RHG24 Antibody - #DF9858

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Product: RHG24 Antibody
Catalog: DF9858
Description: Rabbit polyclonal antibody to RHG24
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 84 kDa; 84kD(Calculated).
Uniprot: Q8N264
RRID: AB_2843052

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(85%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
RHG24 Antibody detects endogenous levels of total RHG24.
RRID:
AB_2843052
Cite Format: Affinity Biosciences Cat# DF9858, RRID:AB_2843052.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHGAP24; DKFZP564B1162; Filamin A associated RhoGAP; FILGAP; FLJ33877; p73; p73RhoGAP; RAC1 and CDC4 specific GTPase activating protein of 72kDa; RCGAP72; Rho GTPase activating protein 24; Rho type GTPase activating protein 24; RhoGAP of 73 kDa; Sarcoma antigen NY SAR 88;

Immunogens

Immunogen:
Uniprot:

Q8N264(RHG24_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGAP24(83478)
Expression:
Q8N264 RHG24_HUMAN:

Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells.

Sequence:
MEENNDSTENPQQGQGRQNAIKCGWLRKQGGFVKTWHTRWFVLKGDQLYYFKDEDETKPLGTIFLPGNKVSEHPCNEENPGKFLFEVVPGGDRDRMTANHESYLLMASTQNDMEDWVKSIRRVIWGPFGGGIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVIPYAKYEDFLSCAKLLSKEEEAGVKELAKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPNILRPKVEDPLTIMEGTVVVQQLMSVMISKHDCLFPKDAELQSKPQDGVSNNNEIQKKATMGQLQNKENNNTKDSPSRQCSWDKSESPQRSSMNNGSPTALSGSKTNSPKNSVHKLDVSRSPPLMVKKNPAFNKGSGIVTNGSFSSSNAEGLEKTQTTPNGSLQARRSSSLKVSGTKMGTHSVQNGTVRMGILNSDTLGNPTNVRNMSWLPNGYVTLRDNKQKEQAGELGQHNRLSTYDNVHQQFSMMNLDDKQSIDSATWSTSSCEISLPENSNSCRSSTTTCPEQDFFGGNFEDPVLDGPPQDDLSHPRDYESKSDHRSVGGRSSRATSSSDNSETFVGNSSSNHSALHSLVSSLKQEMTKQKIEYESRIKSLEQRNLTLETEMMSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVEPRRTERGNTIWIQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Sheep
92
Dog
92
Rabbit
92
Bovine
85
Pig
77
Chicken
69
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N264 As Substrate

Site PTM Type Enzyme
T62 Phosphorylation
T354 Phosphorylation
S369 Phosphorylation
S371 Phosphorylation
S385 Phosphorylation
S391 Phosphorylation Q13464 (ROCK1)
T393 Phosphorylation
S398 Phosphorylation
S402 Phosphorylation Q13464 (ROCK1)
S406 Phosphorylation
S413 Phosphorylation Q13464 (ROCK1)
S415 Phosphorylation Q13464 (ROCK1)
K421 Acetylation
K422 Acetylation
S437 Phosphorylation Q13464 (ROCK1)
T452 Phosphorylation Q13464 (ROCK1)
S464 Phosphorylation
Y508 Phosphorylation
S573 Phosphorylation Q13464 (ROCK1)
S574 Phosphorylation Q13464 (ROCK1)
T575 Phosphorylation Q13464 (ROCK1)
T576 Phosphorylation Q13464 (ROCK1)
T577 Phosphorylation Q13464 (ROCK1)
T624 Phosphorylation
S627 Phosphorylation
S727 Phosphorylation
T728 Phosphorylation

Research Backgrounds

Function:

Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.

PTMs:

Phosphorylated by ROCK, leading to activate the RacGAP activity.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell junction>Adherens junction. Cell junction>Focal adhesion. Cell projection.
Note: Localizes to actin stress fibers. In migrating cells, localizes to membrane lamellae and protusions.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells.

Subunit Structure:

Interacts with FLNA.

Family&Domains:

The coiled coil domain mediates the interaction with FLNA leading to its recruitment to lamellae.

References

1). Aquaporin 4 deficiency eliminates the beneficial effects of voluntary exercise in a mouse model of Alzheimer’s disease. Neural Regeneration Research (PubMed: 35142700) [IF=6.1]
2). A Single-Nucleotide Polymorphism in the Promoter of Porcine ARHGAP24 Gene Regulates Aggressive Behavior of Weaned Pigs After Mixing by Affecting the Binding of Transcription Factor p53. Frontiers in Cell and Developmental Biology (PubMed: 35433684) [IF=5.5]

Application: WB    Species: Pig    Sample: neural cells

FIGURE 5 Transcription factor p53 regulates ARHGAP24 gene expression in porcine neural cells. (A) mRNA level of p53 after p53 overexpression. (B) mRNA level of p53 after p53 knockdown. (C) Luciferase activity of the ARHGAP24 promoter region after p53 overexpression (D) Luciferase activity of the ARHGAP24 promoter region after p53 knockdown (E) mRNA level of ARHGAP24 after p53 overexpression. (F) mRNA level of ARHGAP24 after p53 knockdown. (G) Western blot analyses of ARHGAP24 protein expression in porcine neural cells transfected with pcDNA3.1-p53 and pcDNA3.1 (+). (H) Western blot analyses of ARHGAP24 protein expression in porcine neural cells transfected with the scrambled and siRNA-p53 groups. The protein levels were normalized to GAPDH. *p < 0.05 and **p < 0.01. Different letters (a, b, c, etc.) before the columns indicate that the difference is significant (p < 0.05)

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