Product: SFRS14 Antibody
Catalog: DF9792
Description: Rabbit polyclonal antibody to SFRS14
Application: WB IF/ICC
Reactivity: Human
Prediction: Bovine, Sheep
Mol.Wt.: 116kDa; 120kD(Calculated).
Uniprot: Q8IX01
RRID: AB_2842986

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(83%), Sheep(83%)
Clonality:
Polyclonal
Specificity:
SFRS14 Antibody detects endogenous levels of total SFRS14.
RRID:
AB_2842986
Cite Format: Affinity Biosciences Cat# DF9792, RRID:AB_2842986.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Arginine/serine rich splicing factor 14; arginine/serine-rich 14; Arginine/serine-rich-splicing factor 14; DKFZp779L2418; KIAA0365; Splicing factor; Splicing factor, arginine/serine-rich 14; Sugp2; SUGP2_HUMAN; SURP and G-patch domain-containing protein 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8IX01 SUGP2_HUMAN:

Detected in adult testis, and in fetal brain and kidney.

Sequence:
MAARRITQETFDAVLQEKAKRYHMDASGEAVSETLQFKAQDLLRAVPRSRAEMYDDVHSDGRYSLSGSVAHSRDAGREGLRSDVFPGPSFRSSNPSISDDSYFRKECGRDLEFSHSDSRDQVIGHRKLGHFRSQDWKFALRGSWEQDFGHPVSQESSWSQEYSFGPSAVLGDFGSSRLIEKECLEKESRDYDVDHPGEADSVLRGGSQVQARGRALNIVDQEGSLLGKGETQGLLTAKGGVGKLVTLRNVSTKKIPTVNRITPKTQGTNQIQKNTPSPDVTLGTNPGTEDIQFPIQKIPLGLDLKNLRLPRRKMSFDIIDKSDVFSRFGIEIIKWAGFHTIKDDIKFSQLFQTLFELETETCAKMLASFKCSLKPEHRDFCFFTIKFLKHSALKTPRVDNEFLNMLLDKGAVKTKNCFFEIIKPFDKYIMRLQDRLLKSVTPLLMACNAYELSVKMKTLSNPLDLALALETTNSLCRKSLALLGQTFSLASSFRQEKILEAVGLQDIAPSPAAFPNFEDSTLFGREYIDHLKAWLVSSGCPLQVKKAEPEPMREEEKMIPPTKPEIQAKAPSSLSDAVPQRADHRVVGTIDQLVKRVIEGSLSPKERTLLKEDPAYWFLSDENSLEYKYYKLKLAEMQRMSENLRGADQKPTSADCAVRAMLYSRAVRNLKKKLLPWQRRGLLRAQGLRGWKARRATTGTQTLLSSGTRLKHHGRQAPGLSQAKPSLPDRNDAAKDCPPDPVGPSPQDPSLEASGPSPKPAGVDISEAPQTSSPCPSADIDMKTMETAEKLARFVAQVGPEIEQFSIENSTDNPDLWFLHDQNSSAFKFYRKKVFELCPSICFTSSPHNLHTGGGDTTGSQESPVDLMEGEAEFEDEPPPREAELESPEVMPEEEDEDDEDGGEEAPAPGGAGKSEGSTPADGLPGEAAEDDLAGAPALSQASSGTCFPRKRISSKSLKVGMIPAPKRVCLIQEPKVHEPVRIAYDRPRGRPMSKKKKPKDLDFAQQKLTDKNLGFQMLQKMGWKEGHGLGSLGKGIREPVSVGTPSEGEGLGADGQEHKEDTFDVFRQRMMQMYRHKRANK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
83
Sheep
83
Pig
75
Horse
75
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IX01 As Substrate

Site PTM Type Enzyme
T7 Phosphorylation Q13535 (ATR)
T10 Phosphorylation
K18 Ubiquitination
K38 Ubiquitination
S49 Phosphorylation
Y54 Phosphorylation
S59 Phosphorylation
S93 Phosphorylation
S96 Phosphorylation
S101 Phosphorylation
S114 Phosphorylation
K127 Ubiquitination
S133 Phosphorylation
K137 Ubiquitination
S143 Phosphorylation
Y162 Phosphorylation
K181 Ubiquitination
C183 S-Nitrosylation
K186 Ubiquitination
S201 Phosphorylation
R204 Methylation
R212 Methylation
R214 Methylation
S224 Phosphorylation
K228 Ubiquitination
K238 Ubiquitination
K243 Ubiquitination
T262 Phosphorylation
K264 Sumoylation
K264 Ubiquitination
K273 Ubiquitination
T275 Phosphorylation
S277 Phosphorylation
T281 Phosphorylation
T284 Phosphorylation
K297 Ubiquitination
K305 Ubiquitination
K313 Ubiquitination
S315 Phosphorylation
K321 Ubiquitination
K342 Ubiquitination
S348 Phosphorylation
K370 Ubiquitination
K389 Ubiquitination
K394 Ubiquitination
K409 Ubiquitination
K415 Ubiquitination
K423 Ubiquitination
K427 Ubiquitination
Y428 Phosphorylation
T458 Phosphorylation
K478 Ubiquitination
S510 Phosphorylation
K532 Ubiquitination
K545 Ubiquitination
K546 Acetylation
K546 Ubiquitination
K557 Acetylation
T562 Phosphorylation
K563 Acetylation
S572 Phosphorylation
S573 Phosphorylation
S575 Phosphorylation
K595 Ubiquitination
S603 Phosphorylation
K605 Acetylation
K605 Ubiquitination
K611 Ubiquitination
K633 Ubiquitination
K650 Ubiquitination
K673 Ubiquitination
R689 Methylation
S706 Phosphorylation
T708 Phosphorylation
S726 Phosphorylation
S745 Phosphorylation
S750 Phosphorylation
S754 Phosphorylation
S757 Phosphorylation
S772 Phosphorylation
S773 Phosphorylation
K790 Ubiquitination
S806 Phosphorylation
S810 Phosphorylation
T811 Phosphorylation
T852 Phosphorylation
S860 Phosphorylation
S887 Phosphorylation
S940 Phosphorylation
Y985 Phosphorylation
K1008 Ubiquitination
K1012 Ubiquitination
K1021 Methylation
K1025 Ubiquitination
S1032 Phosphorylation
K1035 Ubiquitination
T1045 Phosphorylation
S1047 Phosphorylation

Research Backgrounds

Function:

May play a role in mRNA splicing.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in adult testis, and in fetal brain and kidney.

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