RAPH1 Antibody - #DF9806

(3)  
Product: RAPH1 Antibody
Catalog: DF9806
Description: Rabbit polyclonal antibody to RAPH1
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Bovine, Rabbit, Xenopus
Mol.Wt.: 135kDa; 135kD(Calculated).
Uniprot: Q70E73
RRID: AB_2843000

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(86%), Rabbit(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RAPH1 Antibody detects endogenous levels of total RAPH1.
RRID:
AB_2843000
Cite Format: Affinity Biosciences Cat# DF9806, RRID:AB_2843000.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

9430025M21Rik; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; C730009O10Rik; Lamellipodin; PREL-2; Proline-rich EVH1 ligand 2; Protein RMO1; RAPH1; RAPH1_HUMAN; Ras-associated and pleckstrin homology domains-containing protein 1;

Immunogens

Immunogen:
Uniprot:

Q70E73(RAPH1_HUMAN) >>Visit HPA database.

Gene(ID):
RAPH1(65059)
Expression:
Q70E73 RAPH1_HUMAN:

Isoform RMO1-RAPH1 is ubiquitously expressed with highest levels in brain, heart, ovary and developing embryo. Isoform RMO1 is widely expressed with highest levels in liver. Low expression in B-cells.

Sequence:
MEQLSDEEIDHGAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDSDKPMEPVKRSPLRQETNMANFSYRFSIYNLNEALNQGETVDLDALMADLCSIEQELSSIGSGNSKRQITETKATQKLPVSRHTLKHGTLKGLSSSSNRIAKPSHASYSLDDVTAQLEQASLSMDEAAQQSVLEDTKPLVTNQHRRTASAGTVSDAEVHSISNSSHSSITSAASSMDSLDIDKVTRPQELDLTHQGQPITEEEQAAKLKAEKIRVALEKIKEAQVKKLVIRVHMSDDSSKTMMVDERQTVRQVLDNLMDKSHCGYSLDWSLVETVSELQMERIFEDHENLVENLLNWTRDSQNKLIFMERIEKYALFKNPQNYLLGKKETAEMADRNKEVLLEECFCGSSVTVPEIEGVLWLKDDGKKSWKKRYFLLRASGIYYVPKGKAKVSRDLVCFLQLDHVNVYYGQDYRNKYKAPTDYCLVLKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQLYMNYQEALKRTESAYDWTSLSSSSIKSGSSSSSIPESQSNHSNQSDSGVSDTQPAGHVRSQSIVSSVFSEAWKRGTQLEESSKARMESMNRPYTSLVPPLSPQPKIVTPYTASQPSPPLPPPPPPPPPPPPPPPPPPPPLPSQSAPSAGSAAPMFVKYSTITRLQNASQHSGALFKPPTPPVMQSQSVKPQILVPPNGVVPPPPPPPPPPTPGSAMAQLKPAPCAPSLPQFSAPPPPLKIHQVQHITQVAPPTPPPPPPIPAPLPPQAPPKPLVTIPAPTSTKTVAPVVTQAAPPTPTPPVPPAKKQPAFPASYIPPSPPTPPVPVPPPTLPKQQSFCAKPPPSPLSPVPSVVKQIASQFPPPPTPPAMESQPLKPVPANVAPQSPPAVKAKPKWQPSSIPVPSPDFPPPPPESSLVFPPPPPSPVPAPPPPPPPTASPTPDKSGSPGKKTSKTSSPGGKKPPPTPQRNSSIKSSSGAEHPEPKRPSVDSLVSKFTPPAESGSPSKETLPPPAAPPKPGKLNLSGVNLPGVLQQGCVSAKAPVLSGRGKDSVVEFPSPPSDSDFPPPPPETELPLPPIEIPAVFSGNTSPKVAVVNPQPQQWSKMSVKKAPPPTRPKRNDSTRLTQAEISEQPTMATVVPQVPTSPKSSLSVQPGFLADLNRTLQRKSITRHGSLSSRMSRAEPTATMDDMALPPPPPELLSDQQKAGYGGSHISGYATLRRGPPPAPPKRDQNTKLSRDW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
100
Rabbit
88
Bovine
86
Pig
0
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q70E73 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
S17 Phosphorylation
S41 Phosphorylation
S54 Phosphorylation
K116 Ubiquitination
T118 Phosphorylation
K120 Ubiquitination
T127 Phosphorylation
K129 Methylation
T132 Phosphorylation
K134 Methylation
K134 Ubiquitination
