Product: RHG23 Antibody
Catalog: DF9857
Description: Rabbit polyclonal antibody to RHG23
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 162 kDa; 162kD(Calculated).
Uniprot: Q9P227
RRID: AB_2843051

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(82%), Dog(83%)
Clonality:
Polyclonal
Specificity:
RHG23 Antibody detects endogenous levels of total RHG23.
RRID:
AB_2843051
Cite Format: Affinity Biosciences Cat# DF9857, RRID:AB_2843051.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Arhgap23; KIAA1501; RHG23_HUMAN; Rho GTPase-activating protein 23; Rho-type GTPase-activating protein 23;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9P227 RHG23_HUMAN:

Expressed in placenta, prostate, hippocampus and brain medulla. Also expressed in brain tumor, salivary gland tumor, head and neck tumor.

Sequence:
MNGVAFCLVGIPPRPEPRPPQLPLGPRDGCSPRRPFPWQGPRTLLLYKSPQDGFGFTLRHFIVYPPESAVHCSLKEEENGGRGGGPSPRYRLEPMDTIFVKNVKEDGPAHRAGLRTGDRLVKVNGESVIGKTYSQVIALIQNSDDTLELSIMPKDEDILQLAYSQDAYLKGNEPYSGEARSIPEPPPICYPRKTYAPPARASTRATMVPEPTSALPSDPRSPAAWSDPGLRVPPAARAHLDNSSLGMSQPRPSPGAFPHLSSEPRTPRAFPEPGSRVPPSRLECQQALSHWLSNQVPRRAGERRCPAMAPRARSASQDRLEEVAAPRPWPCSTSQDALSQLGQEGWHRARSDDYLSRATRSAEALGPGALVSPRFERCGWASQRSSARTPACPTRDLPGPQAPPPSGLQGLDDLGYIGYRSYSPSFQRRTGLLHALSFRDSPFGGLPTFNLAQSPASFPPEASEPPRVVRPEPSTRALEPPAEDRGDEVVLRQKPPTGRKVQLTPARQMNLGFGDESPEPEASGRGERLGRKVAPLATTEDSLASIPFIDEPTSPSIDLQAKHVPASAVVSSAMNSAPVLGTSPSSPTFTFTLGRHYSQDCSSIKAGRRSSYLLAITTERSKSCDDGLNTFRDEGRVLRRLPNRIPSLRMLRSFFTDGSLDSWGTSEDADAPSKRHSTSDLSDATFSDIRREGWLYYKQILTKKGKKAGSGLRQWKRVYAALRARSLSLSKERREPGPAAAGAAAAGAGEDEAAPVCIGSCLVDISYSETKRRHVFRLTTADFCEYLFQAEDRDDMLGWIRAIRENSRAEGEDPGCANQALISKKLNDYRKVSHSSGPKADSSPKGSRGLGGLKSEFLKQSAARGLRTQDLPAGSKDDSAAAPKTPWGINIIKKNKKAAPRAFGVRLEECQPATENQRVPLIVAACCRIVEARGLESTGIYRVPGNNAVVSSLQEQLNRGPGDINLQDERWQDLNVISSLLKSFFRKLPEPLFTDDKYNDFIEANRIEDARERMRTLRKLIRDLPGHYYETLKFLVGHLKTIADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHSDWFFSDEEDKGERTPVGDKEPQAVPNIEYLLPNIGRTVPPGDPGSDSTTCSSAKSKGSWAPKKEPYAREMLAISFISAVNRKRKKRREARGLGSSTDDDSEQEAHKPGAGATAPGTQERPQGPLPGAVAPEAPGRLSPPAAPEERPAADTRSIVSGYSTLSTMDRSVCSGASGRRAGAGDEADDERSELSHVETDTEGAAGAGPGGRLTRRPSFSSHHLMPCDTLARRRLARGRPDGEGAGRGGPRAPEPPGSASSSSQESLRPPAAALASRPSRMEALRLRLRGTADDMLAVRLRRPLSPETRRRRSSWRRHTVVVQSPLTDLNFNEWKELGGGGPPEPAGARAHSDNKDSGLSSLESTKARAPSSAASQPPAPGDTGSLQSQPPRRSAASRLHQCL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
83
Rabbit
82
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P227 As Substrate

Site PTM Type Enzyme
Y90 Phosphorylation
K122 Ubiquitination
S127 Phosphorylation
Y163 Phosphorylation
S164 Phosphorylation
S181 Phosphorylation
T212 Phosphorylation
S213 Phosphorylation
S217 Phosphorylation
S221 Phosphorylation
S226 Phosphorylation
S261 Phosphorylation
S262 Phosphorylation
T266 Phosphorylation
S316 Phosphorylation
S334 Phosphorylation
S351 Phosphorylation
S361 Phosphorylation
S372 Phosphorylation
S423 Phosphorylation
S437 Phosphorylation
K500 Ubiquitination
S517 Phosphorylation
S554 Phosphorylation
S572 Phosphorylation
S576 Phosphorylation
T582 Phosphorylation
S583 Phosphorylation
S598 Phosphorylation
S602 Phosphorylation
S610 Phosphorylation
S611 Phosphorylation
Y612 Phosphorylation
T617 Phosphorylation
S621 Phosphorylation
S623 Phosphorylation
S647 Phosphorylation
T656 Phosphorylation
S659 Phosphorylation
S662 Phosphorylation
S673 Phosphorylation
K674 Ubiquitination
S677 Phosphorylation
S682 Phosphorylation
S687 Phosphorylation
K703 Acetylation
S726 Phosphorylation
S760 Phosphorylation
K824 Ubiquitination
S842 Phosphorylation
K854 Sumoylation
K854 Ubiquitination
K859 Methylation
K859 Ubiquitination
K876 Ubiquitination
K884 Ubiquitination
K987 Ubiquitination
K997 Ubiquitination
T1107 Phosphorylation
K1112 Ubiquitination
Y1122 Phosphorylation
T1130 Phosphorylation
S1138 Phosphorylation
T1141 Phosphorylation
T1142 Phosphorylation
S1144 Phosphorylation
S1145 Phosphorylation
T1189 Phosphorylation
S1230 Phosphorylation
T1243 Phosphorylation
S1306 Phosphorylation
S1308 Phosphorylation
S1309 Phosphorylation
T1317 Phosphorylation
R1335 Methylation
S1393 Phosphorylation
T1396 Phosphorylation
S1412 Phosphorylation
S1448 Phosphorylation
S1449 Phosphorylation
S1452 Phosphorylation
T1453 Phosphorylation
K1454 Ubiquitination
S1459 Phosphorylation
S1460 Phosphorylation
S1482 Phosphorylation

Research Backgrounds

Function:

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.

Tissue Specificity:

Expressed in placenta, prostate, hippocampus and brain medulla. Also expressed in brain tumor, salivary gland tumor, head and neck tumor.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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