PIGA Antibody | Affinity Biosciences

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Western blot analysis of extracts from VERO cells, using PIGA Antibody.

Product:	PIGA Antibody
Catalog:	DF9742
Description:	Rabbit polyclonal antibody to PIGA
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Mol.Wt.:	54 kDa; 54kD(Calculated).
Uniprot:	P37287
RRID:	AB_2842937

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Clonality:

Polyclonal

Specificity:

PIGA Antibody detects endogenous levels of total PIGA.

RRID:

AB_2842937
Cite Format: Affinity Biosciences Cat# DF9742, RRID:AB_2842937.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Class A GlcNAc inositol phospholipid assembly protein; GlcNAc PI synthesis protein; GlcNAc-PI synthesis protein; GPI3; MCAHS2; Phosphatidylinositol glycan anchor biosynthesis class A; Phosphatidylinositol glycan biosynthesis class A protein; Phosphatidylinositol N-acetylglucosaminyltransferase subunit A; Phosphatidylinositol-glycan biosynthesis class A protein; PIG A; PIG-A; Piga; PIGA_HUMAN;

Immunogens

Immunogen:

Uniprot:

P37287(PIGA_HUMAN) >>Visit HPA database.

Gene(ID):

PIGA(5277)

Sequence:

P37287 PIGA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MACRGGAGNGHRASATLSRVSPGSLYTCRTRTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVIIVTHAYGNRKGIRYLTSGLKVYYLPLKVMYNQSTATTLFHSLPLLRYIFVRERVTIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRHDSITIVVVSRLVYRKGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRERYQLHDRVRLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTRVGGIPEVLPENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAERTEKVYDRVSVEAVLPMDKRLDRLISHCGPVTGYIFALLAVFNFLFLIFLRWMTPDSIIDVAIDATGPRGAWTNNYSHSKRGGENNEISETR

PTMs - P37287 As Substrate

P37287

Site	PTM Type	Source
R4	Methylation	Uniprot
S21	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
T27	Phosphorylation	Uniprot
T32	Phosphorylation	Uniprot
Y254	Phosphorylation	Uniprot
K293	Ubiquitination	Uniprot
S354	Phosphorylation	Uniprot
K361	Ubiquitination	Uniprot
K367	Ubiquitination	Uniprot
Y385	Phosphorylation	Uniprot
K396	Acetylation	Uniprot

Research Backgrounds

P37287_PIGA_HUMAN

Function:

Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Subunit Structure:

Associates with PIGC, PIGH, PIGP, PIGQ and DPM2. The latter is not essential for activity. Interacts directly with PIGY.

Family&Domains:

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > Glycosylphosphatidylinositol (GPI)-anchor biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PIGA Antibody - #DF9742