NPS Antibody | Affinity Biosciences

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Western blot analysis NPS using HepG2 whole cell lysates.

Product:	NPS Antibody
Catalog:	DF9702
Description:	Rabbit polyclonal antibody to NPS
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	10 kDa; 10kD(Calculated).
Uniprot:	P0C0P6
RRID:	AB_2842897

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(94%), Bovine(94%), Sheep(94%), Rabbit(94%), Dog(94%)

Clonality:

Polyclonal

Specificity:

NPS Antibody detects endogenous levels of total NPS.

RRID:

AB_2842897
Cite Format: Affinity Biosciences Cat# DF9702, RRID:AB_2842897.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Neuropeptide S; Nps; NPS_HUMAN;

Immunogens

Immunogen:

Uniprot:

P0C0P6(NPS_HUMAN) >>Visit HPA database.

Gene(ID):

NPS(594857)

Sequence:

P0C0P6 NPS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MISSVKLNLILVLSLSTMHVFWCYPVPSSKVSGKSDYFLILLNSCPTRLDRSKELAFLKPILEKMFVKRSFRNGVGTGMKKTSFQRAKS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Dog

Rabbit

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P0C0P6_NPS_HUMAN

Function:

Modulates arousal and anxiety. May play an important anorexigenic role (By similarity). Binds to its receptor NPSR1 with nanomolar affinity to increase intracellular calcium concentrations.

Subcellular Location:

Secreted.

References

1). Neuropeptide S and its receptor NPSR enhance the susceptibility of hosts to pseudorabies virus infection. Research in Veterinary Science, 2022 (PubMed: 35298925) [IF=2.4]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

NPS Antibody - #DF9702