PEX6 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of PEX6 using A549 whole cell lysates.

Product:	PEX6 Antibody
Catalog:	DF9738
Description:	Rabbit polyclonal antibody to PEX6
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	104 kDa; 104kD(Calculated).
Uniprot:	Q13608
RRID:	AB_2842933

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(100%), Horse(100%), Sheep(90%), Rabbit(100%), Dog(100%), Chicken(90%)

Clonality:

Polyclonal

Specificity:

PEX6 Antibody detects endogenous levels of total PEX6.

RRID:

AB_2842933
Cite Format: Affinity Biosciences Cat# DF9738, RRID:AB_2842933.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

PAF 2; PAF2; PBD4A; PDB4B; Peroxin 6; Peroxisomal AAA type ATPase 1; Peroxisomal biogenesis factor 6; Peroxisomal type ATPase 1; Peroxisome assembly factor 2; PXAAA1;

Immunogens

Immunogen:

Uniprot:

Q13608(PEX6_HUMAN) >>Visit HPA database.

Gene(ID):

PEX6(5190)

Expression:

Q13608 PEX6_HUMAN:

Expressed in the retina, at higher levels in the photoreceptor layer at the joint between the outer and inner segments.

Sequence:

Q13608 PEX6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALAVLRVLEPFPTETPPLAVLLPPGGPWPAAELGLVLALRPAGESPAGPALLVAALEGPDAGTEEQGPGPPQLLVSRALLRLLALGSGAWVRARAVRRPPALGWALLGTSLGPGLGPRVGPLLVRRGETLPVPGPRVLETRPALQGLLGPGTRLAVTELRGRARLCPESGDSSRPPPPPVVSSFAVSGTVRRLQGVLGGTGDSLGVSRSCLRGLGLFQGEWVWVAQARESSNTSQPHLARVQVLEPRWDLSDRLGPGSGPLGEPLADGLALVPATLAFNLGCDPLEMGELRIQRYLEGSIAPEDKGSCSLLPGPPFARELHIEIVSSPHYSTNGNYDGVLYRHFQIPRVVQEGDVLCVPTIGQVEILEGSPEKLPRWREMFFKVKKTVGEAPDGPASAYLADTTHTSLYMVGSTLSPVPWLPSEESTLWSSLSPPGLEALVSELCAVLKPRLQPGGALLTGTSSVLLRGPPGCGKTTVVAAACSHLGLHLLKVPCSSLCAESSGAVETKLQAIFSRARRCRPAVLLLTAVDLLGRDRDGLGEDARVMAVLRHLLLNEDPLNSCPPLMVVATTSRAQDLPADVQTAFPHELEVPALSEGQRLSILRALTAHLPLGQEVNLAQLARRCAGFVVGDLYALLTHSSRAACTRIKNSGLAGGLTEEDEGELCAAGFPLLAEDFGQALEQLQTAHSQAVGAPKIPSVSWHDVGGLQEVKKEILETIQLPLEHPELLSLGLRRSGLLLHGPPGTGKTLLAKAVATECSLTFLSVKGPELINMYVGQSEENVREVFARARAAAPCIIFFDELDSLAPSRGRSGDSGGVMDRVVSQLLAELDGLHSTQDVFVIGATNRPDLLDPALLRPGRFDKLVFVGANEDRASQLRVLSAITRKFKLEPSVSLVNVLDCCPPQLTGADLYSLCSDAMTAALKRRVHDLEEGLEPGSSALMLTMEDLLQAAARLQPSVSEQELLRYKRIQRKFAAC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Dog

100

Rabbit

100

Pig

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13608 As Substrate

Q13608

Site	PTM Type	Source
R93	Methylation	Uniprot
R95	Methylation	Uniprot
R119	Methylation	Uniprot
R161	Methylation	Uniprot
K306	Ubiquitination	Uniprot
S371	Phosphorylation	Uniprot
K374	Ubiquitination	Uniprot
K714	Ubiquitination	Uniprot
K715	Ubiquitination	Uniprot
K750	Ubiquitination	Uniprot

Research Backgrounds

Q13608_PEX6_HUMAN

Function:

Involved in peroxisome biosynthesis. Required for stability of the PTS1 receptor. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes.

Subcellular Location:

Cytoplasm. Peroxisome membrane. Cell projection>Cilium>Photoreceptor outer segment.
Note: Associated with peroxisomal membranes. Localized at the base of the outer segment of photoreceptor cells (PubMed:26593283).

Tissue Specificity:

Expressed in the retina, at higher levels in the photoreceptor layer at the joint between the outer and inner segments.

Subunit Structure:

Interacts directly with PEX26 and PEX1. Mediates the indirect interaction between PEX1 and PEX26. Interacts with ZFAND6.

Family&Domains:

Belongs to the AAA ATPase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

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For Research Use Only.
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PEX6 Antibody - #DF9738