NID2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from COS-7, using NID2 Antibody.

Product:	NID2 Antibody
Catalog:	DF9704
Description:	Rabbit polyclonal antibody to NID2
Application:	WB
Reactivity:	Human, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	151kDa; 151kD(Calculated).
Uniprot:	Q14112
RRID:	AB_2842899

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(90%)

Clonality:

Polyclonal

Specificity:

NID2 Antibody detects endogenous levels of total NID2.

RRID:

AB_2842899
Cite Format: Affinity Biosciences Cat# DF9704, RRID:AB_2842899.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

NID 2; NID-2; Nid2; NID2_HUMAN; Nidogen-2; Nidogen2; Osteonidogen;

Immunogens

Immunogen:

Uniprot:

Q14112(NID2_HUMAN) >>Visit HPA database.

Gene(ID):

NID2(22795)

Expression:

Q14112 NID2_HUMAN:

Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle.

Sequence:

Q14112 NID2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVKLANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRGRVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELNTFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGPYFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSFSHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEESSTLDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSIQPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTYNAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHLHVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPGSENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFTAHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPTTQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGTGVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRHTCILITPPANPCEDGSHTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENRCHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHIPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGTTPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLHGSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTMYWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Rabbit

Xenopus

Chicken

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14112 As Substrate

Q14112

Site	PTM Type	Source
T115	Phosphorylation	Uniprot
S116	Phosphorylation	Uniprot
S214	Phosphorylation	Uniprot
S237	Phosphorylation	Uniprot
S358	O-Glycosylation	Uniprot
S359	O-Glycosylation	Uniprot
S359	Phosphorylation	Uniprot
T360	O-Glycosylation	Uniprot
T365	O-Glycosylation	Uniprot
T369	O-Glycosylation	Uniprot
T369	Phosphorylation	Uniprot
S370	O-Glycosylation	Uniprot
T391	O-Glycosylation	Uniprot
S393	O-Glycosylation	Uniprot
S448	O-Glycosylation	Uniprot
Y449	Phosphorylation	Uniprot
S452	O-Glycosylation	Uniprot
T455	O-Glycosylation	Uniprot
T456	O-Glycosylation	Uniprot
S459	O-Glycosylation	Uniprot
S459	Phosphorylation	Uniprot
T479	O-Glycosylation	Uniprot
T502	Phosphorylation	Uniprot
Y504	Phosphorylation	Uniprot
T506	Phosphorylation	Uniprot
T773	Phosphorylation	Uniprot
S816	Phosphorylation	Uniprot
N1124	N-Glycosylation	Uniprot
S1218	Phosphorylation	Uniprot
T1331	Phosphorylation	Uniprot

Research Backgrounds

Q14112_NID2_HUMAN

Function:

Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.

PTMs:

Highly N- and O-glycosylated.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix>Basement membrane.

Tissue Specificity:

Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle.

Subunit Structure:

Interacts with LAMA2 (By similarity). Interacts with COL13A1.

References

1). Upregulated TIMP1 facilitates and coordinates myometrial contraction by decreasing collagens and cell adhesive capacity during human labor. Molecular Human Reproduction, 2023 (PubMed: 37774003) [IF=4.0]

Application: WB Species: Human Sample: MSMCs

Figure 6. Different cell adhesive properties in different human myometrial smooth muscle cells (hMSMCs). (A) Cell adhesive properties in nonpregnant, nonlabor and in-labor term hMSMCs. *IL versus NL, P

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For Research Use Only.
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NID2 Antibody - #DF9704