CROT Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from MDA-MB-231 cells, using CROT Antibody.

Product:	CROT Antibody
Catalog:	DF9733
Description:	Rabbit polyclonal antibody to CROT
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	70 kDa; 70kD(Calculated).
Uniprot:	Q9UKG9
RRID:	AB_2842928

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(92%)

Clonality:

Polyclonal

Specificity:

CROT Antibody detects endogenous levels of total CROT.

RRID:

AB_2842928
Cite Format: Affinity Biosciences Cat# DF9733, RRID:AB_2842928.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Carnitine O octanoyltransferase; COT; CROT; OCTC_HUMAN; Peroxisomal carnitine acyltransferase; Peroxisomal carnitine O octanoyltransferase; Peroxisomal carnitine O-octanoyltransferase;

Immunogens

Immunogen:

Uniprot:

Q9UKG9(OCTC_HUMAN) >>Visit HPA database.

Gene(ID):

CROT(54677)

Sequence:

Q9UKG9 OCTC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENQLAKSTEERTFQYQDSLPSLPVPSLEESLKKYLESVKPFANQEEYKKTEEIVQKFQSGIGEKLHQKLLERAKGKRNWLEEWWLNVAYLDVRIPSQLNVNFAGPAAHFEHYWPPKEGTQLERGSITLWHNLNYWQLLRKEKVPVHKVGNTPLDMNQFRMLFSTCKVPGITRDSIMNYFRTESEGRSPNHIVVLCRGRAFVFDVIHEGCLVTPPELLRQLTYIHKKCHSEPDGPGIAALTSEERTRWAKAREYLIGLDPENLALLEKIQSSLLVYSMEDSSPHVTPEDYSEIIAAILIGDPTVRWGDKSYNLISFSNGVFGCNCDHAPFDAMIMVNISYYVDEKIFQNEGRWKGSEKVRDIPLPEELIFIVDEKVLNDINQAKAQYLREASDLQIAAYAFTSFGKKLTKNKMLHPDTFIQLALQLAYYRLHGHPGCCYETAMTRHFYHGRTETMRSCTVEAVRWCQSMQDPSVNLRERQQKMLQAFAKHNKMMKDCSAGKGFDRHLLGLLLIAKEEGLPVPELFTDPLFSKSGGGGNFVLSTSLVGYLRVQGVVVPMVHNGYGFFYHIRDDRFVVACSAWKSCPETDAEKLVQLTFCAFHDMIQLMNSTHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Rabbit

100

Bovine

Sheep

Dog

Zebrafish

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UKG9 As Substrate

Q9UKG9

Site	PTM Type	Source
M1	Acetylation	Uniprot
Y35	Phosphorylation	Uniprot
S38	Phosphorylation	Uniprot
K40	Ubiquitination	Uniprot
K57	Ubiquitination	Uniprot
K65	Ubiquitination	Uniprot
K69	Ubiquitination	Uniprot
K167	Ubiquitination	Uniprot
K358	Acetylation	Uniprot
T409	Phosphorylation	Uniprot
Y428	Phosphorylation	Uniprot
Y429	Phosphorylation	Uniprot
S533	Phosphorylation	Uniprot

Research Backgrounds

Q9UKG9_OCTC_HUMAN

Function:

Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester.

Subcellular Location:

Peroxisome.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the carnitine/choline acetyltransferase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CROT Antibody - #DF9733