DFNA5/GSDME Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of extracts from Hela, using DFNA5 Antibody.

DF9705 at 1/100 staining Rat liver tissue by IHC-P.

DF9705 staining HepG2 by IF/ICC.

DF9705 staining HepG2 cells by IF/ICC.

Product:	DFNA5/GSDME Antibody
Catalog:	DF9705
Description:	Rabbit polyclonal antibody to DFNA5/GSDME
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	55 kDa; 55kD(Calculated).
Uniprot:	O60443
RRID:	AB_2842900

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

DFNA5/GSDME Antibody detects endogenous levels of total DFNA5/GSDME.

RRID:

AB_2842900
Cite Format: Affinity Biosciences Cat# DF9705, RRID:AB_2842900.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2310037D07Rik; 4932441K13Rik; Deafness, autosomal dominant 5; Deafness, autosomal dominant 5 protein; DFNA5; DFNA5 gene; DFNA5_HUMAN; Dfna5h; EG14210; Fin15; ICERE 1; ICERE-1; Inversely correlated with estrogen receptor expression 1; Non-syndromic hearing impairment protein 5; Nonsyndromic hearing impairment protein; Gasdermin-E;GSDME_HUMAN;ICERE1;

Immunogens

Immunogen:

Uniprot:

O60443(GSDME_HUMAN) >>Visit HPA database.

Gene(ID):

DFNA5(1687)

Expression:

O60443 GSDME_HUMAN:

Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.

Sequence:

O60443 GSDME_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

PTMs - O60443 As Substrate

O60443

Site	PTM Type	Source
K4	Ubiquitination	Uniprot
T6	Phosphorylation	Uniprot
Y80	Phosphorylation	Uniprot
K83	Ubiquitination	Uniprot
K98	Ubiquitination	Uniprot
K100	Ubiquitination	Uniprot
S106	Phosphorylation	Uniprot
T117	Phosphorylation	Uniprot
S132	Phosphorylation	Uniprot
C156	S-Nitrosylation	Uniprot
K248	Ubiquitination	Uniprot
S252	Phosphorylation	Uniprot
Y254	Phosphorylation	Uniprot
K286	Ubiquitination	Uniprot
K440	Ubiquitination	Uniprot
K467	Ubiquitination	Uniprot

Research Backgrounds

O60443_GSDME_HUMAN

Function:

Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53.

Switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis. Produced by the cleavage of GSDME by CASP3, perforates cell membrane and thereby induces pyroptosis. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents. Exhibits bactericidal activity.

PTMs:

Cleavage at Asp-270 by CASP3 (mature and uncleaved precursor forms) relieves autoinhibition and is sufficient to initiate pyroptosis.

Subcellular Location:

Cell membrane.

Cytoplasm>Cytosol.

Tissue Specificity:

Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.

Subunit Structure:

The N-terminal moiety forms homooligomer; disulfide-linked. May form an 16-mer complex. Oligomerization occurs in the presence of membranes.

Family&Domains:

Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain, that is released upon cleavage by CASP3.

Belongs to the gasdermin family.

References

1). Neuronal-specific TNFAIP1 ablation attenuates postoperative cognitive dysfunction via targeting SNAP25 for K48-linked ubiquitination. Cell communication and signaling : CCS, 2023 (PubMed: 38102610) [IF=8.4]

2). Membrane Attack Complex C5b-9 Promotes Renal Tubular Epithelial Cell Pyroptosis in Trichloroethylene-Sensitized Mice. Frontiers in Pharmacology, 2022 (PubMed: 35656289) [IF=5.6]

3). Caspase-3 and gasdermin E mediate macrophage pyroptosis in periodontitis. Journal of periodontal research, 2024 (PubMed: 37885312) [IF=3.5]

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DFNA5/GSDME Antibody - #DF9705