APOBEC3A/B Antibody - #DF9776
| Product: | APOBEC3A/B Antibody |
| Catalog: | DF9776 |
| Description: | Rabbit polyclonal antibody to APOBEC3A/B |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Rabbit |
| Mol.Wt.: | 23kD(A), 46kD(B); 23kD,46kD(Calculated). |
| Uniprot: | P31941 | Q9UH17 |
| RRID: | AB_2842971 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9776, RRID:AB_2842971.
Fold/Unfold
A3A; ABC3A_HUMAN; APOBEC3A; Apolipoprotein B mRNA editing enzyme; Apolipoprotein B mRNA editing enzyme, catalytic polypeptide like 3A; ARP3; bK150C2.1; Catalytic polypeptide like 3A; Phorbolin 1; Phorbolin-1; PHRBN; Probable DNA dC >dU editing enzyme APOBEC-3A; Probable DNA dC->dU-editing enzyme APOBEC-3A; A3B; APOBEC1L; Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B; ARCD3; ARP4; bK150C2.2; Cytidine deaminase; DJ742C19.2; DNA dC->dU-editing enzyme APOBEC-3B; FLJ21201; Phorbolin 1 related protein; Phorbolin 2; Phorbolin 2/3; Phorbolin 3; PHRBNL; Probable DNA dC>dU editing enzyme APOBEC3B;
Immunogens
Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia.
Q9UH17 ABC3B_HUMAN:Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. Also expressed at low levels in several other tissues.
- P31941 ABC3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSVKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDTFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN
- Q9UH17 ABC3B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLLWDTGVFRGQVYFKPQYHAEMCFLSWFCGNQLPAYKCFQITWFVSWTPCPDCVAKLAEFLSEHPNVTLTISAARLYYYWERDYRRALCRLSQAGARVTIMDYEEFAYCWENFVYNEGQQFMPWYKFDENYAFLHRTLKEILRYLMDPDTFTFNFNNDPLVLRRRQTYLCYEVERLDNGTWVLMDQHMGFLCNEAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFEYCWDTFVYRQGCPFQPWDGLEEHSQALSGRLRAILQNQGN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P31941/Q9UH17 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y130 | Phosphorylation | Uniprot | |
| Y132 | Phosphorylation | Uniprot | |
| Y136 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S46 | Phosphorylation | Uniprot | |
| R137 | Methylation | Uniprot | |
| Y178 | Phosphorylation | Uniprot | |
| Y191 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| Y313 | Phosphorylation | Uniprot | |
| Y315 | Phosphorylation | Uniprot | |
| Y319 | Phosphorylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| S334 | Phosphorylation | Uniprot |
Research Backgrounds
DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
Nucleus. Cytoplasm.
Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia.
Interacts with AGO2. Interacts with TRIB3 (via N-terminus).
Belongs to the cytidine and deoxycytidylate deaminase family.
DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons.
Nucleus.
Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. Also expressed at low levels in several other tissues.
Homodimer. Interacts with APOBEC3G. Does not interact with APOBEC1.
The CMP/dCMP deaminase domain 1 mediates RNA binding, RNA-dependent oligomerization and virion incorporation whereas the CMP/dCMP deaminase domain 2 confers deoxycytidine deaminase activity and substrate sequence specificity.
Belongs to the cytidine and deoxycytidylate deaminase family.
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