RBBP6 Antibody - #DF9853

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Product: RBBP6 Antibody
Catalog: DF9853
Description: Rabbit polyclonal antibody to RBBP6
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 202 kDa; 202kD(Calculated).
Uniprot: Q7Z6E9
RRID: AB_2843047

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
RBBP6 Antibody detects endogenous levels of total RBBP6.
RRID:
AB_2843047
Cite Format: Affinity Biosciences Cat# DF9853, RRID:AB_2843047.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686P0638; DKFZp761B2423; E3 ubiquitin-protein ligase RBBP6; MY038; P2P R; P2PR; p53 associated cellular protein of testis; p53-associated cellular protein of testis; p53-associated cellular protein, testis-derived; PACT; Proliferation potential related protein; Proliferation potential-related protein; Protein P2P R; Protein P2P-R; Protein P2PR; Protein RBQ 1; Protein RBQ1; RB binding protein 6, ubiquitin ligase; RB binding Q protein 1; RBBP 6; Rbbp6; RBBP6_HUMAN; RBQ 1; RBQ-1; RBQ1; Retinoblastoma binding protein 6 isoform 3; Retinoblastoma binding Q protein 1; Retinoblastoma-binding protein 6; Retinoblastoma-binding Q protein 1; SNAMA; SNAMA, Drosophila, homolog of;

Immunogens

Immunogen:
Uniprot:

Q7Z6E9(RBBP6_HUMAN) >>Visit HPA database.

Gene(ID):
RBBP6(5930)
Expression:
Q7Z6E9 RBBP6_HUMAN:

Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.

Sequence:
MSCVHYKFSSKLNYDTVTFDGLHISLCDLKKQIMGREKLKAADCDLQITNAQTKEEYTDDNALIPKNSSVIVRRIPIGGVKSTSKTYVISRTEPAMATTKAIDDSSASISLAQLTKTANLAEANASEEDKIKAMMSQSGHEYDPINYMKKPLGPPPPSYTCFRCGKPGHYIKNCPTNGDKNFESGPRIKKSTGIPRSFMMEVKDPNMKGAMLTNTGKYAIPTIDAEAYAIGKKEKPPFLPEEPSSSSEEDDPIPDELLCLICKDIMTDAVVIPCCGNSYCDECIRTALLESDEHTCPTCHQNDVSPDALIANKFLRQAVNNFKNETGYTKRLRKQLPPPPPPIPPPRPLIQRNLQPLMRSPISRQQDPLMIPVTSSSTHPAPSISSLTSNQSSLAPPVSGNPSSAPAPVPDITATVSISVHSEKSDGPFRDSDNKILPAAALASEHSKGTSSIAITALMEEKGYQVPVLGTPSLLGQSLLHGQLIPTTGPVRINTARPGGGRPGWEHSNKLGYLVSPPQQIRRGERSCYRSINRGRHHSERSQRTQGPSLPATPVFVPVPPPPLYPPPPHTLPLPPGVPPPQFSPQFPPGQPPPAGYSVPPPGFPPAPANLSTPWVSSGVQTAHSNTIPTTQAPPLSREEFYREQRRLKEEEKKKSKLDEFTNDFAKELMEYKKIQKERRRSFSRSKSPYSGSSYSRSSYTYSKSRSGSTRSRSYSRSFSRSHSRSYSRSPPYPRRGRGKSRNYRSRSRSHGYHRSRSRSPPYRRYHSRSRSPQAFRGQSPNKRNVPQGETEREYFNRYREVPPPYDMKAYYGRSVDFRDPFEKERYREWERKYREWYEKYYKGYAAGAQPRPSANRENFSPERFLPLNIRNSPFTRGRREDYVGGQSHRSRNIGSNYPEKLSARDGHNQKDNTKSKEKESENAPGDGKGNKHKKHRKRRKGEESEGFLNPELLETSRKSREPTGVEENKTDSLFVLPSRDDATPVRDEPMDAESITFKSVSEKDKRERDKPKAKGDKTKRKNDGSAVSKKENIVKPAKGPQEKVDGERERSPRSEPPIKKAKEETPKTDNTKSSSSSQKDEKITGTPRKAHSKSAKEHQETKPVKEEKVKKDYSKDVKSEKLTTKEEKAKKPNEKNKPLDNKGEKRKRKTEEKGVDKDFESSSMKISKLEVTEIVKPSPKRKMEPDTEKMDRTPEKDKISLSAPAKKIKLNRETGKKIGSTENISNTKEPSEKLESTSSKVKQEKVKGKVRRKVTGTEGSSSTLVDYTSTSSTGGSPVRKSEEKTDTKRTVIKTMEEYNNDNTAPAEDVIIMIQVPQSKWDKDDFESEEEDVKSTQPISSVGKPASVIKNVSTKPSNIVKYPEKESEPSEKIQKFTKDVSHEIIQHEVKSSKNSASSEKGKTKDRDYSVLEKENPEKRKNSTQPEKESNLDRLNEQGNFKSLSQSSKEARTSDKHDSTRASSNKDFTPNRDKKTDYDTREYSSSKRRDEKNELTRRKDSPSRNKDSASGQKNKPREERDLPKKGTGDSKKSNSSPSRDRKPHDHKATYDTKRPNEETKSVDKNPCKDREKHVLEARNNKESSGNKLLYILNPPETQVEKEQITGQIDKSTVKPKPQLSHSSRLSSDLTRETDEAAFEPDYNESDSESNVSVKEEESSGNISKDLKDKIVEKAKESLDTAAVVQVGISRNQSHSSPSVSPSRSHSPSGSQTRSHSSSASSAESQDSKKKKKKKEKKKHKKHKKHKKHKKHAGTEVELEKSQKHKHKKKKSKKNKDKEKEKEKDDQKVKSVTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z6E9 As Substrate