S140 Phosphorylation
S147 Phosphorylation
S150 Phosphorylation
S152 Phosphorylation
S192 Phosphorylation
S203 Phosphorylation
S205 Phosphorylation
S207 Phosphorylation
S208 Phosphorylation
S211 Phosphorylation
K250 Acetylation
K250 Ubiquitination
K270 Acetylation
S281 Phosphorylation
S282 Phosphorylation
Y366 Phosphorylation
S423 Phosphorylation
Y426 Phosphorylation P00519 (ABL1)
Y456 Phosphorylation P00519 (ABL1)
Y466 Phosphorylation
Y481 Phosphorylation
K483 Ubiquitination
Y510 Phosphorylation
Y513 Phosphorylation P00519 (ABL1)
Y524 Phosphorylation
S533 Phosphorylation
S542 Phosphorylation
S569 Phosphorylation
S571 Phosphorylation
S574 Phosphorylation
S575 Phosphorylation
S578 Phosphorylation
K582 Acetylation
K592 Ubiquitination
S597 Phosphorylation
Y602 Phosphorylation
T603 Phosphorylation
S604 Phosphorylation
S610 Phosphorylation P28482 (MAPK1)
Y667 Phosphorylation
T762 Phosphorylation
T784 O-Glycosylation
T805 Phosphorylation
S822 Phosphorylation
Y823 Phosphorylation
S827 Phosphorylation
T830 Phosphorylation
T839 Phosphorylation
S845 Phosphorylation
S853 Phosphorylation
S856 Phosphorylation
S860 Phosphorylation
S867 Phosphorylation
T874 Phosphorylation
S880 Phosphorylation
S894 Phosphorylation
S933 Phosphorylation
T945 Phosphorylation
T949 Phosphorylation
S965 Phosphorylation
T974 Phosphorylation
S980 Phosphorylation
S996 Phosphorylation
T1005 Phosphorylation
S1010 Phosphorylation
S1012 Phosphorylation
S1014 Phosphorylation
T1017 Phosphorylation
S1033 Phosphorylation
S1047 Phosphorylation
S1054 Phosphorylation
S1060 Phosphorylation
S1066 Phosphorylation
S1130 Phosphorylation
T1131 Phosphorylation
T1143 Phosphorylation
T1153 Phosphorylation
S1154 Phosphorylation
T1172 Phosphorylation
S1183 Phosphorylation
S1185 Phosphorylation
S1224 Phosphorylation
Y1226 Phosphorylation P00519 (ABL1)
T1228 Phosphorylation

Research Backgrounds

Function:

Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion.

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium. Cell projection>Filopodium. Cytoplasm>Cytoskeleton.
Note: Recruited to the membrane, via the PH domain, by the phosphoinositide, PI(3,4)P2. Colocalizes with ENAH/VASP at the tips of lamellipodia and filopodia. Also colocalizes with the pathogens, Vaccinia and Enteropathogenic E.coli (EPEC) at the interface between the pathogen and their actin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform RMO1-RAPH1 is ubiquitously expressed with highest levels in brain, heart, ovary and developing embryo. Isoform RMO1 is widely expressed with highest levels in liver. Low expression in B-cells.

Subunit Structure:

Interacts with EVL and VASP and targets them to the leading edge. Interacts (via Ras associating and PH domains) with RAC1.

Family&Domains:

Belongs to the MRL family.

Restrictive clause

 

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