PRDM10 Antibody | Affinity Biosciences

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Western blot analysis of PRDM10 using K562 whole cell lysates.

DF9772 at 1/100 staining Rat brain tissue by IHC-P.

Product:	PRDM10 Antibody
Catalog:	DF9772
Description:	Rabbit polyclonal antibody to PRDM10
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Dog
Mol.Wt.:	130 kDa; 130kD(Calculated).
Uniprot:	Q9NQV6
RRID:	AB_2842967

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Dog(86%)

Clonality:

Polyclonal

Specificity:

PRDM10 Antibody detects endogenous levels of total PRDM10.

RRID:

AB_2842967
Cite Format: Affinity Biosciences Cat# DF9772, RRID:AB_2842967.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

KIAA1231; MGC131802; PFM7; PR domain containing 10; PR domain containing protein 10; PR domain family member 7; PR domain zinc finger protein 10; PR domain-containing protein 10; PRD10_HUMAN; PRDM zinc finger transcription factor; prdm10; TRIS; Tristanin;

Immunogens

Immunogen:

Uniprot:

Q9NQV6(PRD10_HUMAN) >>Visit HPA database.

Gene(ID):

PRDM10(56980)

Sequence:

Q9NQV6 PRD10_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDSKDESSHVWPTSAEHEQNAAQVHFVPDTGTVAQIVYTDDQVRPPQQVVYTADGASYTSVDGPEHTLVYIHPVEAAQTLFTDPGQVAYVQQDATAQQASLPVHNQVLPSIESVDGSDPLATLQTPLGRLEAKEEEDEDEDEDTEEDEEEDGEDTDLDDWEPDPPRPFDPHDLWCEECNNAHASVCPKHGPLHPIPNRPVLTRARASLPLVLYIDRFLGGVFSKRRIPKRTQFGPVEGPLVRGSELKDCYIHLKVSLDKGDRKERDLHEDLWFELSDETLCNWMMFVRPAQNHLEQNLVAYQYGHHVYYTTIKNVEPKQELKVWYAASYAEFVNQKIHDISEEERKVLREQEKNWPCYECNRRFISSEQLQQHLNSHDEKLDVFSRTRGRGRGRGKRRFGPGRRPGRPPKFIRLEITSENGEKSDDGTQDLLHFPTKEQFDEAEPATLNGLDQPEQTTIPIPQLPQETQSSLEHEPETHTLHLQPQHEESVVPTQSTLTADDMRRAKRIRLELQNAALQHLFIRKSFRPFKCLQCGKAFREKDKLDQHLRFHGREGNCPLTCDLCNKGFISSTSLESHMKLHSDQKTYSCIFCPESFDRLDLLKDHVAIHINDGYFTCPTCKKRFPDFIQVKKHVRSFHSEKIYQCTECDKAFCRPDKLRLHMLRHSDRKDFLCSTCGKQFKRKDKLREHMQRMHNPEREAKKADRISRSKTFKPRITSTDYDSFTFKCRLCMMGFRRRGMLVNHLSKRHPDMKIEEVPELTLPIIKPNRDYFCQYCDKVYKSASKRKAHILKNHPGAELPPSIRKLRPAGPGEPDPMLSTHTQLTGTIATPPVCCPHCSKQYSSKTKMVQHIRKKHPEFAQLSNTIHTPLTTAVISATPAVLTTDSATGETVVTTDLLTQAMTELSQTLTTDYRTPQGDYQRIQYIPVSQSASGLQQPQHIQLQVVQVASATSPHQSQQSTVDVGQLHDPQPYPQHAIQVQHIQVSGQPLSPSAQQAQQGLSPSHIQGSSSTQGQALQQQQQQQQNSSVQHTYLPSAWNSFRGYSSEIQMMTLPPGQFVITDSGVATPVTTGQVKAVTSGHYVLSESQSELEEKQTSALSGGVQVEPPAHSDSLDPQTNSQQQTTQYIITTTTNGNGSSEVHITKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NQV6 As Substrate

Q9NQV6

Site	PTM Type	Source
S341	Phosphorylation	Uniprot
S418	Phosphorylation	Uniprot
S424	Phosphorylation	Uniprot
T428	Phosphorylation	Uniprot
S637	Phosphorylation	Uniprot
S640	Phosphorylation	Uniprot
K658	Acetylation	Uniprot
S667	Phosphorylation	Uniprot
S708	Phosphorylation	Uniprot
S724	Phosphorylation	Uniprot
K767	Ubiquitination	Uniprot
S803	Phosphorylation	Uniprot
S1088	Phosphorylation	Uniprot

Research Backgrounds

Q9NQV6_PRD10_HUMAN

Function:

May be involved in transcriptional regulation.

Subcellular Location:

Nucleus.

Family&Domains:

The SET domain is degenerated, suggesting that it has lost methyltransferase activity.

Belongs to the class V-like SAM-binding methyltransferase superfamily.

References

1). Nasopharyngeal Carcinoma Protein Interaction Mapping Analysis via Proteomic Approaches. Asian Pacific Journal of Cancer Prevention, 2018 (PubMed: 29582644)

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PRDM10 Antibody - #DF9772