.Wt.: | 73 kDa; 73kD(Calculated). |
| Uniprot: | P42331 |
| RRID: | AB_2843053 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Horse(89%), Rabbit(82%)
Clonality:
Polyclonal
Specificity:
RHG25 Antibody detects endogenous levels of total RHG25.
RRID:
AB_2843053
Cite Format: Affinity Biosciences Cat# DF9859, RRID:AB_2843053.
Cite Format: Affinity Biosciences Cat# DF9859, RRID:AB_2843053.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ARHGAP 25; ARHGAP25; Epididymis secretory protein Li 308; HEL S 308; KAIA0053; RHG25_HUMAN; Rho GTPase activating protein 25; Rho GTPase-activating protein 25; Rho type GTPase activating protein 25; Rho-type GTPase-activating protein 25;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- P42331 RHG25_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLKLPRNWDFNLKVEAAKIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIVKNWQQRYFVLRAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWDQNRMGQDSYVLMASSQAEMEEWVKFLRRVAGTPCGVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGRNEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELMKQLSILPRDNYSLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDIPLSPPAQKNDPKKAPVARSSVGWDATEDLRISRTDSFSSMTSDSDTTSPTGQQPSDAFPEDSSKVPREKPGDWKMQSRKRTQTLPNRKCFLTSAFQGANSSKMEIFKNEFWSPSSEAKAGEGHRRTMSQDLRQLSDSQRTSTYDNVPSLPGSPGEEASALSSQACDSKGDTLASPNSETGPGKKNSGEEEIDSLQRMVQELRKEIETQKQMYEEQIKNLEKENYDVWAKVVRLNEELEKEKKKSAALEISLRNMERSREDVEKRNKALEEEVKEFVKSMKEPKTEA
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
89
Rabbit
82
Bovine
67
Sheep
67
Pig
64
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P42331 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| T226 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| T400 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| T442 | Phosphorylation | Uniprot | |
| K461 | Acetylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| T485 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| Y571 | Phosphorylation | Uniprot | |
| Y583 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
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