RAB23 Antibody | Affinity Biosciences

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Western blot analysis of extracts from MDA-MB-231 cells, using RAB23 Antibody.

Product:	RAB23 Antibody
Catalog:	DF9821
Description:	Rabbit polyclonal antibody to RAB23
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	27 kDa; 27kD(Calculated).
Uniprot:	Q9ULC3
RRID:	AB_2843015

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(83%), Horse(82%), Sheep(83%), Rabbit(92%), Dog(83%)

Clonality:

Polyclonal

Specificity:

RAB23 Antibody detects endogenous levels of total RAB23.

RRID:

AB_2843015
Cite Format: Affinity Biosciences Cat# DF9821, RRID:AB_2843015.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp781H0695; HSPC137; MGC8900; Rab 23; RAB family small GTP binding protein RAB 23; Rab23; RAB23, member RAS oncogene family; RAB23_HUMAN; Ras related protein Rab 23; Ras-related protein Rab-23;

Immunogens

Immunogen:

Uniprot:

Q9ULC3(RAB23_HUMAN) >>Visit HPA database.

Gene(ID):

RAB23(51715)

Sequence:

Q9ULC3 RAB23_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLEEDMEVAIKMVVVGNGAVGKSSMIQRYCKGIFTKDYKKTIGVDFLERQIQVNDEDVRLMLWDTAGQEEFDAITKAYYRGAQACVLVFSTTDRESFEAVSSWREKVVAEVGDIPTVLVQNKIDLLDDSCIKNEEAEALAKRLKLRFYRTSVKEDLNVNEVFKYLAEKYLQKLKQQIAEDPELTHSSSNKIGVFNTSGGSHSGQNSGTLNGGDVINLRPNKQRTKKNRNPFSSCSIP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Bovine

Sheep

Dog

Horse

Xenopus

Chicken

Pig

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULC3 As Substrate

Q9ULC3

Site	PTM Type	Source
K122	Ubiquitination	Uniprot
K132	Ubiquitination	Uniprot
Y148	Phosphorylation	Uniprot
K163	Ubiquitination	Uniprot
Y164	Phosphorylation	Uniprot
K174	Ubiquitination	Uniprot
T184	Phosphorylation	Uniprot
S186	Phosphorylation	Uniprot
S187	Phosphorylation	Uniprot
T196	Phosphorylation	Uniprot
S200	Phosphorylation	Uniprot
S206	Phosphorylation	Uniprot
T208	Phosphorylation	Uniprot

Research Backgrounds

Q9ULC3_RAB23_HUMAN

Function:

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Together with SUFU, prevents nuclear import of GLI1, and thereby inhibits GLI1 transcription factor activity. Regulates GLI1 in differentiating chondrocytes. Likewise, regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity. Plays a role in autophagic vacuole assembly, and mediates defense against pathogens, such as S.aureus, by promoting their capture by autophagosomes that then merge with lysosomes.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side. Cytoplasm. Cytoplasmic vesicle>Autophagosome. Endosome membrane. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle>Phagosome membrane>Lipid-anchor>Cytoplasmic side.
Note: Recruited to phagosomes containing S.aureus or M.tuberculosis.

Subunit Structure:

Interacts with SUFU.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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RAB23 Antibody - #DF9821