Site PTM Type Enzyme
K7 Acetylation
K81 Ubiquitination
T86 Phosphorylation
Y87 Phosphorylation
K116 Ubiquitination
S126 Phosphorylation
K130 Acetylation
S138 Phosphorylation
Y142 Phosphorylation
R187 Methylation
S197 Phosphorylation
K208 Methylation
K208 Ubiquitination
T213 Phosphorylation
T215 Phosphorylation
Y218 Phosphorylation
T222 Phosphorylation
Y228 Phosphorylation
K232 Acetylation
S244 Phosphorylation
S245 Phosphorylation
S246 Phosphorylation
S247 Phosphorylation
K323 Sumoylation
S360 Phosphorylation
S363 Phosphorylation
S432 Phosphorylation
K435 Ubiquitination
T471 Phosphorylation
S516 Phosphorylation
R522 Methylation
R530 Methylation
R534 Methylation
K657 Sumoylation
K657 Ubiquitination
S682 Phosphorylation
S684 Phosphorylation
S688 Phosphorylation
Y690 Phosphorylation
S691 Phosphorylation
S693 Phosphorylation
S694 Phosphorylation
T701 Phosphorylation
S712 Phosphorylation
S714 Phosphorylation
S724 Phosphorylation
S726 Phosphorylation
S728 Phosphorylation
S730 Phosphorylation
S748 Phosphorylation
S750 Phosphorylation
S756 Phosphorylation
S758 Phosphorylation
S760 Phosphorylation
S768 Phosphorylation
S770 Phosphorylation
S772 Phosphorylation
S780 Phosphorylation
K809 Sumoylation
K809 Ubiquitination
S815 Phosphorylation
K824 Ubiquitination
Y841 Phosphorylation
K843 Ubiquitination
S854 Phosphorylation
S861 Phosphorylation
S873 Phosphorylation
T876 Phosphorylation
R877 Methylation
Y883 Phosphorylation
R892 Methylation
K901 Acetylation
K901 Ubiquitination
K941 Ubiquitination
S945 Phosphorylation
T956 Phosphorylation
S957 Phosphorylation
S960 Phosphorylation
K970 Ubiquitination
S979 Phosphorylation
T984 Phosphorylation
S1052 Phosphorylation
S1055 Phosphorylation
T1066 Phosphorylation
T1087 Phosphorylation
K1106 Sumoylation
K1109 Ubiquitination
K1122 Ubiquitination
T1173 Phosphorylation
S1179 Phosphorylation
T1194 Phosphorylation
S1203 Phosphorylation
T1215 Phosphorylation
S1221 Phosphorylation
T1222 Phosphorylation
S1226 Phosphorylation
T1228 Phosphorylation
S1237 Phosphorylation
T1238 Phosphorylation
S1239 Phosphorylation
S1240 Phosphorylation
T1256 Phosphorylation
Y1268 Phosphorylation
T1269 Phosphorylation
T1271 Phosphorylation
S1273 Phosphorylation
T1274 Phosphorylation
S1277 Phosphorylation
S1282 Phosphorylation
Y1299 Phosphorylation
S1328 Phosphorylation
T1336 Phosphorylation
S1341 Phosphorylation
K1344 Acetylation
K1344 Ubiquitination
S1347 Phosphorylation
S1353 Phosphorylation
S1381 Phosphorylation
K1400 Ubiquitination
T1403 Phosphorylation
Y1408 Phosphorylation
S1422 Phosphorylation
K1441 Methylation
S1442 Phosphorylation
S1444 Phosphorylation
S1463 Phosphorylation
T1468 Phosphorylation
S1500 Phosphorylation
K1531 Methylation
Y1549 Phosphorylation
T1551 Phosphorylation
S1560 Phosphorylation
K1571 Acetylation
S1622 Phosphorylation
S1625 Phosphorylation
S1626 Phosphorylation
Y1641 Phosphorylation
S1644 Phosphorylation
S1646 Phosphorylation
S1648 Phosphorylation
S1651 Phosphorylation
S1692 Phosphorylation
S1694 Phosphorylation
S1695 Phosphorylation
S1697 Phosphorylation
S1699 Phosphorylation
S1701 Phosphorylation
S1703 Phosphorylation
S1705 Phosphorylation
S1707 Phosphorylation
S1719 Phosphorylation
S1726 Phosphorylation
K1782 Acetylation
K1786 Acetylation
K1786 Ubiquitination
K1788 Acetylation

Research Backgrounds

Function:

E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth (By similarity). Regulates DNA-replication and the stability of chromosomal common fragile sites (CFSs) in a ZBTB38- and MCM10-dependent manner. Controls ZBTB38 protein stability and abundance via ubiquitination and proteasomal degradation, and ZBTB38 in turn negatively regulates the expression of MCM10 which plays an important role in DNA-replication.

PTMs:

Phosphorylated by NEK6.

Subcellular Location:

Nucleus>Nucleolus. Chromosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Colocalizes with mitotic chromosomes. Colocalizes with NEK6 in the centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.

Subunit Structure:

Interacts with p53/TP53 and RB1 (By similarity). Interacts also with MDM2 and YBX1. Interacts with NEK6. Interacts with ZBTB38.

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