Product: TNP2 Antibody
Catalog: DF9713
Description: Rabbit polyclonal antibody to TNP2
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Dog
Mol.Wt.: 16 kDa; 16kD(Calculated).
Uniprot: Q05952
RRID: AB_2842908

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TNP2 Antibody detects endogenous levels of total TNP2.
RRID:
AB_2842908
Cite Format: Affinity Biosciences Cat# DF9713, RRID:AB_2842908.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Nuclear transition protein 2; TNP2; TP 2; TP2; Transition protein 2 (during histone to protamine replacement);

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q05952 STP2_HUMAN:

Expressed by spermatids (at protein level).

Sequence:
MDTQTHSLPITHTQLHSNSQPQSRTCTRHCQTFSQSCRQSHRGSRSQSSSQSPASHRNPTGAHSSSGHQSQSPNTSPPPKRHKKTMNSHHSPMRPTILHCRCPKNRKNLEGKLKKKKMAKRIQQVYKTKTRSSGWKSN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05952 As Substrate

Site PTM Type Enzyme
S17 Phosphorylation

Research Backgrounds

Function:

Plays a key role in the replacement of histones to protamine in the elongating spermatids of mammals. In condensing spermatids, loaded onto the nucleosomes, where it promotes the recruitment and processing of protamines, which are responsible for histone eviction.

Subcellular Location:

Nucleus. Chromosome.
Note: Loaded onto the nucleosomes of condensing spermatids (By similarity). Nuclear import is ATP-dependent and nucleolar localization requires the protein to be phosphorylated (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed by spermatids (at protein level).

Family&Domains:

Belongs to the nuclear transition protein 2 family.

Restrictive clause

 

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For Research Use Only.
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