Product: NOL9 Antibody
Catalog: DF9721
Description: Rabbit polyclonal antibody to NOL9
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 79 kDa; 79kD(Calculated).
Uniprot: Q5SY16
RRID: AB_2842916

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NOL9 Antibody detects endogenous levels of total NOL9.
RRID:
AB_2842916
Cite Format: Affinity Biosciences Cat# DF9721, RRID:AB_2842916.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ23323; MGC131821; MGC138483; NET6; NOL9; Nucleolar protein 9;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MADSGLLLKRGSCRSTWLRVRKARPQLILSRRPRRRLGSLRWCGRRRLRWRLLQAQASGVDWREGARQVSRAAAARRPNTATPSPIPSPTPASEPESEPELESASSCHRPLLIPPVRPVGPGRALLLLPVEQGFTFSGICRVTCLYGQVQVFGFTISQGQPAQDIFSVYTHSCLSIHALHYSQPEKSKKELKREARNLLKSHLNLDDRRWSMQNFSPQCSIVLLEHLKTATVNFITSYPGSSYIFVQESPTPQIKPEYLALRSVGIRREKKRKGLQLTESTLSALEELVNVSCEEVDGCPVILVCGSQDVGKSTFNRYLINHLLNSLPCVDYLECDLGQTEFTPPGCISLLNITEPVLGPPFTHLRTPQKMVYYGKPSCKNNYENYIDIVKYVFSAYKRESPLIVNTMGWVSDQGLLLLIDLIRLLSPSHVVQFRSDHSKYMPDLTPQYVDDMDGLYTKSKTKMRNRRFRLAAFADALEFADEEKESPVEFTGHKLIGVYTDFAFRITPRNRESHNKILRDLSILSYLSQLQPPMPKPLSPLHSLTPYQVPFNAVALRITHSDVAPTHILYAVNASWVGLCKIQDDVRGYTNGPILLAQTPICDCLGFGICRGIDMEKRLYHILTPVPPEELRTVNCLLVGAIAIPHCVLKCQRGIEGTVPYVTTDYNFKLPGASEKIGAREPEEAHKEKPYRRPKFCRKMK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5SY16 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation
K9 Methylation
K9 Ubiquitination
K22 Ubiquitination
S84 Phosphorylation
S88 Phosphorylation
S93 Phosphorylation
S97 Phosphorylation
K200 Methylation
K200 Ubiquitination
S249 Phosphorylation
K376 Ubiquitination
K380 Ubiquitination
Y386 Phosphorylation
Y392 Phosphorylation
S395 Phosphorylation
Y397 Phosphorylation
S427 Phosphorylation
K440 Ubiquitination
K459 Ubiquitination
K485 Sumoylation
K485 Ubiquitination
S487 Phosphorylation
T492 Phosphorylation
Y571 Phosphorylation
K618 Ubiquitination
K651 Ubiquitination
K670 Ubiquitination
K677 Ubiquitination

Research Backgrounds

Function:

Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA.

Subcellular Location:

Nucleus. Nucleus>Nucleolus.
Note: Colocalizes with pre-60S rRNP particles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PELP1, WDR18 and SENP3.

Family&Domains:

Belongs to the Clp1 family. NOL9/GRC3 subfamily.

Restrictive clause

 

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For Research Use Only.
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